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Outputs (137)

Suppressed basal mitophagy drives cellular aging phenotypes that can be reversed by a p62-targeting small molecule. (2024)
Journal Article
Kelly, G., Kataura, T., Panek, J., Ma, G., Salmonowicz, H., Davis, A., …Korolchuk, V. I. (2024). Suppressed basal mitophagy drives cellular aging phenotypes that can be reversed by a p62-targeting small molecule. Developmental Cell, Article S1534-5807(24)00295-8. https://doi.org/10.1016/j.devcel.2024.04.020

Selective degradation of damaged mitochondria by autophagy (mitophagy) is proposed to play an important role in cellular homeostasis. However, the molecular mechanisms and the requirement of mitochondrial quality control by mitophagy for cellular phy... Read More about Suppressed basal mitophagy drives cellular aging phenotypes that can be reversed by a p62-targeting small molecule..

Current understanding of the interplay between extracellular matrix remodelling and gut permeability in health and disease (2024)
Journal Article
Vilardi, A., Przyborski, S., Mobbs, C., Rufini, A., & Tufarelli, C. (2024). Current understanding of the interplay between extracellular matrix remodelling and gut permeability in health and disease. Cell Death Discovery, 10(1), Article 258. https://doi.org/10.1038/s41420-024-02015-1

The intestinal wall represents an interactive network regulated by the intestinal epithelium, extracellular matrix (ECM) and mesenchymal compartment. Under healthy physiological conditions, the epithelium undergoes constant renewal and forms an integ... Read More about Current understanding of the interplay between extracellular matrix remodelling and gut permeability in health and disease.

Comparison of photodamage in non-pigmented and pigmented human skin equivalents exposed to repeated ultraviolet radiation to investigate the role of melanocytes in skin photoprotection (2024)
Journal Article
De Los Santos Gomez, P., Costello, L., Goncalves, K., & Przyborski, S. (2024). Comparison of photodamage in non-pigmented and pigmented human skin equivalents exposed to repeated ultraviolet radiation to investigate the role of melanocytes in skin photoprotection. Frontiers in Medicine, 11, Article 1355799. https://doi.org/10.3389/fmed.2024.1355799

Introduction: Daily solar ultraviolet (UV) radiation has an important impact on skin health. Understanding the initial events of the UV-induced response is critical to prevent deleterious conditions. However, studies in human volunteers have ethical,... Read More about Comparison of photodamage in non-pigmented and pigmented human skin equivalents exposed to repeated ultraviolet radiation to investigate the role of melanocytes in skin photoprotection.

Cell Senescence-Independent Changes of Human Skin Fibroblasts with Age (2024)
Journal Article
Fullard, N., Wordsworth, J., Welsh, C., Maltman, V., Bascom, C., Tasseff, R., …Shanley, D. (2024). Cell Senescence-Independent Changes of Human Skin Fibroblasts with Age. Cells, 13(8), Article 659. https://doi.org/10.3390/cells13080659

Skin ageing is defined, in part, by collagen depletion and fragmentation that leads to a loss of mechanical tension. This is currently believed to reflect, in part, the accumulation of senescent cells. We compared the expression of genes and proteins... Read More about Cell Senescence-Independent Changes of Human Skin Fibroblasts with Age.

Bioprinted autologous human skin equivalents for in vitro testing of therapeutic antibodies (2024)
Journal Article
Ahmed, M., Hill, D., Ahmed, S., Przyborski, S., Dalgarno, K., & Dickinson, A. (2024). Bioprinted autologous human skin equivalents for in vitro testing of therapeutic antibodies. International Journal of Bioprinting, 10(2), Article 1851. https://doi.org/10.36922/ijb.1851

In recent years, advances in tissue engineering have brought forward the accessibility of human skin equivalents for in vitro applications; however, the availability of human-based engineered tissue models suitable for high-throughput screening of bi... Read More about Bioprinted autologous human skin equivalents for in vitro testing of therapeutic antibodies.

