P.D. Townsend
The intracellular immune receptor Rx1 regulates the DNA-binding activity of a Golden2-like transcription factor
Townsend, P.D.; Dixon, C.H.; Slootweg, E.S.; Sukarta, O.C.A.; Yang, A.W.H.; Hughes, T.R.; Sharples, G.J.; Palsson, L.-O.; Takken, F.L.W.; Goverse, A.; Cann, M.J.
Authors
C.H. Dixon
E.S. Slootweg
O.C.A. Sukarta
A.W.H. Yang
T.R. Hughes
Dr Gary Sharples gary.sharples@durham.ac.uk
Associate Professor
Dr Lars- Palsson lars-olof.palsson@durham.ac.uk
Associate Professor
F.L.W. Takken
A. Goverse
Professor Martin Cann m.j.cann@durham.ac.uk
Professor
Abstract
Plant NLR proteins enable the immune system to recognise and respond to pathogen attack. An early consequence of immune activation is transcriptional reprogramming and some NLRs have been shown to act in the nucleus and interact with transcription factors. The Rx1 NLR protein of potato is further able to bind and distort double-stranded DNA. However, Rx1 host targets that support a role for Rx1 in transcriptional reprogramming at DNA are unknown. Here we report a functional interaction between Rx1 and NbGlk1, a Golden2- like transcription factor. Rx1 binds to NbGlk1 in vitro and in planta. NbGlk1 binds to known Golden2-like consensus DNA sequences. Rx1 reduces the binding affinity of NbGlk1 for DNA in vitro. NbGlk1 activates cellular responses to potato virus X, whereas Rx1 associates with NbGlk1 and prevents its assembly on DNA in planta unless activated by PVX. This study provides new mechanistic insight into how an NLR can co-ordinate an immune signalling response at DNA following pathogen perception.
Citation
Townsend, P., Dixon, C., Slootweg, E., Sukarta, O., Yang, A., Hughes, T., …Cann, M. (2018). The intracellular immune receptor Rx1 regulates the DNA-binding activity of a Golden2-like transcription factor. Journal of Biological Chemistry, 293(9), 3218-3233. https://doi.org/10.1074/jbc.ra117.000485
Journal Article Type | Article |
---|---|
Acceptance Date | Dec 7, 2017 |
Online Publication Date | Dec 7, 2017 |
Publication Date | Mar 1, 2018 |
Deposit Date | Jun 7, 2017 |
Publicly Available Date | Mar 2, 2018 |
Journal | Journal of Biological Chemistry |
Print ISSN | 0021-9258 |
Electronic ISSN | 1083-351X |
Publisher | American Society for Biochemistry and Molecular Biology |
Peer Reviewed | Peer Reviewed |
Volume | 293 |
Issue | 9 |
Pages | 3218-3233 |
DOI | https://doi.org/10.1074/jbc.ra117.000485 |
Public URL | https://durham-repository.worktribe.com/output/1385453 |
Files
Accepted Journal Article
(10.3 Mb)
PDF
Copyright Statement
This article is published under a Creative Commons CC-BY license.
Published Journal Article
(3.6 Mb)
PDF
Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/
You might also like
Allophycocyanin A is a carbon dioxide receptor in the cyanobacterial phycobilisome
(2022)
Journal Article
Modulated Fluorescence in LB Films Based on DADQs—A Potential Sensing Surface?
(2022)
Journal Article
TADF Dye-Loaded Nanoparticles for Fluorescence Live-Cell Imaging
(2020)
Journal Article
A bacteriophage mimic of the bacterial nucleoid-associated protein Fis
(2020)
Journal Article
Downloadable Citations
About Durham Research Online (DRO)
Administrator e-mail: dro.admin@durham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2025
Advanced Search