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A Non-Functional Carbon Dioxide-Mediated Post-Translational Modification on Nucleoside Diphosphate Kinase of Arabidopsis thaliana (2024)
Journal Article
Gannon, H. G., Riaz-Bradley, A., & Cann, M. J. (2024). A Non-Functional Carbon Dioxide-Mediated Post-Translational Modification on Nucleoside Diphosphate Kinase of Arabidopsis thaliana. International Journal of Molecular Sciences, 25(2), Article 898. https://doi.org/10.3390/ijms25020898

The carbamate post-translational modification (PTM), formed by the nucleophilic attack of carbon dioxide by a dissociated lysine epsilon-amino group, is proposed as a widespread mechanism for sensing this biologically important bioactive gas. Here, w... Read More about A Non-Functional Carbon Dioxide-Mediated Post-Translational Modification on Nucleoside Diphosphate Kinase of Arabidopsis thaliana.

Near atmospheric carbon dioxide activates plant ubiquitin cross-linking (2023)
Journal Article
Gannon, H. G., & Cann, M. J. (2023). Near atmospheric carbon dioxide activates plant ubiquitin cross-linking. https://doi.org/10.1016/j.bbadva.2023.100096

Background Identifying CO2-binding proteins is vital for our knowledge of CO2-regulated molecular processes. The carbamate post-translational modification is a reversible CO2-mediated adduct that can form on neutral N-terminal α-amino or lysine ε-ami... Read More about Near atmospheric carbon dioxide activates plant ubiquitin cross-linking.

Allophycocyanin A is a carbon dioxide receptor in the cyanobacterial phycobilisome (2022)
Journal Article
Guillen-Garcia, A., Gibson, S., Jordan, C., Ramaswamy, V., Linthwaite, V., Bromley, E., …Cann, M. (2022). Allophycocyanin A is a carbon dioxide receptor in the cyanobacterial phycobilisome. Nature Communications, 13, Article 5289. https://doi.org/10.1038/s41467-022-32925-6

Light harvesting is fundamental for production of ATP and reducing equivalents for CO2 fixation during photosynthesis. However, electronic energy transfer (EET) through a photosystem can harm the photosynthetic apparatus when not balanced with CO2. H... Read More about Allophycocyanin A is a carbon dioxide receptor in the cyanobacterial phycobilisome.

Carbon Dioxide and the Carbamate Post-Translational Modification (2022)
Journal Article
Blake, L. I., & Cann, M. J. (2022). Carbon Dioxide and the Carbamate Post-Translational Modification. Frontiers in Molecular Biosciences, 9, Article 825706. https://doi.org/10.3389/fmolb.2022.825706

Carbon dioxide is essential for life. It is at the beginning of every life process as a substrate of photosynthesis. It is at the end of every life process as the product of post-mortem decay. Therefore, it is not surprising that this gas regulates s... Read More about Carbon Dioxide and the Carbamate Post-Translational Modification.

Ubiquitin is a carbon dioxide-binding protein (2021)
Journal Article
Linthwaite, V., Pawloski, W., Pegg, H., Townsend, P., Thomas, M., Brown, A., …Cann, M. (2021). Ubiquitin is a carbon dioxide-binding protein. Science Advances, 7(39), Article eabi5507. https://doi.org/10.1126/sciadv.abi5507

The identification of CO2-binding proteins is crucial to understanding CO2-regulated molecular processes. CO2 can form a reversible posttranslational modification through carbamylation of neutral N-terminal -amino or lysine -amino groups. We have p... Read More about Ubiquitin is a carbon dioxide-binding protein.

Applying TADF Emitters in Bioimaging and Sensing—A Novel Approach Using Liposomes for Encapsulation and Cellular Uptake (2021)
Journal Article
Smith, P. O., Black, D. J., Pal, R., Avó, J., Dias, F. B., Linthwaite, V. L., …Pålsson, L. (2021). Applying TADF Emitters in Bioimaging and Sensing—A Novel Approach Using Liposomes for Encapsulation and Cellular Uptake. Frontiers in Chemistry, 9, Article 743928. https://doi.org/10.3389/fchem.2021.743928

A new method for facilitating the delivery, uptake and intracellular localisation of thermally activated delayed fluorescence (TADF) complexes was developed. First, confinement of TADF complexes in liposomes was demonstrated, which were subsequently... Read More about Applying TADF Emitters in Bioimaging and Sensing—A Novel Approach Using Liposomes for Encapsulation and Cellular Uptake.

