Bullseye Analysis: A Fluorescence Microscopy Technique to Detect Local Changes in Intracellular Reactive Oxygen Species (ROS) Production (2022)
Journal Article
Hughes, J. G., Chisholm, D. R., Whiting, A., Girkin, J. M., & Ambler, C. A. (2023). Bullseye Analysis: A Fluorescence Microscopy Technique to Detect Local Changes in Intracellular Reactive Oxygen Species (ROS) Production. Microscopy and Microanalysis, 29(2), 529-539. https://doi.org/10.1093/micmic/ozac040

Reactive oxygen species (ROS) are naturally produced compounds that play important roles in cell signaling, gene regulation, and biological defense, including involvement in the oxidative burst that is central to the anti-microbial actions of macroph... Read More about Bullseye Analysis: A Fluorescence Microscopy Technique to Detect Local Changes in Intracellular Reactive Oxygen Species (ROS) Production.

Three-dimensional data capture and analysis of intact eye lenses evidences emmetropia-associated changes and strain-dependent differences in epithelial cell organization (2020)
Journal Article
Kalligeraki, A. A., Isted, A., Pal, R., Saunter, C., Girkin, J., Jarrin, M., …Quinlan, R. A. (2020). Three-dimensional data capture and analysis of intact eye lenses evidences emmetropia-associated changes and strain-dependent differences in epithelial cell organization. Scientific Reports, 10, Article 16898. https://doi.org/10.1038/s41598-020-73625-9

Organ and tissue development are highly coordinated processes; lens growth and functional integration into the eye (emmetropia) is a robust example. An epithelial monolayer covers the anterior hemisphere of the lens, and its organization is the key t... Read More about Three-dimensional data capture and analysis of intact eye lenses evidences emmetropia-associated changes and strain-dependent differences in epithelial cell organization.

Cellular localisation of structurally diverse diphenylacetylene fluorophores (2020)
Journal Article
Chisholm, D. R., Hughes, J. G., Blacker, T. S., Humann, R., Adams, C., Callaghan, D., …Whiting, A. (2020). Cellular localisation of structurally diverse diphenylacetylene fluorophores. Organic and Biomolecular Chemistry, 18(45), 9231-9245. https://doi.org/10.1039/d0ob01153c

Fluorescent probes are increasingly used as reporter molecules in a wide variety of biophysical experiments, but when designing new compounds it can often be difficult to anticipate the effect that changing chemical structure can have on cellular loc... Read More about Cellular localisation of structurally diverse diphenylacetylene fluorophores.

Photoactivated cell-killing involving a low molecular weight, donor-acceptor diphenylacetylene (2019)
Journal Article
Chisholm, D., Lamb, R., Pallett, T., Affleck, V., Holden, C., Marrison, J., …Ambler, C. A. (2019). Photoactivated cell-killing involving a low molecular weight, donor-acceptor diphenylacetylene. Chemical Science, 10(17), 4673-4683. https://doi.org/10.1039/c9sc00199a

Photoactivation of photosensitisers can be utilised to elicit the production of ROS, for potential therapeutic applications, including the destruction of diseased tissues and tumours. A novel class of photosensitiser, exemplified by DC324, has been d... Read More about Photoactivated cell-killing involving a low molecular weight, donor-acceptor diphenylacetylene.

An optical platform for the production, trapping, manipulation and visualization of ultra-low interfacial tension emulsion droplets (2013)
Conference Proceeding
Hargreaves, A. L., Kirby, A. K., Bain, C. D., Love, G. D., Bolognesi, G., Ces, O., …Ward, A. D. (2013). An optical platform for the production, trapping, manipulation and visualization of ultra-low interfacial tension emulsion droplets. In K. Dholakia, & G. C. Spalding (Eds.), . https://doi.org/10.1117/12.2027105

We discuss the design, implementation and performance of a novel platform for the production and optical control of ultra-low interfacial tension droplets in the 1-10 micron regime. A custom-designed, integrated microfluidic system allows the product... Read More about An optical platform for the production, trapping, manipulation and visualization of ultra-low interfacial tension emulsion droplets.