Marie L Brault
Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata
Brault, Marie L; Petit, Jules D; Immel, Françoise; Nicolas, William J; Glavier, Marie; Brocard, Lysiane; Gaston, Amèlia; Fouché, Mathieu; Hawkins, Timothy J; Crowet, Jean‐Marc; Grison, Magali S; Germain, Véronique; Rocher, Marion; Kraner, Max; Alva, Vikram; Claverol, Stéphane; Paterlini, Andrea; Helariutta, Ykä; Deleu, Magali; Lins, Laurence; Tilsner, Jens; Bayer, Emmanuelle M
Authors
Jules D Petit
Françoise Immel
William J Nicolas
Marie Glavier
Lysiane Brocard
Amèlia Gaston
Mathieu Fouché
Dr Tim Hawkins t.j.hawkins@durham.ac.uk
Head of Bioimaging
Jean‐Marc Crowet
Magali S Grison
Véronique Germain
Marion Rocher
Max Kraner
Vikram Alva
Stéphane Claverol
Andrea Paterlini
Ykä Helariutta
Magali Deleu
Laurence Lins
Jens Tilsner
Emmanuelle M Bayer
Abstract
In eukaryotes, membrane contact sites (MCS) allow direct communication between organelles. Plants have evolved a unique type of MCS, inside intercellular pores, the plasmodesmata, where endoplasmic reticulum (ER)–plasma membrane (PM) contacts coincide with regulation of cell‐to‐cell signalling. The molecular mechanism and function of membrane tethering within plasmodesmata remain unknown. Here, we show that the multiple C2 domains and transmembrane region protein (MCTP) family, key regulators of cell‐to‐cell signalling in plants, act as ER‐PM tethers specifically at plasmodesmata. We report that MCTPs are plasmodesmata proteins that insert into the ER via their transmembrane region while their C2 domains dock to the PM through interaction with anionic phospholipids. A Atmctp3/Atmctp4 loss of function mutant induces plant developmental defects, impaired plasmodesmata function and composition, while MCTP4 expression in a yeast Δtether mutant partially restores ER‐PM tethering. Our data suggest that MCTPs are unique membrane tethers controlling both ER‐PM contacts and cell‐to‐cell signalling.
Citation
Brault, M. L., Petit, J. D., Immel, F., Nicolas, W. J., Glavier, M., Brocard, L., …Bayer, E. M. (2019). Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata. EMBO Reports, 20(8), Article e47182. https://doi.org/10.15252/embr.201847182
Journal Article Type | Article |
---|---|
Acceptance Date | Jun 6, 2019 |
Online Publication Date | Jul 9, 2019 |
Publication Date | Aug 1, 2019 |
Deposit Date | Jul 11, 2019 |
Publicly Available Date | Jul 24, 2019 |
Journal | EMBO Reports |
Print ISSN | 1469-221X |
Electronic ISSN | 1469-3178 |
Publisher | Wiley |
Peer Reviewed | Peer Reviewed |
Volume | 20 |
Issue | 8 |
Article Number | e47182 |
DOI | https://doi.org/10.15252/embr.201847182 |
Public URL | https://durham-repository.worktribe.com/output/1292592 |
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Copyright Statement
Advance online version © 2019 The Authors. Published under the terms of the CC BY 4.0 license.
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