A methodology for carbamate post-translational modification discovery and its application in Escherichia coli (2021)
Journal Article
Linthwaite, V. L., & Cann, M. J. (2021). A methodology for carbamate post-translational modification discovery and its application in Escherichia coli. Interface Focus, 11(2), Article 20200028. https://doi.org/10.1098/rsfs.2020.0028

Carbon dioxide can influence cell phenotypes through the modulation of signalling pathways. CO2 regulates cellular processes as diverse as metabolism, cellular homeostasis, chemosensing and pathogenesis. This diversity of regulated processes suggests... Read More about A methodology for carbamate post-translational modification discovery and its application in Escherichia coli.

Opposing modulation of Cx26 gap junctions and hemichannels by CO2 (2020)
Journal Article
Nijjar, S., Maddison, D., Meigh, L., Wolf, E., Rodgers, T., Cann, M., & Dale, N. (2021). Opposing modulation of Cx26 gap junctions and hemichannels by CO2. The Journal of Physiology, 599(1), 103-118. https://doi.org/10.1113/jp280747

Cx26 hemichannels open in response to moderate elevations of CO2 (PCO2 55 mmHg) via a carbamylation reaction that depends on residues K125 and R104. Here we investigate the action of CO2 on Cx26 gap junctions. Using a dye transfer assay, we found tha... Read More about Opposing modulation of Cx26 gap junctions and hemichannels by CO2.

A DNA-binding bromodomain-containing protein interacts with and reduces Rx1-mediated immune response to Potato Virus X (2020)
Journal Article
Sukarta, O. C., Townsend, P. D., Llewelyn, A., Dixon, C. H., Slootweg, E. J., Pålsson, L., …Cann, M. J. (2020). A DNA-binding bromodomain-containing protein interacts with and reduces Rx1-mediated immune response to Potato Virus X. Plant communications, 1(4), Article 100086. https://doi.org/10.1016/j.xplc.2020.100086

Plant NLR proteins enable the immune system to recognise and respond to pathogen attack. An early consequence of immune activation is transcriptional reprogramming. Some NLRs have been shown to act in the nucleus and interact with transcription facto... Read More about A DNA-binding bromodomain-containing protein interacts with and reduces Rx1-mediated immune response to Potato Virus X.

Distinct Roles of Non-Overlapping Surface Regions of the Coiled-Coil Domain in the Potato Immune Receptor Rx1 (2018)
Journal Article
Slootweg, E. J., Spiridon, L. N., Martin, E. C., Tameling, W. I., Townsend, P. D., Pomp, R., …Goverse, A. (2018). Distinct Roles of Non-Overlapping Surface Regions of the Coiled-Coil Domain in the Potato Immune Receptor Rx1. Plant Physiology, 178(3), 1310-1331. https://doi.org/10.1104/pp.18.00603

The intracellular immune receptor Rx1 of potato (Solanum tuberosum), which confers effector-triggered immunity to Potato virus X, consists of a central nucleotide-binding domain (NB-ARC) flanked by a carboxyl-terminal leucine-rich repeat (LRR) domain... Read More about Distinct Roles of Non-Overlapping Surface Regions of the Coiled-Coil Domain in the Potato Immune Receptor Rx1.

The identification of carbon dioxide mediated protein post-translational modifications (2018)
Journal Article
Linthwaite, V., Janus, J., Brown, A., Wong-Pascua, D., O’Donoghue, A., Porter, A., …Cann, M. (2018). The identification of carbon dioxide mediated protein post-translational modifications. Nature Communications, 9, Article 3092. https://doi.org/10.1038/s41467-018-05475-z

Carbon dioxide is vital to the chemistry of life processes including metabolism, cellular homoeostasis, and pathogenesis. CO2 is generally unreactive but can combine with neutral amines to form carbamates on proteins under physiological conditions. T... Read More about The identification of carbon dioxide mediated protein post-translational modifications.

