A predictive model for ethylene-mediated auxin and cytokinin patterning in the Arabidopsis root

Moore, Simon; Jervis, George; Topping, Jennifer; Chen, Chunli; Liu, Junli; Lindsey, Keith

Abstract

The interaction between auxin and cytokinin is important in many aspects of plant development. Experimental measurements of both auxin and cytokinin concentration and reporter gene expression clearly show the coexistence of auxin and cytokinin concentration patterning in Arabidopsis root development. However, in the context of crosstalk between auxin, cytokinin and ethylene, little is known about how auxin and cytokinin concentration patterns simultaneously emerge and how they regulate each other in the Arabidopsis root. This work utilizes a wide range of experimental observations to propose a mechanism for simultaneous patterning of auxin and cytokinin concentration. In addition to the regulatory relationships between auxin and cytokinin, the mechanism reveals that ethylene signalling is an important factor in achieving simultaneous auxin and cytokinin patterning, while also predicting other experimental observations. Combining the mechanism with a realistic in silico root model reproduces experimental observations of both auxin and cytokinin patterning. Predictions made by the mechanism can be compared with a variety of experimental observations, including those conducted by our group and other independent experiments reported by other groups. Examples of these predictions include patterning of auxin biosynthesis rate, PIN1 and PIN2 pattern changes in pin3, 4, 7 mutants, cytokinin patterning change in the pls mutant, PLS patterning, as well as various trends in different mutants. This research unravels a plausible mechanism for simultaneous patterning of auxin and cytokinin concentrations in Arabidopsis root development and suggests a key role for ethylene pattern integration. [Abstract copyright: Copyright © 2024. Published by Elsevier Inc.]

Citation

Moore, S., Jervis, G., Topping, J., Chen, C., Liu, J., & Lindsey, K. (2024). A predictive model for ethylene-mediated auxin and cytokinin patterning in the Arabidopsis root. Plant communications, 5(7), Article 100886. https://doi.org/10.1016/j.xplc.2024.100886

• Journal Article Type: Article
• Acceptance Date: Mar 18, 2024
• Online Publication Date: Mar 19, 2024
• Publication Date: Jul 8, 2024
• Deposit Date: Mar 20, 2024
• Publicly Available Date: Mar 20, 2024
• Journal: Plant communications
• Electronic ISSN: 2590-3462
• Publisher: Cell Press
• Peer Reviewed: Peer Reviewed
• Volume: 5
• Issue: 7
• Article Number: 100886
• DOI: https://doi.org/10.1016/j.xplc.2024.100886
• Keywords: spatiotemporal modelling, Arabidopsis root, ethylene signalling, in silico digital root, auxin patterning, cytokinin patterning
• Public URL: https://durham-repository.worktribe.com/output/2333223