Dr Tim Hawkins' Outputs (28)

New imaging tools reveal live cellular collagen secretion, fibril dynamics and network organisation (2024)
Preprint / Working Paper
Kent, O., Casey, E. R., Brown, M., Bell, S., Ehrman, M., Flagler, M., Määttä, A., Benham, A. M., & Hawkins, T. J. New imaging tools reveal live cellular collagen secretion, fibril dynamics and network organisation

Although light microscopy has been used to examine the early trafficking of collagen within the cell, much of our understanding of the detailed organisation of cell deposited collagen is from static electron microscopy studies. To understand the dyna... Read More about New imaging tools reveal live cellular collagen secretion, fibril dynamics and network organisation.

Para-Hydroxycinnamic Acid Mitigates Senescence and Inflammaging in Human Skin Models (2024)
Journal Article
Tan, C. Y. R., Morenc, M., Setiawan, M., Lim, Z. Z. Y., Soon, A. L., Bierman, J. C., Vires, L., Laughlin, T., DeAngelis, Y. M., Rovito, H., Jarrold, B. B., Nguyen, T. Q. N., Lim, J. S. Y., Kent, O., Määttä, A., Benham, A. M., Hawkins, T. J., Lee, X. E., Ehrman, M. C., Oblong, J. E., …Bellanger, S. (2024). Para-Hydroxycinnamic Acid Mitigates Senescence and Inflammaging in Human Skin Models. International Journal of Molecular Sciences, 25(15), Article 8153. https://doi.org/10.3390/ijms25158153

Para-hydroxycinnamic acid (pHCA) is one of the most abundant naturally occurring hydroxycinnamic acids, a class of chemistries known for their antioxidant properties. In this study, we evaluated the impact of pHCA on different parameters of skin agin... Read More about Para-Hydroxycinnamic Acid Mitigates Senescence and Inflammaging in Human Skin Models.

NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity (2023)
Journal Article
Hawkins, T. J., Kopischke, M., Duckney, P. J., Rybak, K., Mentlak, D. A., Kroon, J. T. M., …Robatzek, S. (2023). NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity. Nature Communications, 14(1), Article 5848. https://doi.org/10.1038/s41467-023-41337-z

Members of the NETWORKED (NET) family are involved in actin-membrane interactions. Here we show that two members of the NET family, NET4A and NET4B, are essential for normal guard cell actin reorganization, which is a process critical for stomatal cl... Read More about NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity.

Expansion Microscopy of Plant Cells (PlantExM) (2023)
Book Chapter
Hawkins, T. J., Robson, J. L., Cole, B., & Bush, S. J. (2023). Expansion Microscopy of Plant Cells (PlantExM). In The Plant Cytoskeleton (127-142). Springer. https://doi.org/10.1007/978-1-0716-2867-6_10

Expansion microscopy (ExM) achieves super-resolution imaging without the need for sophisticated super-resolution microscopy hardware through a combination of physical and optical magnification. Samples are fixed, stained, and embedded in a swellable... Read More about Expansion Microscopy of Plant Cells (PlantExM).

A Potential Role of Keratinocyte-Derived Bilirubin in Human Skin Yellowness and Its Amelioration by Sucrose Laurate/Dilaurate (2022)
Journal Article
Fang, B., Card, P. D., Chen, J., Li, L., Laughlin, T., Jarrold, B., Zhao, W., Benham, A. M., Määttä, A. T., Hawkins, T. J., & Hakozaki, T. (2022). A Potential Role of Keratinocyte-Derived Bilirubin in Human Skin Yellowness and Its Amelioration by Sucrose Laurate/Dilaurate. International Journal of Molecular Sciences, 23(11), Article 5884. https://doi.org/10.3390/ijms23115884

Sallow and/or dull skin appearance is greatly attributable to the yellow components of skin tone. Bilirubin is a yellow chromophore known to be made in the liver and/or spleen and is transported throughout the body via the blood stream. Recent public... Read More about A Potential Role of Keratinocyte-Derived Bilirubin in Human Skin Yellowness and Its Amelioration by Sucrose Laurate/Dilaurate.