The intracellular immune receptor Rx1 regulates the DNA-binding activity of a Golden2-like transcription factor (2017)
Journal Article
Townsend, P., Dixon, C., Slootweg, E., Sukarta, O., Yang, A., Hughes, T., …Cann, M. (2018). The intracellular immune receptor Rx1 regulates the DNA-binding activity of a Golden2-like transcription factor. Journal of Biological Chemistry, 293(9), 3218-3233. https://doi.org/10.1074/jbc.ra117.000485

Plant NLR proteins enable the immune system to recognise and respond to pathogen attack. An early consequence of immune activation is transcriptional reprogramming and some NLRs have been shown to act in the nucleus and interact with transcription fa... Read More about The intracellular immune receptor Rx1 regulates the DNA-binding activity of a Golden2-like transcription factor.

Hypercapnia modulates cAMP signalling and cystic fibrosis transmembrane conductance regulator-dependent anion and fluid secretion in airway epithelia (2015)
Journal Article
Turner, M., Saint-Crib, V., Patel, W., Ibrahim, S., Verdon, B., Ward, C., …Gray, M. (2016). Hypercapnia modulates cAMP signalling and cystic fibrosis transmembrane conductance regulator-dependent anion and fluid secretion in airway epithelia. The Journal of Physiology, 594(6), 1643-1661. https://doi.org/10.1113/jp271309

Hypercapnia is clinically defined as an arterial blood partial pressure of CO2 of above 40 mmHg and is a feature of chronic lung disease. In previous studies we have demonstrated that hypercapnia modulates agonist-stimulated cAMP levels through effec... Read More about Hypercapnia modulates cAMP signalling and cystic fibrosis transmembrane conductance regulator-dependent anion and fluid secretion in airway epithelia.

The tomato Nucleotide-Binding Leucine-Rich Repeat (NLR) Immune Receptor I-2 couples DNA-Binding to Nucleotide-Binding Domain Nucleotide Exchange (2015)
Journal Article
Fenyk, S., Dixon, C., Kittens, W., Townsend, P., Sharpies, G., Pålsson, L., …Cann, M. (2016). The tomato Nucleotide-Binding Leucine-Rich Repeat (NLR) Immune Receptor I-2 couples DNA-Binding to Nucleotide-Binding Domain Nucleotide Exchange. Journal of Biological Chemistry, 291(3), 1137-1147. https://doi.org/10.1074/jbc.m115.698589

Plant nucleotide-binding leucine-rich repeat (NLR) proteins enable plants to recognise and respond to pathogen attack. Previously, we demonstrated that the Rx1 NLR of potato is able to bind and bend DNA in vitro. DNA binding in situ requires its genu... Read More about The tomato Nucleotide-Binding Leucine-Rich Repeat (NLR) Immune Receptor I-2 couples DNA-Binding to Nucleotide-Binding Domain Nucleotide Exchange.

Multi-scale Approaches to Dynamical Transmission of Protein Allostery (2015)
Conference Proceeding
Townsend, P., Rodgers, T., Pohl, E., Wilson, M., Cann, M., & McLeish, T. (2015). Multi-scale Approaches to Dynamical Transmission of Protein Allostery. In L. Olivares-Quiroz, O. Guzmán-López, & H. E. Jardón-Valadez (Eds.), Physical Biology of Proteins and Peptides: Theory, Experiment, and Simulation (141-152). https://doi.org/10.1007/978-3-319-21687-4_8

We review the idea that allosteric interactions can be transmitted not by structural switching but by the more subtle route of modulation of the amplitude of thermally-activated global dynamical modes in allosteric proteins. The effect is naturally a... Read More about Multi-scale Approaches to Dynamical Transmission of Protein Allostery.

Dynamic Transmission of Protein Allostery without Structural Change: Spatial Pathways or Global Modes? (2015)
Journal Article
McLeish, T., Cann, M., & Rodgers, T. (2015). Dynamic Transmission of Protein Allostery without Structural Change: Spatial Pathways or Global Modes?. Biophysical Journal, 109(6), 1240-1250. https://doi.org/10.1016/j.bpj.2015.08.009

We examine the contrast between mechanisms for allosteric signaling that involve structural change, and those that do not, from the perspective of allosteric pathways. In particular we treat in detail the case of fluctuation-allostery by which amplit... Read More about Dynamic Transmission of Protein Allostery without Structural Change: Spatial Pathways or Global Modes?.