An in vitro model to study immune activation, epithelial disruption and stromal remodelling in inflammatory bowel disease and fistulising Crohn’s disease (2024)
Journal Article
Mobbs, C. L., Darling, N. J., & Przyborski, S. (2024). An in vitro model to study immune activation, epithelial disruption and stromal remodelling in inflammatory bowel disease and fistulising Crohn’s disease. Frontiers in Immunology, 15, Article 1357690. https://doi.org/10.3389/fimmu.2024.1357690

At present, preclinical models of inflammatory bowel disease (IBD) are insufficient, limiting translation between research and new therapeutics. This is especially true for fistulising Crohn’s disease (CD), as the severe lack of relevant models hinde... Read More about An in vitro model to study immune activation, epithelial disruption and stromal remodelling in inflammatory bowel disease and fistulising Crohn’s disease.

Development of a novel in vitro strategy to understand the impact of shaving on skin health: combining tape strip exfoliation and human skin equivalent technology (2023)
Journal Article
Costello, L., Goncalves, K., Maltman, V., Barrett, N., Shah, K., Stephens, A., …Przyborski, S. (2023). Development of a novel in vitro strategy to understand the impact of shaving on skin health: combining tape strip exfoliation and human skin equivalent technology. Frontiers in Medicine, 10, Article 1236790. https://doi.org/10.3389/fmed.2023.1236790

Introduction: The removal of unwanted hair is a widespread grooming practice adopted by both males and females. Although many depilatory techniques are now available, shaving remains the most common, despite its propensity to irritate skin. Current t... Read More about Development of a novel in vitro strategy to understand the impact of shaving on skin health: combining tape strip exfoliation and human skin equivalent technology.

Impact of the Physical Cellular Microenvironment on the Structure and Function of a Model Hepatocyte Cell Line for Drug Toxicity Applications (2023)
Journal Article
Allcock, B., Wei, W., Goncalves, K., Hoyle, H., Robert, A., Quelch-Cliffe, R., …Przyborski, S. (2023). Impact of the Physical Cellular Microenvironment on the Structure and Function of a Model Hepatocyte Cell Line for Drug Toxicity Applications. Cells, 12(19), Article 2408. https://doi.org/10.3390/cells12192408

It is widely recognised that cells respond to their microenvironment, which has implications for cell culture practices. Growth cues provided by 2D cell culture substrates are far removed from native 3D tissue structure in vivo. Geometry is one of ma... Read More about Impact of the Physical Cellular Microenvironment on the Structure and Function of a Model Hepatocyte Cell Line for Drug Toxicity Applications.

Quantitative morphometric analysis of intrinsic and extrinsic skin ageing in individuals with Fitzpatrick skin types II–III (2023)
Journal Article
Costello, L., Goncalves, K., De Los Santos Gomez, P., Simpson, A., Maltman, V., Ritchie, P., …Przyborski, S. (2023). Quantitative morphometric analysis of intrinsic and extrinsic skin ageing in individuals with Fitzpatrick skin types II–III. Experimental Dermatology, 32(5), 620-631. https://doi.org/10.1111/exd.14754

Skin ageing is an intricate physiological process affected by intrinsic and extrinsic factors. There is a demand to understand how the skin changes with age and photoexposure in individuals with Fitzpatrick skin types I-III due to accelerated photoag... Read More about Quantitative morphometric analysis of intrinsic and extrinsic skin ageing in individuals with Fitzpatrick skin types II–III.

Regulation of epidermal proliferation and hair follicle cycling by synthetic photostable retinoid EC23 (2023)
Journal Article
Määttä, A., Nixon, R., Robinson, N., Ambler, C. A., Goncalves, K., Maltman, V., & Przyborski, S. (2023). Regulation of epidermal proliferation and hair follicle cycling by synthetic photostable retinoid EC23. Journal of Cosmetic Dermatology, 22(5), https://doi.org/10.1111/jocd.15629

Background Retinoid signaling is an important regulator of the epidermis and skin appendages. Therefore, synthetic retinoids have been developed for therapeutic use for skin disorders such as psoriasis and acne. Aims In previous studies, we showed ho... Read More about Regulation of epidermal proliferation and hair follicle cycling by synthetic photostable retinoid EC23.