NETWORKED2‐Subfamily Proteins Regulate the Cortical Actin Cytoskeleton of Growing Pollen Tubes and Polarised Pollen Tube Growth (2021)
Journal Article
Duckney, P., Kroon, J. T., Dixon, M. R., Hawkins, T. J., Deeks, M. J., & Hussey, P. J. (2021). NETWORKED2‐Subfamily Proteins Regulate the Cortical Actin Cytoskeleton of Growing Pollen Tubes and Polarised Pollen Tube Growth. New Phytologist, 231(1), 152-164. https://doi.org/10.1111/nph.17391

We have recently characterised NET2A as a pollen‐specific actin‐binding protein which binds F‐actin at the plasma membrane of growing pollen tubes. However, the role of NET2 proteins in pollen development and fertilisation have yet to be elucidated.... Read More about NETWORKED2‐Subfamily Proteins Regulate the Cortical Actin Cytoskeleton of Growing Pollen Tubes and Polarised Pollen Tube Growth.

Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata (2019)
Journal Article
Brault, M. L., Petit, J. D., Immel, F., Nicolas, W. J., Glavier, M., Brocard, L., Gaston, A., Fouché, M., Hawkins, T. J., Crowet, J., Grison, M. S., Germain, V., Rocher, M., Kraner, M., Alva, V., Claverol, S., Paterlini, A., Helariutta, Y., Deleu, M., Lins, L., …Bayer, E. M. (2019). Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata. EMBO Reports, 20(8), Article e47182. https://doi.org/10.15252/embr.201847182

In eukaryotes, membrane contact sites (MCS) allow direct communication between organelles. Plants have evolved a unique type of MCS, inside intercellular pores, the plasmodesmata, where endoplasmic reticulum (ER)–plasma membrane (PM) contacts coincid... Read More about Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata.

Polar cell growth and the cytoskeleton (2019)
Book Chapter
Hussey, P., Deeks, M., Hawkins, T., & Ketelaar, T. (2019). Polar cell growth and the cytoskeleton. In K. Lindsey (Ed.), Polarity in Plants. Blackwell

Pulling force deforms hair follicle root sheath nuclei and surrounding dermal collagen matrix differently at infundibulum, isthmus and suprabulbar regions (2019)
Journal Article
Johnson, S., Cowley, K., Hawkins, T. J., & Määttä, A. (2019). Pulling force deforms hair follicle root sheath nuclei and surrounding dermal collagen matrix differently at infundibulum, isthmus and suprabulbar regions. Experimental Dermatology, 28(7), 862-866. https://doi.org/10.1111/exd.13948

The biomechanical properties of the collagenous dermal matrix are well described but responses to mechanical force by the hair follicles have not been characterised so far. We applied a pulling force on hair follicles to visualise and quantify change... Read More about Pulling force deforms hair follicle root sheath nuclei and surrounding dermal collagen matrix differently at infundibulum, isthmus and suprabulbar regions.

Leaves of isoprene‐emitting tobacco plants maintain PSII stability at high temperatures. (2019)
Journal Article
Pollastri, S., Jorba, I., Hawkins, T., Llusia, J., Michelozzi, M., Navajas, D., Penuelas, J., Hussey, P., Knight, M., & Loreto, F. (2019). Leaves of isoprene‐emitting tobacco plants maintain PSII stability at high temperatures. New Phytologist, 223(3), 1307-1318. https://doi.org/10.1111/nph.15847

Depilatory chemical thioglycolate affects hair cuticle and cortex, degrades epidermal cornified envelopes and induces proliferation and differentiation responses in keratinocytes (2018)
Journal Article
Duit, R., Hawkins, T. J., & Määttä, A. (2019). Depilatory chemical thioglycolate affects hair cuticle and cortex, degrades epidermal cornified envelopes and induces proliferation and differentiation responses in keratinocytes. Experimental Dermatology, 28(1), 76-79. https://doi.org/10.1111/exd.13838