The Potato Nucleotide-Binding Leucine-Rich Repeat (NLR) Immune Receptor Rx1 is a Pathogen Dependent DNA-Deforming Protein (2015)
Journal Article
Fenyk, S., Townsend, P. D., Dixon, C. H., Spies, G. B., de San Eustaquio Campillo, A., Slootweg, E. J., …Cann, M. J. (2015). The Potato Nucleotide-Binding Leucine-Rich Repeat (NLR) Immune Receptor Rx1 is a Pathogen Dependent DNA-Deforming Protein. Journal of Biological Chemistry, 290(41), 24945-24960. https://doi.org/10.1074/jbc.m115.672121

Plant NLR proteins enable cells to respond to pathogen attack. Several NLRs act in the nucleus, however, conserved nuclear targets that support their role in immunity are unknown. Previously we noted a structural homology between the NB domain of NLR... Read More about The Potato Nucleotide-Binding Leucine-Rich Repeat (NLR) Immune Receptor Rx1 is a Pathogen Dependent DNA-Deforming Protein.

Ecto-5'-Nucleotidase, Adenosine and Transmembrane Adenylyl Cyclase Signalling Regulate Basal Carotid Body Chemoafferent Outflow and Establish the Sensitivity to Hypercapnia. (2015)
Book Chapter
Holmes, A., Nunes, A., Cann, M., & Kumar, P. (2015). Ecto-5'-Nucleotidase, Adenosine and Transmembrane Adenylyl Cyclase Signalling Regulate Basal Carotid Body Chemoafferent Outflow and Establish the Sensitivity to Hypercapnia. In C. Peers, P. Kumar, C. Wyatt, E. Gauda, C. A. Nurse, & N. Prabhakar (Eds.), Arterial Chemoreceptors in Physiology and Pathophysiology (279-289). Springer. https://doi.org/10.1007/978-3-319-18440-1_32

Carotid body (CB) stimulation by hypercapnia causes a reflex increase in ventilation and, along with the central chemoreceptors, this prevents a potentially lethal systemic acidosis. Control over the CB chemoafferent output during normocapnia and hyp... Read More about Ecto-5'-Nucleotidase, Adenosine and Transmembrane Adenylyl Cyclase Signalling Regulate Basal Carotid Body Chemoafferent Outflow and Establish the Sensitivity to Hypercapnia..

The role of protein-ligand contacts in allosteric regulation of the Escherichia coli Catabolite Activator Protein (2015)
Journal Article
Townsend, P., Rodgers, T., Glover, L., Korhonen, H., Richards, S., Colwell, L., …Cann, M. (2015). The role of protein-ligand contacts in allosteric regulation of the Escherichia coli Catabolite Activator Protein. Journal of Biological Chemistry, 290(36), 22225-22235. https://doi.org/10.1074/jbc.m115.669267

Allostery is a fundamental process by which ligand binding to a protein alters its activity at a distant site. Both experimental and theoretical evidence demonstrate that allostery can be communicated through altered slow relaxation protein dynamics... Read More about The role of protein-ligand contacts in allosteric regulation of the Escherichia coli Catabolite Activator Protein.

Global low-frequency motions in protein allostery: CAP as a model system (2015)
Journal Article
Townsend, P., Rogers, T., Pohl, E., Wilson, M., McLeish, T., & Cann, M. (2015). Global low-frequency motions in protein allostery: CAP as a model system. Biophysical Reviews, 7(2), 175-182. https://doi.org/10.1007/s12551-015-0163-9

Allostery is a fundamental process by which ligand binding to a protein alters its activity at a distant site. There is considerable evidence that allosteric cooperativity can be communicated by the modulation of protein dynamics without conformation... Read More about Global low-frequency motions in protein allostery: CAP as a model system.