Application of biomimetic surfaces and 3D culture technology to study the role of extracellular matrix interactions in neurite outgrowth and inhibition (2022)
Journal Article
Goncalves, K., Phillips, S., Shah, D., Athey, D., & Przyborski, S. (2023). Application of biomimetic surfaces and 3D culture technology to study the role of extracellular matrix interactions in neurite outgrowth and inhibition. Biomaterials Advances, 144, Article 213204. https://doi.org/10.1016/j.bioadv.2022.213204

The microenvironment that cells experience during in vitro culture can often be far removed from the native environment they are exposed to in vivo. To recreate the physiological environment that developing neurites experience in vivo, we combine a w... Read More about Application of biomimetic surfaces and 3D culture technology to study the role of extracellular matrix interactions in neurite outgrowth and inhibition.

Investigation into the effect of skin tone modulators and exogenous stress on skin pigmentation utilizing a novel bioengineered skin equivalent (2022)
Journal Article
Goncalves, K., De Los Santos Gomez, P., Costello, L., Smith, L., Mead, H., Simpson, A., & Przyborski, S. (2023). Investigation into the effect of skin tone modulators and exogenous stress on skin pigmentation utilizing a novel bioengineered skin equivalent. Bioengineering and Translational Medicine, 8(2), Article e10415. https://doi.org/10.1002/btm2.10415

Human skin equivalents (HSEs) are a popular technology due to limitations in animal testing, particularly as they recapitulate aspects of structure and function of human skin. Many HSEs contain two basic cell types to model dermal and epidermal compa... Read More about Investigation into the effect of skin tone modulators and exogenous stress on skin pigmentation utilizing a novel bioengineered skin equivalent.

Design considerations of benchtop fluid flow bioreactors for bio-engineered tissue equivalents in vitro (2022)
Journal Article
Hoyle, H., Stenger, C., & Przyborski, S. (2022). Design considerations of benchtop fluid flow bioreactors for bio-engineered tissue equivalents in vitro. Biomaterials and biosystems, 8, Article 100063. https://doi.org/10.1016/j.bbiosy.2022.100063

One of the major aims of bio-engineering tissue equivalents in vitro is to create physiologically relevant culture conditions to accurately recreate the cellular microenvironment. This often includes incorporation of factors such as the extracellular... Read More about Design considerations of benchtop fluid flow bioreactors for bio-engineered tissue equivalents in vitro.

Early onset of senescence and imbalanced epidermal homeostasis across the decades in photoexposed human skin: Fingerprints of inflammaging (2022)
Journal Article
Jarrold, B. B., Tan, C. Y. R., Ho, C. Y., Soon, A. L., Lam, T. T., Yang, X., …Oblong, J. E. (2022). Early onset of senescence and imbalanced epidermal homeostasis across the decades in photoexposed human skin: Fingerprints of inflammaging. Experimental Dermatology, 31(11), 1748-1760. https://doi.org/10.1111/exd.14654

Inflammaging is a theory of ageing which purports that low-level chronic inflammation leads to cellular dysfunction and premature ageing of surrounding tissue. Skin is susceptible to inflammaging because it is the first line of defence from the envir... Read More about Early onset of senescence and imbalanced epidermal homeostasis across the decades in photoexposed human skin: Fingerprints of inflammaging.

Recreating Tissue Structures Representative of Teratomas In Vitro Using a Combination of 3D Cell Culture Technology and Human Embryonic Stem Cells (2022)
Journal Article
Hidalgo Aguilar, A., Smith, L., Owens, D., Quelch, R., & Przyborski, S. (2022). Recreating Tissue Structures Representative of Teratomas In Vitro Using a Combination of 3D Cell Culture Technology and Human Embryonic Stem Cells. Bioengineering, 9(5), Article 185. https://doi.org/10.3390/bioengineering9050185

In vitro studies using human embryonic stem cells (hESCs) are a valuable method to study aspects of embryogenesis, avoiding ethical issues when using embryonic materials and species dissimilarities. The xenograft teratoma assay is often traditionally... Read More about Recreating Tissue Structures Representative of Teratomas In Vitro Using a Combination of 3D Cell Culture Technology and Human Embryonic Stem Cells.

