Synergistic interplay of redox homeostasis and polysaccharide synthesis promotes cotton fiber elongation

Tian, Xuehan; Ji, Mengyuan; You, Jiaqi; Zhang, Yuqi; Lindsey, Keith; Zhang, Xianlong; Tu, Lili; Wang, Maojun

Abstract

Cell polarity is the foundation of cell development and tissue morphogenesis. The investigation of polarized growth provides opportunities to gain profound insights into morphogenesis and tissue functionality in organisms. Currently, there are still many mysteries surrounding the mechanisms that regulate polarized cell growth. Cotton fiber cells serve as an excellent model for studying polarized growth, and provide important clues for unraveling the molecular mechanisms, signaling pathways, and regulatory networks of polarized growth. In this study, we characterized two functional genes, GhMDHAR1A /D and GhDHAR2A /D with predominant expression during fiber elongation. Loss of function of both genes contributed to a significant increase in fiber length. Transcriptomic data revealed up-regulated expression of antioxidant genes in CRISPR mutant lines, along with delayed expression of secondary wall-related genes and temporally prolonged expression of primary wall-related genes. Experimental evidence demonstrated that the increase in GSH content and glutathione peroxidase (GPX) enzyme activity led to enhanced total antioxidant capacity (T-AOC), resulting in reduced H O levels, which contributed to the extension of fiber elongation stage in CRISPR mutant lines. Moreover, the increased polysaccharide synthesis in CRISPR mutant lines was found to provide an abundant supply of raw materials for fiber cell wall elongation, suggesting that synergistic interplay between redox homeostasis and polysaccharide synthesis in fiber cells may facilitate cell wall remodeling and fiber elongation. This study provides valuable insights for deciphering the mechanisms of cell polarized growth and improving cotton fiber quality. [Abstract copyright: © 2024 Society for Experimental Biology and John Wiley & Sons Ltd.]

Citation

Tian, X., Ji, M., You, J., Zhang, Y., Lindsey, K., Zhang, X., Tu, L., & Wang, M. (2024). Synergistic interplay of redox homeostasis and polysaccharide synthesis promotes cotton fiber elongation. The Plant Journal, 118(2), 405-422. https://doi.org/10.1111/tpj.16615

• Journal Article Type: Article
• Acceptance Date: Dec 19, 2023
• Online Publication Date: Jan 1, 2024
• Publication Date: 2024-04
• Deposit Date: Jan 9, 2024
• Journal: The Plant Journal
• Print ISSN: 0960-7412
• Electronic ISSN: 1365-313X
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 118
• Issue: 2
• Pages: 405-422
• DOI: https://doi.org/10.1111/tpj.16615
• Keywords: ascorbic acid, polarized growth, MDHAR, redox homeostasis, DHAR, polysaccharide synthesis, cotton fiber
• Public URL: https://durham-repository.worktribe.com/output/2116730