The crystal structures of apo and cAMP-bound GlxR from Corynebacterium glutamicum reveal structural and dynamic changes upon cAMP binding in CRP/FNR family transcription factors (2014)
Journal Article
Townsend, P., Jungwirth, B., Pojer, P., Bußmann, M., Money, V., Cole, S., …Pohl, E. (2014). The crystal structures of apo and cAMP-bound GlxR from Corynebacterium glutamicum reveal structural and dynamic changes upon cAMP binding in CRP/FNR family transcription factors. PLoS ONE, 9(12), Article e113265. https://doi.org/10.1371/journal.pone.0113265

The cyclic AMP-dependent transcriptional regulator GlxR from Corynebacterium glutamicum is a member of the super-family of CRP/FNR (cyclic AMP receptor protein/fumarate and nitrate reduction regulator) transcriptional regulators that play central rol... Read More about The crystal structures of apo and cAMP-bound GlxR from Corynebacterium glutamicum reveal structural and dynamic changes upon cAMP binding in CRP/FNR family transcription factors.

CO2 directly modulates connexin 26 by formation of carbamate bridges between subunits (2013)
Journal Article
Meigh, L., Greenhalgh, S., Rodgers, T., Cann, M., Roper, D., & Dale, N. (2013). CO2 directly modulates connexin 26 by formation of carbamate bridges between subunits. eLife, 2, https://doi.org/10.7554/elife.01213

Homeostatic regulation of the partial pressure of CO2 (PCO2) is vital for life. Sensing of pH has been proposed as a sufficient proxy for determination of PCO2 and direct CO2-sensing largely discounted. Here we show that connexin 26 (Cx26) hemichanne... Read More about CO2 directly modulates connexin 26 by formation of carbamate bridges between subunits.

Modulation of Global Low-Frequency Motions Underlies Allosteric Regulation: Demonstration in CRP/FNR Family Transcription Factors (2013)
Journal Article
Rodgers, T., Townsend, P., Burnell, D., Jones, M., Richards, S., McLeish, T., …Cann, M. (2013). Modulation of Global Low-Frequency Motions Underlies Allosteric Regulation: Demonstration in CRP/FNR Family Transcription Factors. PLoS Biology, 11(9), Article e1001651. https://doi.org/10.1371/journal.pbio.1001651

Allostery is a fundamental process by which ligand binding to a protein alters its activity at a distinct site. There is growing evidence that allosteric cooperativity can be communicated by modulation of protein dynamics without conformational chang... Read More about Modulation of Global Low-Frequency Motions Underlies Allosteric Regulation: Demonstration in CRP/FNR Family Transcription Factors.

Elevated Carbon Dioxide Blunts Mammalian cAMP Signaling Dependent on Inositol 1,4,5-Triphosphate Receptor-mediated Ca2+ Release (2012)
Journal Article
Cook, Z., Gray, M., & Cann, M. (2012). Elevated Carbon Dioxide Blunts Mammalian cAMP Signaling Dependent on Inositol 1,4,5-Triphosphate Receptor-mediated Ca2+ Release. Journal of Biological Chemistry, 287(31), 26291-26301. https://doi.org/10.1074/jbc.m112.349191

Elevated CO2 is generally detrimental to animal cells, suggesting an interaction with core processes in cell biology. We demonstrate that elevated CO2 blunts G protein-activated cAMP signaling. The effect of CO2 is independent of changes in intracell... Read More about Elevated Carbon Dioxide Blunts Mammalian cAMP Signaling Dependent on Inositol 1,4,5-Triphosphate Receptor-mediated Ca2+ Release.

A Nucleotide Phosphatase Activity in the Nucleotide Binding Domain of an Orphan Resistance Protein from Rice (2012)
Journal Article
Fenyk, S., de San Eustaquio Campillo, A., Pohl, E., Hussey, P., & Cann, M. (2012). A Nucleotide Phosphatase Activity in the Nucleotide Binding Domain of an Orphan Resistance Protein from Rice. Journal of Biological Chemistry, 287(6), 4023-4032. https://doi.org/10.1074/jbc.m111.314450

Plant resistance proteins (R-proteins) are key components of the plant immune system activated in response to a plethora of different pathogens. R-proteins are P-loop NTPase superfamily members, and current models describe their main function as ATPa... Read More about A Nucleotide Phosphatase Activity in the Nucleotide Binding Domain of an Orphan Resistance Protein from Rice.