Tissue engineering strategies to bioengineer the ageing skin phenotype in vitro (2022)
Journal Article
Costello, L., Dicolandrea, T., Tasseff, R., Isfort, R., Bascom, C., von Zglinicki, T., & Przyborski, S. (2022). Tissue engineering strategies to bioengineer the ageing skin phenotype in vitro. Aging Cell, 21(2), Article e13550. https://doi.org/10.1111/acel.13550

Human skin ageing is a complex and heterogeneous process, which is influenced by genetically determined intrinsic factors and accelerated by cumulative exposure to extrinsic stressors. In the current world ageing demographic, there is a requirement f... Read More about Tissue engineering strategies to bioengineer the ageing skin phenotype in vitro.

Development of a mammalian neurosensory full‐thickness skin equivalent and its application to screen sensitizing stimuli (2022)
Journal Article
Freer, M., Darling, N., Goncalves, K., Mills, K. J., & Przyborski, S. (2023). Development of a mammalian neurosensory full‐thickness skin equivalent and its application to screen sensitizing stimuli. Bioengineering and Translational Medicine, 8(3), Article e10484. https://doi.org/10.1002/btm2.10484

Human skin equivalents (HSEs) are an increasingly popular research tool due to limitations associated with animal testing for dermatological research. They recapitulate many aspects of skin structure and function, however, many only contain two basic... Read More about Development of a mammalian neurosensory full‐thickness skin equivalent and its application to screen sensitizing stimuli.

How good is the evidence that cellular senescence causes skin ageing? (2021)
Journal Article
Low, E., Alimohammadiha, G., Smith, L. A., Costello, L. F., Przyborski, S. A., von Zglinicki, T., & Miwa, S. (2021). How good is the evidence that cellular senescence causes skin ageing?. Ageing Research Reviews, 71, https://doi.org/10.1016/j.arr.2021.101456

Skin is the largest organ of the body with important protective functions, which become compromised with time due to both intrinsic and extrinsic ageing processes. Cellular senescence is the primary ageing process at cell level, associated with loss... Read More about How good is the evidence that cellular senescence causes skin ageing?.

The Cell-Permeable Derivative of the Immunoregulatory Metabolite Itaconate, 4-Octyl Itaconate, Is Anti-Fibrotic in Systemic Sclerosis (2021)
Journal Article
Henderson, J., Dayalan Naidu, S., Dinkova-Kostova, A. T., Przyborski, S., Stratton, R., & O′Reilly, S. (2021). The Cell-Permeable Derivative of the Immunoregulatory Metabolite Itaconate, 4-Octyl Itaconate, Is Anti-Fibrotic in Systemic Sclerosis. Cells, 10(8), https://doi.org/10.3390/cells10082053

Systemic sclerosis (SSc) is an autoimmune connective tissue disease that leads to skin fibrosis. Altered metabolism has recently been described in autoimmune diseases and SSc. Itaconate is a product of the Krebs cycle intermediate cis-aconitate and i... Read More about The Cell-Permeable Derivative of the Immunoregulatory Metabolite Itaconate, 4-Octyl Itaconate, Is Anti-Fibrotic in Systemic Sclerosis.

Bone Morphogenetic Protein Antagonist Gremlin-1 Increases Myofibroblast Transition in Dermal Fibroblasts: Implications for Systemic Sclerosis (2021)
Journal Article
Duffy, L., Henderson, J., Brown, M., Pryzborski, S., Fullard, N., Summa, L., …O’Reilly, S. (2021). Bone Morphogenetic Protein Antagonist Gremlin-1 Increases Myofibroblast Transition in Dermal Fibroblasts: Implications for Systemic Sclerosis. Frontiers in Cell and Developmental Biology, 9, Article 681061. https://doi.org/10.3389/fcell.2021.681061

Objective: Systemic Sclerosis is an autoimmune connective tissue disease which results in fibrosis of the skin and lungs. The disease is characterized by activation of myofibroblasts but what governs this is unknown. Gremlin-1 is a BMP antagonist tha... Read More about Bone Morphogenetic Protein Antagonist Gremlin-1 Increases Myofibroblast Transition in Dermal Fibroblasts: Implications for Systemic Sclerosis.

