Single-cell RNA-seq reveals fate determination control of an individual fiber cell initiation in cotton (Gossypium hirsutum)

Qin, Y.; Sun, M.; Li, W.; Xu, M.; Shao, L.; Liu, Y.; Zhao, G.; Liu, Z.; Xu, Z.; You, J.; Ye, Z.; Xu, J.; Yang, X.; Wang, M.; Lindsey, K.; Zhang, X.; Tu, L.

Abstract

Cotton fiber is a unicellular seed trichome, and lint fiber initials per seed as a factor determines fiber yield. However, the mechanisms controlling fiber initiation from ovule epidermis are not understood well enough. Here, with single-cell RNA sequencing (scRNA-seq), a total of 14,535 cells were identified from cotton ovule outer integument of Xu142_LF line at four developmental stages (1.5, 1, 0.5 days before anthesis and the day of anthesis). Three major cell types, fiber, non-fiber epidermis and outer pigment layer were identified and then verified by RNA in situ hybridization. A comparative analysis on scRNA-seq data between Xu142 and its fiberless mutant Xu142 fl further confirmed fiber cluster definition. The developmental trajectory of fiber cell was reconstructed, and fiber cell was identified differentiated at 1 day before anthesis. Gene regulatory networks at four stages revealed the spatiotemporal pattern of core transcription factors, and MYB25-like and HOX3 were demonstrated played key roles as commanders in fiber differentiation and tip-biased diffuse growth, respectively. A model for early development of a single fiber cell was proposed here, which sheds light on further deciphering mechanism of plant trichome and the improvement of cotton fiber yield.

Citation

Qin, Y., Sun, M., Li, W., Xu, M., Shao, L., Liu, Y., Zhao, G., Liu, Z., Xu, Z., You, J., Ye, Z., Xu, J., Yang, X., Wang, M., Lindsey, K., Zhang, X., & Tu, L. (2022). Single-cell RNA-seq reveals fate determination control of an individual fiber cell initiation in cotton (Gossypium hirsutum). Plant Biotechnology Journal, 20(12), 2372-2388. https://doi.org/10.1111/pbi.13918

• Journal Article Type: Article
• Acceptance Date: Aug 22, 2022
• Online Publication Date: Aug 29, 2022
• Publication Date: 2022-12
• Deposit Date: Aug 23, 2022
• Publicly Available Date: Sep 20, 2022
• Journal: Plant Biotechnology Journal
• Print ISSN: 1467-7644
• Electronic ISSN: 1467-7652
• Publisher: Association of Applied Biologists
• Peer Reviewed: Peer Reviewed
• Volume: 20
• Issue: 12
• Pages: 2372-2388
• DOI: https://doi.org/10.1111/pbi.13918
• Public URL: https://durham-repository.worktribe.com/output/1193749