The Pseudomonas aeruginosa Chp chemosensory system regulates intracellular cAMP levels by modulating adenylate cyclase activity (2010)
Journal Article
Fulcher, N., Holliday, P., Klem, E., Cann, M., & Wolfgang, M. (2010). The Pseudomonas aeruginosa Chp chemosensory system regulates intracellular cAMP levels by modulating adenylate cyclase activity. Molecular Microbiology, 76(4), 889-904. https://doi.org/10.1111/j.1365-2958.2010.07135.x

Multiple virulence systems in the opportunistic pathogen Pseudomonas aeruginosa are regulated by the second messenger signalling molecule adenosine 3′, 5′-cyclic monophosphate (cAMP). Production of cAMP by the putative adenylate cyclase enzyme CyaB r... Read More about The Pseudomonas aeruginosa Chp chemosensory system regulates intracellular cAMP levels by modulating adenylate cyclase activity.

Stimulation of Mammalian G-protein-responsive Adenylyl Cyclases by Carbon Dioxide (2009)
Journal Article
Townsend, P., Holliday, P., Fenyk, S., Hess, K., Gray, M., Hodgson, D., & Cann, M. (2009). Stimulation of Mammalian G-protein-responsive Adenylyl Cyclases by Carbon Dioxide. Journal of Biological Chemistry, 284(2), 784-791. https://doi.org/10.1074/jbc.m807239200

Carbon dioxide is fundamental to the physiology of all organisms. There is considerable interest in the precise molecular mechanisms that organisms use to directly sense CO2. Here we demonstrate that a mammalian recombinant G-protein-activated adenyl... Read More about Stimulation of Mammalian G-protein-responsive Adenylyl Cyclases by Carbon Dioxide.

Sodium regulation of GAF domain function (2007)
Journal Article
Cann, M. (2007). Sodium regulation of GAF domain function. Biochemical Society Transactions, 35(5), 1032-1034. https://doi.org/10.1042/bst0351032

Cyclic nucleotide PDEs (phosphodiesterases) regulate cellular levels of cAMP and cGMP by controlling the rate of degradation. Several mammalian PDE isoforms possess N-terminal GAF (found in cGMP PDEs, Anabaena adenylate cyclases and Escherichia coli... Read More about Sodium regulation of GAF domain function.

Synthesis of 5'-Amino-5'-deoxyguanosine-5'-N-phosphoramidate and its Enzymatic Incorporation at the 5'-Termini of RNA Molecules (2007)
Journal Article
Williamson, D., Cann, M., & Hodgson, D. (2007). Synthesis of 5'-Amino-5'-deoxyguanosine-5'-N-phosphoramidate and its Enzymatic Incorporation at the 5'-Termini of RNA Molecules. Chemical Communications, 2007(47), 5096-5098. https://doi.org/10.1039/b712066d

5-Amino-5-deoxyguanosine-5-N-phosphoramidate (GNHP) was synthesized in four steps from guanosine and was found to initiate T7 RNAP-promoted transcriptions to afford 5-H2N-RNA that can be conjugated to activated esters.

A subset of GAF domains are evolutionarily conserved sodium sensors (2007)
Journal Article
Cann, M. (2007). A subset of GAF domains are evolutionarily conserved sodium sensors. Molecular Microbiology, 64(2), 461-472. https://doi.org/10.1111/j.1365-2958.2007.05669.x

Most organisms maintain a transmembrane sodium gradient for cell function. Despite the importance of Na+ in physiology, no directly Na+-responsive signalling molecules are known. The CyaB1 and CyaB2 adenylyl cyclases of the cyanobacterium Anabaena PC... Read More about A subset of GAF domains are evolutionarily conserved sodium sensors.

A europium complex that selectively stains nucleoli of cells (2006)
Journal Article
Yu, J., Parker, D., Pal, R., Poole, R., & Cann, M. (2006). A europium complex that selectively stains nucleoli of cells. Journal of the American Chemical Society, 128(7), 2294-2299. https://doi.org/10.1021/ja056303g

A europium complex selectively staining the nucleolus of NIH 3T3, HeLa, and HDF cells is reported. This complex possesses not only the advantage of the long lifetime of europium emission (0.3 ms), but also a chromophore that allows excitation at a re... Read More about A europium complex that selectively stains nucleoli of cells.

