Evolution and subfunctionalization of CIPK6 homologous genes in regulating cotton drought resistance

Sun, Weinan; Xia, Linjie; Deng, Jinwu; Sun, Simin; Yue, Dandan; You, Jiaqi; Wang, Maojun; Jin, Shuangxia; Zhu, Longfu; Lindsey, Keith; Zhang, Xianlong; Yang, Xiyan

Abstract

The occurrence of whole-genome duplication or polyploidy may promote plant adaptability to harsh environments. Here, we clarify the evolutionary relationship of eight GhCIPK6 homologous genes in upland cotton (Gossypium hirsutum). Gene expression and interaction analyses indicate that GhCIPK6 homologous genes show significant functional changes after polyploidy. Among these, GhCIPK6D1 and GhCIPK6D3 are significantly up-regulated by drought stress. Functional studies reveal that high GhCIPK6D1 expression promotes cotton drought sensitivity, while GhCIPK6D3 expression promotes drought tolerance, indicating clear functional differentiation. Genetic and biochemical analyses confirm the synergistic negative and positive regulation of cotton drought resistance through GhCBL1A1-GhCIPK6D1 and GhCBL2A1-GhCIPK6D3, respectively, to regulate stomatal movement by controlling the directional flow of K+ in guard cells. These results reveal differentiated roles of GhCIPK6 homologous genes in response to drought stress in upland cotton following polyploidy. The work provides a different perspective for exploring the functionalization and subfunctionalization of duplicated genes in response to polyploidization.

Citation

Sun, W., Xia, L., Deng, J., Sun, S., Yue, D., You, J., Wang, M., Jin, S., Zhu, L., Lindsey, K., Zhang, X., & Yang, X. (2024). Evolution and subfunctionalization of CIPK6 homologous genes in regulating cotton drought resistance. Nature Communications, 15(1), Article 5733. https://doi.org/10.1038/s41467-024-50097-3

• Journal Article Type: Article
• Acceptance Date: Jun 28, 2024
• Online Publication Date: Jul 9, 2024
• Publication Date: Jul 9, 2024
• Deposit Date: Jul 18, 2024
• Publicly Available Date: Jul 18, 2024
• Journal: Nature Communications
• Electronic ISSN: 2041-1723
• Publisher: Nature Research
• Peer Reviewed: Peer Reviewed
• Volume: 15
• Issue: 1
• Article Number: 5733
• DOI: https://doi.org/10.1038/s41467-024-50097-3
• Public URL: https://durham-repository.worktribe.com/output/2528893