Using Advanced Cell Culture Techniques to Differentiate Pluripotent Stem Cells and Recreate Tissue Structures Representative of Teratoma Xenografts (2021)
Journal Article
Smith, L., Hidalgo Aguilar, A., Owens, D., Quelch, R., Knight, E., & Przyborski, S. (2021). Using Advanced Cell Culture Techniques to Differentiate Pluripotent Stem Cells and Recreate Tissue Structures Representative of Teratoma Xenografts. Frontiers in Cell and Developmental Biology, 9, https://doi.org/10.3389/fcell.2021.667246

Various methods are currently used to investigate human tissue differentiation, including human embryo culture and studies utilising pluripotent stem cells (PSCs) such as in vitro embryoid body formation and in vivo teratoma assays. Each method has i... Read More about Using Advanced Cell Culture Techniques to Differentiate Pluripotent Stem Cells and Recreate Tissue Structures Representative of Teratoma Xenografts.

The vitamin A ester retinyl propionate has a unique metabolic profile and higher retinoid‐related bioactivity over retinol and retinyl palmitate in human skin models (2020)
Journal Article
Bjerke, D. L., Li, R., Price, J. M., Dobson, R. L., Rodrigues, M., Tey, C., …Oblong, J. E. (2021). The vitamin A ester retinyl propionate has a unique metabolic profile and higher retinoid‐related bioactivity over retinol and retinyl palmitate in human skin models. Experimental Dermatology, 30(2), 226-236. https://doi.org/10.1111/exd.14219

Human skin is exposed daily to environmental stressors, which cause acute damage and inflammation. Over time this leads to morphological and visual appearance changes associated with premature aging. Topical vitamin A derivatives such as retinol (ROL... Read More about The vitamin A ester retinyl propionate has a unique metabolic profile and higher retinoid‐related bioactivity over retinol and retinyl palmitate in human skin models.

microRNA27a-3p mediates reduction of the Wnt antagonist sFRP-1 in systemic sclerosis (2020)
Journal Article
Henderson, J., Wilkinson, S., Przyborski, S., Stratton, R., & O’Reilly, S. (2021). microRNA27a-3p mediates reduction of the Wnt antagonist sFRP-1 in systemic sclerosis. Epigenetics, 16(7), 808-817. https://doi.org/10.1080/15592294.2020.1827715

Systemic Sclerosis (SSc) is an autoimmune connective tissue disease that leads to skin and lung fibrosis. The Wnt pathway is clearly elevated in SSc and is pro-fibrotic via activation of canonical Wnt signalling. sFRP-1 is a Wnt antagonist that acts... Read More about microRNA27a-3p mediates reduction of the Wnt antagonist sFRP-1 in systemic sclerosis.

Bioengineering Novel in vitro Co-culture Models That Represent the Human Intestinal Mucosa With Improved Caco-2 Structure and Barrier Function (2020)
Journal Article
Darling, N. J., Mobbs, C. L., González-Hau, A. L., Freer, M., & Przyborski, S. (2020). Bioengineering Novel in vitro Co-culture Models That Represent the Human Intestinal Mucosa With Improved Caco-2 Structure and Barrier Function. Frontiers in Bioengineering and Biotechnology, 8, Article 992. https://doi.org/10.3389/fbioe.2020.00992

The Caco-2 monolayer is the most widely used in vitro model of the human intestinal mucosa to study absorption. However, models lack communication from other cells present in the native intestine, such as signals from fibroblasts in the lamina propri... Read More about Bioengineering Novel in vitro Co-culture Models That Represent the Human Intestinal Mucosa With Improved Caco-2 Structure and Barrier Function.