Thioglycolate is a potent depilatory agent. In addition, it has been proposed to be useful as a penetration enhancer for transepidermal drug delivery. However, the effects on hair structure and stress responses it elicits in epidermal keratinocytes h... Read More about Depilatory chemical thioglycolate affects hair cuticle and cortex, degrades epidermal cornified envelopes and induces proliferation and differentiation responses in keratinocytes.

Agitation Modules: Flexible Means to Accelerate Automated Freeze Substitution (2018)
Journal Article
Reipert, S., Goldammer, H., Richardson, C., Goldberg, M. W., Hawkins, T. J., Hollergschwandtner, E., Kaufmann, W. A., Antreich, S., & Stierhof, Y.-D. (2018). Agitation Modules: Flexible Means to Accelerate Automated Freeze Substitution. Journal of Histochemistry & Cytochemistry, 66(12), 903-921. https://doi.org/10.1369/0022155418786698

For ultrafast fixation of biological samples to avoid artifacts, high-pressure freezing (HPF) followed by freeze substitution (FS) is preferred over chemical fixation at room temperature. After HPF, samples are maintained at low temperature during de... Read More about Agitation Modules: Flexible Means to Accelerate Automated Freeze Substitution.

Recessive Mutation in FAM83G Associated with Palmoplantar Keratoderma and Exuberant Scalp Hair (2018)
Journal Article
Maruthappu, T., McGinty, L. A., Blaydon, D. C., Fell, B., Määttä, A., Duit, R., …Kelsell, D. P. (2018). Recessive Mutation in FAM83G Associated with Palmoplantar Keratoderma and Exuberant Scalp Hair. Journal of Investigative Dermatology, 138(4), 984-987. https://doi.org/10.1016/j.jid.2017.10.031

To the Editor Palmoplantar keratodermas are a heterogeneous group of disorders characterized by abnormal thickening of the volar epidermis (Blaydon and Kelsell, 2014, Maruthappu et al., 2014). A subset of palmoplantar keratodermas are associated with... Read More about Recessive Mutation in FAM83G Associated with Palmoplantar Keratoderma and Exuberant Scalp Hair.

A rim-and-spoke hypothesis to explain the biomechanical roles for cytoplasmic intermediate filament networks (2017)
Journal Article
Quinlan, R., Schwartz, N., Windoffer, R., Richardson, C., Hawkins, T., Broussard, J., …Leube, R. (2017). A rim-and-spoke hypothesis to explain the biomechanical roles for cytoplasmic intermediate filament networks. Journal of Cell Science, 130(20), 3437-3445. https://doi.org/10.1242/jcs.202168

Textbook images of keratin intermediate filament (IF) networks in epithelial cells and the functional compromization of the epidermis by keratin mutations promulgate a mechanical role for this important cytoskeletal component. In stratified epithelia... Read More about A rim-and-spoke hypothesis to explain the biomechanical roles for cytoplasmic intermediate filament networks.

Actin–membrane interactions mediated by NETWORKED2 in Arabidopsis pollen tubes through associations with Pollen Receptor-Like Kinase 4 and 5 (2017)
Journal Article
Duckney, P., Deeks, M., Dixon, M., Kroon, J., Hawkins, T., & Hussey, P. (2017). Actin–membrane interactions mediated by NETWORKED2 in Arabidopsis pollen tubes through associations with Pollen Receptor-Like Kinase 4 and 5. New Phytologist, 216(4), 1170-1180. https://doi.org/10.1111/nph.14745

During fertilization, Pollen Receptor-Like Kinases (PRKs) control pollen tube growth through the pistil in response to extracellular signals, and regulate the actin cytoskeleton at the tube apex to drive tip growth. We investigated a novel link betwe... Read More about Actin–membrane interactions mediated by NETWORKED2 in Arabidopsis pollen tubes through associations with Pollen Receptor-Like Kinase 4 and 5.

