The elicitor VP2 from Verticillium dahliae triggers defence response in cotton

Qiu, Ping; Zheng, Baoxin; Yuan, Hang; Yang, Zhaoguang; Lindsey, Keith; Wang, Yan; Ming, Yuqing; Zhang, Lin; Hu, Qin; Shaban, Muhammad; Kong, Jie; Zhang, Xianlong; Zhu, Longfu

Abstract

Summary: Verticillium dahliae is a widespread and destructive soilborne vascular pathogenic fungus that causes serious diseases in dicot plants. Here, comparative transcriptome analysis showed that the number of genes upregulated in defoliating pathotype V991 was significantly higher than in the non‐defoliating pathotype 1cd3‐2 during the early response of cotton. Combined with analysis of the secretome during the V991–cotton interaction, an elicitor VP2 was identified, which was highly upregulated at the early stage of V991 invasion, but was barely expressed during the 1cd3‐2‐cotton interaction. Full‐length VP2 could induce cell death in several plant species, and which was dependent on NbBAK1 but not on NbSOBIR1 in N. benthamiana. Knock‐out of VP2 attenuated the pathogenicity of V991. Furthermore, overexpression of VP2 in cotton enhanced resistance to V. dahliae without causing abnormal plant growth and development. Several genes involved in JA, SA and lignin synthesis were significantly upregulated in VP2‐overexpressing cotton. The contents of JA, SA, and lignin were also significantly higher than in the wild‐type control. In summary, the identified elicitor VP2, recognized by the receptor in the plant membrane, triggers the cotton immune response and enhances disease resistance.

Citation

Qiu, P., Zheng, B., Yuan, H., Yang, Z., Lindsey, K., Wang, Y., Ming, Y., Zhang, L., Hu, Q., Shaban, M., Kong, J., Zhang, X., & Zhu, L. (2024). The elicitor VP2 from Verticillium dahliae triggers defence response in cotton. Plant Biotechnology Journal, 22(2), 497-511. https://doi.org/10.1111/pbi.14201

• Journal Article Type: Article
• Acceptance Date: Sep 29, 2023
• Online Publication Date: Oct 26, 2023
• Publication Date: 2024-02
• Deposit Date: Oct 30, 2023
• Publicly Available Date: Oct 30, 2023
• Journal: Plant Biotechnology Journal
• Print ISSN: 1467-7644
• Electronic ISSN: 1467-7652
• Publisher: Association of Applied Biologists
• Peer Reviewed: Peer Reviewed
• Volume: 22
• Issue: 2
• Pages: 497-511
• DOI: https://doi.org/10.1111/pbi.14201
• Keywords: cotton, induced resistance, elicitor, comparative transcriptome analysis, germplasm innovation, Verticillium dahliae
• Public URL: https://durham-repository.worktribe.com/output/1863205