Germline TET2 loss of function causes childhood immunodeficiency and lymphoma (2020)
Journal Article
Stremenova Spegarova, J., Lawless, D., Mohamad, S. M. B., Engelhardt, K. R., Doody, G., Shrimpton, J., …Hambleton, S. (2020). Germline TET2 loss of function causes childhood immunodeficiency and lymphoma. Blood, 136(9), 1055-1066. https://doi.org/10.1182/blood.2020005844

Molecular dissection of inborn errors of immunity can help to elucidate the nonredundant functions of individual genes. We studied 3 children with an immune dysregulation syndrome of susceptibility to infection, lymphadenopathy, hepatosplenomegaly, d... Read More about Germline TET2 loss of function causes childhood immunodeficiency and lymphoma.

Multiple scattering in scanning helium microscopy (2020)
Journal Article
Lambrick, S., Vozdecký, L., Bergin, M., Halpin, J., MacLaren, D., Dastoor, P., …Ward, D. (2020). Multiple scattering in scanning helium microscopy. Applied Physics Letters, 116(6), Article 061601. https://doi.org/10.1063/1.5143950

Using atom beams to image the surface of samples in real space is an emerging technique that delivers unique contrast from delicate samples. Here, we explore the contrast that arises from multiple scattering of helium atoms, a specific process that p... Read More about Multiple scattering in scanning helium microscopy.

Engineering a Multilayered Skin Equivalent: The Importance of Endogenous Extracellular Matrix Maturation to Provide Robustness and Reproducibility (2019)
Book Chapter
Costello, L., Fullard, N., Roger, M., Bradbury, S., Dicolandrea, T., Isfort, R., …Przyborski, S. (2019). Engineering a Multilayered Skin Equivalent: The Importance of Endogenous Extracellular Matrix Maturation to Provide Robustness and Reproducibility. In S. Böttcher-Haberzeth, & T. Biedermann (Eds.), Skin Tissue Engineering (107-122). https://doi.org/10.1007/978-1-4939-9473-1_9

Bioengineering the Microanatomy of Human Skin (2019)
Journal Article
Roger, M., Fullard, N., Costello, L., Bradbury, S., Markiewicz, E., O'Reilly, S., …Przyborski, S. (2019). Bioengineering the Microanatomy of Human Skin. Journal of Anatomy, 234(4), 438-455. https://doi.org/10.1111/joa.12942

Recreating the structure of human tissues in the laboratory is valuable for fundamental research, testing interventions, and reducing the use of animals. Critical to the use of such technology is the ability to produce tissue models that accurately r... Read More about Bioengineering the Microanatomy of Human Skin.

An essential role for the Zn2+ transporter ZIP7 in B cell development (2019)
Journal Article
Anzilotti, C., Swan, D. J., Boisson, B., Deobagkar-Lele, M., Oliveira, C., Chabosseau, P., …Hambleton, S. (2019). An essential role for the Zn2+ transporter ZIP7 in B cell development. Nature Immunology, 20, 350-361. https://doi.org/10.1038/s41590-018-0295-8

Despite the known importance of zinc for human immunity, molecular insights into its roles have remained limited. Here we report a novel autosomal recessive disease characterized by absent B cells, agammaglobulinemia and early onset infections in fiv... Read More about An essential role for the Zn2+ transporter ZIP7 in B cell development.

Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa (2018)
Journal Article
Buskin, A., Zhu, L., Chichagova, V., Basu, B., Mozaffari-Jovin, S., Dolan, D., …Lako, M. (2018). Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa. Nature Communications, 9(1), Article 4234. https://doi.org/10.1038/s41467-018-06448-y

Mutations in pre-mRNA processing factors (PRPFs) cause autosomal-dominant retinitis pigmentosa (RP), but it is unclear why mutations in ubiquitously expressed genes cause non-syndromic retinal disease. Here, we generate transcriptome profiles from RP... Read More about Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa.