Connecting membranes to the actin cytoskeleton. (2017)
Journal Article
Wang, P., Hawkins, T., & Hussey, P. (2017). Connecting membranes to the actin cytoskeleton. Current Opinion in Plant Biology, 40, 71-76. https://doi.org/10.1016/j.pbi.2017.07.008

In plants, the actin cytoskeleton plays a major role in organelle movement, cargo transport, maintaining cell polarity and controlling the morphogenesis of endomembrane systems. All of these events require a direct connection between membrane structu... Read More about Connecting membranes to the actin cytoskeleton..

Microcompartmentation of cytosolic aldolase by interaction with the actin cytoskeleton in Arabidopsis (2017)
Journal Article
Garagounis, C., Kostaki, K., Hawkins, T. J., Cummins, I., Fricker, M. D., Hussey, P. J., …Sweetlove, L. J. (2017). Microcompartmentation of cytosolic aldolase by interaction with the actin cytoskeleton in Arabidopsis. Journal of Experimental Botany, 68(5), 885-898. https://doi.org/10.1093/jxb/erx015

Evidence is accumulating for molecular microcompartments formed when proteins interact in localized domains with the cytoskeleton, organelle surfaces, and intracellular membranes. To understand the potential functional significance of protein microco... Read More about Microcompartmentation of cytosolic aldolase by interaction with the actin cytoskeleton in Arabidopsis.

Plant VAP27 proteins: domain characterization, intracellular localization and role in plant development (2016)
Journal Article
Wang, P., Richardson, C., Hawkins, T. J., Sparkes, I., Hawes, C., & Hussey, P. J. (2016). Plant VAP27 proteins: domain characterization, intracellular localization and role in plant development. New Phytologist, 210(4), 1311-1326. https://doi.org/10.1111/nph.13857

The endoplasmic reticulum (ER) is connected to the plasma membrane (PM) through the plant-specific NETWORKED protein, NET3C, and phylogenetically conserved vesicle-associated membrane protein-associated proteins (VAPs). Ten VAP homologues (VAP27-1 to... Read More about Plant VAP27 proteins: domain characterization, intracellular localization and role in plant development.

Blobs and curves: object-based colocalisation for plant cells (2014)
Journal Article
Nelson, C., Duckney, P., Hawkins, T., Deeks, M., Laissue, P., Hussey, P., & Obara, B. (2014). Blobs and curves: object-based colocalisation for plant cells. Functional Plant Biology, 42(5), 471-485. https://doi.org/10.1071/fp14047

Blobs and curves occur everywhere in plant bioimaging: from signals of fluorescence-labelled proteins, through cytoskeletal structures, nuclei staining and cell extensions such as root hairs. Here we look at the problem of colocalisation of blobs wit... Read More about Blobs and curves: object-based colocalisation for plant cells.

The Plant Cytoskeleton, NET3C, and VAP27 Mediate the Link between the Plasma Membrane and Endoplasmic Reticulum (2014)
Journal Article
Wang, P., Hawkins, T. J., Richardson, C., Cummins, I., Deeks, M. J., Sparkes, I., …Hussey, P. J. (2014). The Plant Cytoskeleton, NET3C, and VAP27 Mediate the Link between the Plasma Membrane and Endoplasmic Reticulum. Current Biology, 24(12), 1397-1405. https://doi.org/10.1016/j.cub.2014.05.003

The cortical endoplasmic reticulum (ER) network in plants is a highly dynamic structure, and it contacts the plasma membrane (PM) at ER-PM anchor/contact sites. These sites are known to be essential for communication between the ER and PM for lipid t... Read More about The Plant Cytoskeleton, NET3C, and VAP27 Mediate the Link between the Plasma Membrane and Endoplasmic Reticulum.