Blobs and curves: object-based colocalisation for plant cells

Nelson, CJ; Duckney, P; Hawkins, TJ; Deeks, MJ; Laissue, PP; Hussey, PJ; Obara, B

Abstract

Blobs and curves occur everywhere in plant bioimaging: from signals of fluorescence-labelled proteins, through cytoskeletal structures, nuclei staining and cell extensions such as root hairs. Here we look at the problem of colocalisation of blobs with blobs (protein-protein colocalisation) and blobs with curves (organelle-cytoskeleton colocalisation). This article demonstrates a clear quantitative alternative to pixel-based colocalisation methods and, using object-based methods, can quantify not only the level of colocalisation but also the distance between objects. Included in this report are computational algorithms, biological experiments and guidance for those looking to increase their use of computationally-based and quantified analysis of bioimages.

Citation

Nelson, C., Duckney, P., Hawkins, T., Deeks, M., Laissue, P., Hussey, P., & Obara, B. (2014). Blobs and curves: object-based colocalisation for plant cells. Functional Plant Biology, 42(5), 471-485. https://doi.org/10.1071/fp14047

• Journal Article Type: Article
• Acceptance Date: Jul 21, 2014
• Online Publication Date: Sep 22, 2014
• Publication Date: Sep 22, 2014
• Deposit Date: Jan 28, 2015
• Publicly Available Date: Mar 23, 2018
• Journal: Functional Plant Biology
• Print ISSN: 1445-4408
• Electronic ISSN: 1445-4416
• Publisher: CSIRO Publishing
• Peer Reviewed: Peer Reviewed
• Volume: 42
• Issue: 5
• Pages: 471-485
• DOI: https://doi.org/10.1071/fp14047
• Public URL: https://durham-repository.worktribe.com/output/1413558