PyCoTools: a Python toolbox for COPASI (2018)
Journal Article
Welsh, C. M., Fullard, N., Proctor, C. J., Martinez-Guimera, A., Isfort, R. J., Bascom, C. C., …Shanley, D. P. (2018). PyCoTools: a Python toolbox for COPASI. Bioinformatics, 34(21), 3702-3710. https://doi.org/10.1093/bioinformatics/bty409

Motivation COPASI is an open source software package for constructing, simulating and analyzing dynamic models of biochemical networks. COPASI is primarily intended to be used with a graphical user interface but often it is desirable to be able to ac... Read More about PyCoTools: a Python toolbox for COPASI.

iPSC modeling of severe aplastic anemia reveals impaired differentiation and telomere shortening in blood progenitors (2018)
Journal Article
Melguizo-Sanchis, D., Xu, Y., Taheem, D., Yu, M., Tilgner, K., Barta, T., …Lako, M. (2018). iPSC modeling of severe aplastic anemia reveals impaired differentiation and telomere shortening in blood progenitors. Cell Death and Disease, 9(2), Article 128. https://doi.org/10.1038/s41419-017-0141-1

Aplastic Anemia (AA) is a bone marrow failure (BMF) disorder, resulting in bone marrow hypocellularity and peripheral pancytopenia. Severe aplastic anemia (SAA) is a subset of AA defined by a more severe phenotype. Although the immunological nature o... Read More about iPSC modeling of severe aplastic anemia reveals impaired differentiation and telomere shortening in blood progenitors.

An iPSC Patient Specific Model of CFH (Y402H) Polymorphism Displays Characteristic Features of AMD and Indicates a Beneficial Role for UV Light Exposure (2017)
Journal Article
Hallam, D., Collin, J., Bojic, S., Chichagova, V., Buskin, A., Xu, Y., …Lako, M. (2017). An iPSC Patient Specific Model of CFH (Y402H) Polymorphism Displays Characteristic Features of AMD and Indicates a Beneficial Role for UV Light Exposure. Stem Cells, 35(11), 2305-2320. https://doi.org/10.1002/stem.2708

Age related macular degeneration (AMD) is the most common cause of blindness, accounting for 8.7% of all blindness globally. Vision loss is caused ultimately by apoptosis of the retinal pigment epithelium (RPE) and overlying photoreceptors. Treatment... Read More about An iPSC Patient Specific Model of CFH (Y402H) Polymorphism Displays Characteristic Features of AMD and Indicates a Beneficial Role for UV Light Exposure.

MicroRNAs in the skin: role in development, homoeostasis and regeneration (2017)
Journal Article
Horsburgh, S., Fullard, N., Roger, M., Degnan, A., Todryk, S., Przyborski, S., & O’Reilly, S. (2017). MicroRNAs in the skin: role in development, homoeostasis and regeneration. Clinical Science, 131(15), 1923-1940. https://doi.org/10.1042/cs20170039

The skin is the largest organ of the integumentary system and possesses a vast number of functions. Due to the distinct layers of the skin and the variety of cells which populate each, a tightly regulated network of molecular signals control developm... Read More about MicroRNAs in the skin: role in development, homoeostasis and regeneration.

Systems modelling ageing: from single senescent cells to simple multi-cellular models (2017)
Journal Article
Martinez Guimera, A., Welsh, C., Dalle Pezze, P., Fullard, N., Nelson, G., Roger, M., …Shanley, D. (2017). Systems modelling ageing: from single senescent cells to simple multi-cellular models. Essays in Biochemistry, 61(3), 369-377. https://doi.org/10.1042/ebc20160087

Systems modelling has been successfully used to investigate several key molecular mechanisms of ageing. Modelling frameworks to allow integration of models and methods to enhance confidence in models are now well established. In this article, we disc... Read More about Systems modelling ageing: from single senescent cells to simple multi-cellular models.