Regulation of prokaryotic adenylyl cyclases by CO₂ (2006)
Journal Article
Hammer, A., Hodgson, D., & Cann, M. (2006). Regulation of prokaryotic adenylyl cyclases by CO₂. Biochemical Journal, 396(2), 215-218. https://doi.org/10.1042/bj20060372

The Slr1991 adenylyl cyclase of the model prokaroyte Synechocystis PCC 6803 was stimulated 2-fold at 20 mM total Ci (inorganic carbon) atpH 7.5 through an increase in kcat.Adose response demonstrated an EC50 of 52.7 mMtotal Ci at pH 6.5. Slr1991 aden... Read More about Regulation of prokaryotic adenylyl cyclases by CO₂.

Synthesis and characterisation of highly emissive and kinetically stable lanthanide complexes suitable for usage 'in cellulo' (2005)
Journal Article
Poole, R., Bobba, G., Cann, M., Frias, J., Parker, D., & Peacock, R. (2005). Synthesis and characterisation of highly emissive and kinetically stable lanthanide complexes suitable for usage 'in cellulo'. Organic and Biomolecular Chemistry, 3(6), 1013-1024. https://doi.org/10.1039/b418964g

The synthesis and photophysical characterisation are reported of a series of cationic, neutral and anionic europium and terbium complexes based on structurally related, nonadentate ligands based on the cyclen macrocycle. Each complex incorporates a t... Read More about Synthesis and characterisation of highly emissive and kinetically stable lanthanide complexes suitable for usage 'in cellulo'.

Bicarbonate stimulated adenylyl cyclases (2004)
Journal Article
Cann, M. (2004). Bicarbonate stimulated adenylyl cyclases. IUBMB Life, 56(9), 529-534. https://doi.org/10.1080/15216540400013861

Bicarbonate ion is fundamental to the biology of all living organisms. HCO(3)(-) is vital to such diverse physiological processes as carbon fixation, cellular homeostasis, sperm maturation, and nucleotide synthesis. A defined subset of adenylyl cycla... Read More about Bicarbonate stimulated adenylyl cyclases.

Design, synthesis and evaluation of ratiometric probes for hydrogencarbonate based on europium emission (2004)
Journal Article
Bretonniere, Y., Cann, M., Parker, D., & Slater, R. (2004). Design, synthesis and evaluation of ratiometric probes for hydrogencarbonate based on europium emission. Organic and Biomolecular Chemistry, 2(11), 1624-1632. https://doi.org/10.1039/b400734b

A series of cationic, zwitterionic and anionic macrocyclic europium complexes has been prepared incorporating a N or C- linked acridone chromophore that allows sensitisation of Eu emission following excitation at 390-410nm. Each of these complexes se... Read More about Design, synthesis and evaluation of ratiometric probes for hydrogencarbonate based on europium emission.

Luminescent nonacoordinate cationic lanthanide complexes as potential cellular imaging and reactive probes (2003)
Journal Article
Frias, J., Bobba, G., Cann, M., Hutchison, C., & Parker, D. (2003). Luminescent nonacoordinate cationic lanthanide complexes as potential cellular imaging and reactive probes. Organic and Biomolecular Chemistry, 1(6), 905-907

Nonacoordinate Delta- and Lambda-Eu and Tb complexes have been tested as imaging and reactive probes in mouse fibroblast (NIH 3T3) cells. The uptake of these complexes by the cells was assessed by fluorescence microscopy. Complex-induced DNA damage w... Read More about Luminescent nonacoordinate cationic lanthanide complexes as potential cellular imaging and reactive probes.

A defined subset of adenylyl cyclases is regulated by bicarbonate ion (2003)
Journal Article
Cann, M., Hammer, A., Zhou, J., & Kanacher, T. (2003). A defined subset of adenylyl cyclases is regulated by bicarbonate ion. Journal of Biological Chemistry, 278(37), 35033-35038. https://doi.org/10.1074/jbc.m303025200

The molecular basis by which organisms detect and respond to fluctuations in inorganic carbon is not known. The cyaB1 gene of the cyanobacterium Anabaena sp. PCC7120 codes for a multidomain protein with a C-terminal class III adenylyl cyclase catalys... Read More about A defined subset of adenylyl cyclases is regulated by bicarbonate ion.

