Huaguo Zhu
The complex hexaploid oil‐Camellia genome traces back its phylogenomic history and multi‐omics analysis of Camellia oil biosynthesis
Zhu, Huaguo; Wang, Fuqiu; Xu, Zhongping; Wang, Guanying; Hu, Lisong; Cheng, Junyong; Ge, Xianhong; Liu, Jinxuan; Chen, Wei; Li, Qiang; Xue, Fei; Liu, Feng; Li, Wenying; Wu, Lan; Cheng, Xinqi; Tang, Xinxin; Yang, Chaochen; Lindsey, Keith; Zhang, Xianlong; Ding, Fang; Hu, Haiyan; Hu, Xiaoming; Jin, Shuangxia
Authors
Fuqiu Wang
Zhongping Xu
Guanying Wang
Lisong Hu
Junyong Cheng
Xianhong Ge
Jinxuan Liu
Wei Chen
Qiang Li
Fei Xue
Feng Liu
Wenying Li
Lan Wu
Xinqi Cheng
Xinxin Tang
Chaochen Yang
Professor Keith Lindsey keith.lindsey@durham.ac.uk
Professor
Xianlong Zhang
Fang Ding
Haiyan Hu
Xiaoming Hu
Shuangxia Jin
Abstract
Summary: Oil‐Camellia (Camellia oleifera), belonging to the Theaceae family Camellia, is an important woody edible oil tree species. The Camellia oil in its mature seed kernels, mainly consists of more than 90% unsaturated fatty acids, tea polyphenols, flavonoids, squalene and other active substances, which is one of the best quality edible vegetable oils in the world. However, genetic research and molecular breeding on oil‐Camellia are challenging due to its complex genetic background. Here, we successfully report a chromosome‐scale genome assembly for a hexaploid oil‐Camellia cultivar Changlin40. This assembly contains 8.80 Gb genomic sequences with scaffold N50 of 180.0 Mb and 45 pseudochromosomes comprising 15 homologous groups with three members each, which contain 135 868 genes with an average length of 3936 bp. Referring to the diploid genome, intragenomic and intergenomic comparisons of synteny indicate homologous chromosomal similarity and changes. Moreover, comparative and evolutionary analyses reveal three rounds of whole‐genome duplication (WGD) events, as well as the possible diversification of hexaploid Changlin40 with diploid occurred approximately 9.06 million years ago (MYA). Furthermore, through the combination of genomics, transcriptomics and metabolomics approaches, a complex regulatory network was constructed and allows to identify potential key structural genes (SAD, FAD2 and FAD3) and transcription factors (AP2 and C2H2) that regulate the metabolism of Camellia oil, especially for unsaturated fatty acids biosynthesis. Overall, the genomic resource generated from this study has great potential to accelerate the research for the molecular biology and genetic improvement of hexaploid oil‐Camellia, as well as to understand polyploid genome evolution.
Citation
Zhu, H., Wang, F., Xu, Z., Wang, G., Hu, L., Cheng, J., …Jin, S. (2024). The complex hexaploid oil‐Camellia genome traces back its phylogenomic history and multi‐omics analysis of Camellia oil biosynthesis. Plant Biotechnology Journal, https://doi.org/10.1111/pbi.14412
Journal Article Type | Article |
---|---|
Acceptance Date | May 29, 2024 |
Online Publication Date | Jun 24, 2024 |
Publication Date | Jun 24, 2024 |
Deposit Date | Jul 3, 2024 |
Publicly Available Date | Jul 3, 2024 |
Journal | Plant Biotechnology Journal |
Print ISSN | 1467-7644 |
Publisher | Association of Applied Biologists |
Peer Reviewed | Peer Reviewed |
DOI | https://doi.org/10.1111/pbi.14412 |
Keywords | whole‐genome duplication, Oil‐Camellia (Camellia oleifera), chromosome‐scale genome assembly, hexaploid, camellia oil biosynthesis |
Public URL | https://durham-repository.worktribe.com/output/2512904 |
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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/
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