Ratiometric probes for hydrogencarbonate analysis in intracellular or extracellular environments using europium luminescence (2002)
Journal Article
Bretonnière, Y., Cann, M., Parker, D., & Slater, R. (2002). Ratiometric probes for hydrogencarbonate analysis in intracellular or extracellular environments using europium luminescence. Chemical Communications, 2002(17), 1930-1931. https://doi.org/10.1039/b206286k

A series of six, cationic, zwitterionic and anionic Eu complexes has been examined for the analysis of hydrogencarbonate concentration in the intracellular and extracellular ranges; an anionic complex incorporating three glutarate residues and a sens... Read More about Ratiometric probes for hydrogencarbonate analysis in intracellular or extracellular environments using europium luminescence.

Soluble adenylyl cyclase as an evolutionarily conserved bicarbonate sensor. (2000)
Journal Article
Chen, Y., Cann, M., Litvin, T., Iourgenko, V., Sinclair, M., Levin, L., & Buck, J. (2000). Soluble adenylyl cyclase as an evolutionarily conserved bicarbonate sensor. Science, 289(5479), 625-628. https://doi.org/10.1126/science.289.5479.625

Spermatozoa undergo a poorly understood activation process induced by bicarbonate and mediated by cyclic adenosine 3′,5′-monophosphate (cAMP). It has been assumed that bicarbonate mediates its effects through changes in intracellular pH or membrane p... Read More about Soluble adenylyl cyclase as an evolutionarily conserved bicarbonate sensor..

A new family of adenylyl cyclase genes in the male germline of Drosophila melanogaster. (2000)
Journal Article
Cann, M., Chung, E., & Levin, L. (2000). A new family of adenylyl cyclase genes in the male germline of Drosophila melanogaster. Development Genes and Evolution, 210(4), 200-206. https://doi.org/10.1007/s004270050304

We describe the cloning and characterization of a new gene family of adenylyl cyclase related genes in Drosophila. The five adenylyl cyclase-like genes that define this family are clearly distinct from previously known adenylyl cyclases. One member f... Read More about A new family of adenylyl cyclase genes in the male germline of Drosophila melanogaster..

Restricted expression of a truncated adenylyl cyclase in the cephalic furrow of Drosophila melanogaster (2000)
Journal Article
Cann, M., & Levin, L. (2000). Restricted expression of a truncated adenylyl cyclase in the cephalic furrow of Drosophila melanogaster. Development Genes and Evolution, 210(1), 34-40. https://doi.org/10.1007/pl00008186

We have identified a novel isoform of adenylyl cyclase, DAC78C, in Drosophila melanogaster that encodes two structurally distinct proteins in a developmentally restricted manner. The protein corresponding to one transcript is potently activated by G... Read More about Restricted expression of a truncated adenylyl cyclase in the cephalic furrow of Drosophila melanogaster.

Cytosolic adenylyl cyclase defines a unique signaling molecule in mammals. (1999)
Journal Article
Buck, J., Sinclair, M., Schapal, L., Cann, M., & Levin, L. (1999). Cytosolic adenylyl cyclase defines a unique signaling molecule in mammals. Proceedings of the National Academy of Sciences, 96(1), 79-84. https://doi.org/10.1073/pnas.96.1.79

Mammals have nine differentially regulated isoforms of G protein-responsive transmembrane-spanning adenylyl cyclases. We now describe the existence of a distinct class of mammalian adenylyl cyclase that is soluble and insensitive to G protein or Fors... Read More about Cytosolic adenylyl cyclase defines a unique signaling molecule in mammals..

Cloning and characterization of a Drosophila adenylyl cyclase homologous to mammalian type IX. (1997)
Journal Article
Iourgenko, V., Kliot, B., Cann, M., & Levin, L. (1997). Cloning and characterization of a Drosophila adenylyl cyclase homologous to mammalian type IX. FEBS Letters, 413(11), 104-108. https://doi.org/10.1016/s0014-5793%2897%2900891-0

A novel Drosophila adenylyl cyclase (AC) was identified by PCR using degenerate primers specific for the known metazoan ACs. The full-length cDNA predicts a protein displaying significant sequence homology with mammalian Type IX AC (AC9). The abundan... Read More about Cloning and characterization of a Drosophila adenylyl cyclase homologous to mammalian type IX..