Induced pluripotent stem cell modelling of HLHS underlines the contribution of dysfunctional NOTCH signalling to impaired cardiogenesis (2017)
Journal Article
Yang, C., Xu, Y., Yu, M., Lee, D., Alharti, S., Hellen, N., …Armstrong, L. (2017). Induced pluripotent stem cell modelling of HLHS underlines the contribution of dysfunctional NOTCH signalling to impaired cardiogenesis. Human Molecular Genetics, 26(16), 3031-3045. https://doi.org/10.1093/hmg/ddx140

Hypoplastic left heart syndrome (HLHS) is among the most severe forms of congenital heart disease. Although the consensus view is that reduced flow through the left heart during development is a key factor in the development of the condition, the mol... Read More about Induced pluripotent stem cell modelling of HLHS underlines the contribution of dysfunctional NOTCH signalling to impaired cardiogenesis.

Neurogenesis in Response to Synthetic Retinoids at Different Temporal Scales (2017)
Journal Article
Haffez, H., Khatib, T., McCaffery, P., Przyborski, S., Redfern, C., & Whiting, A. (2018). Neurogenesis in Response to Synthetic Retinoids at Different Temporal Scales. Molecular Neurobiology, 55(3), 1942-1950. https://doi.org/10.1007/s12035-017-0440-7

All-trans retinoic acid (ATRA) plays key roles in neurogenesis mediated by retinoic acid receptors (RARs). RARs are important targets for the therapeutic regulation of neurogenesis but effective drug development depends on modelling-based strategies... Read More about Neurogenesis in Response to Synthetic Retinoids at Different Temporal Scales.

Advanced cell culture technology for generation of in vivo-like tissue models (2017)
Journal Article
Przyborski, S. (2017). Advanced cell culture technology for generation of in vivo-like tissue models. International journal of health animal science & food safety, 4(1), https://doi.org/10.13130/2283-3927/8524

Human tissues are mostly composed of different cell types, that are often highly organised in relation to each other. Often cells are arranged in distinct layers that enable signalling and cell-to-cell interactions. Here we describe the application o... Read More about Advanced cell culture technology for generation of in vivo-like tissue models.

A robust and reproducible human pluripotent stem cell derived model of neurite outgrowth in a three-dimensional culture system and its application to study neurite inhibition (2016)
Journal Article
Clarke, K., Tams, D., Henderson, A., Roger, M., Whiting, A., & Przyborski, S. (2016). A robust and reproducible human pluripotent stem cell derived model of neurite outgrowth in a three-dimensional culture system and its application to study neurite inhibition. Neurochemistry International, 106, 74-84. https://doi.org/10.1016/j.neuint.2016.12.009

The inability of neurites to grow and restore neural connections is common to many neurological disorders, including trauma to the central nervous system and neurodegenerative diseases. Therefore, there is need for a robust and reproducible model of... Read More about A robust and reproducible human pluripotent stem cell derived model of neurite outgrowth in a three-dimensional culture system and its application to study neurite inhibition.

Histone demethylation and toll‐like receptor 8–dependent cross‐talk in monocytes promotes transdifferentiation of fibroblasts in systemic sclerosis via Fra‐2 (2016)
Journal Article
Ciechomska, M., O'Reilly, S., Przyborski, S., Oakley, F., Bogunia-Kubik, K., & van Laar, J. (2016). Histone demethylation and toll‐like receptor 8–dependent cross‐talk in monocytes promotes transdifferentiation of fibroblasts in systemic sclerosis via Fra‐2. Arthritis and Rheumatology, 68(6), 1493-1504. https://doi.org/10.1002/art.39602

Objectives: To investigate whether epigenetic changes can modulate monocytes to produce tissue-inhibitor of metalloproteinase-1 (TIMP-1) via Fra2 (AP-1 family member), a novel downstream mediator promoting fibrogenesis. Methods: AP-1 transcription fa... Read More about Histone demethylation and toll‐like receptor 8–dependent cross‐talk in monocytes promotes transdifferentiation of fibroblasts in systemic sclerosis via Fra‐2.

IL-13 mediates collagen deposition via STAT6 and microRNA-135b: a role for epigenetics (2016)
Journal Article
O’Reilly, S., Ciechomska, M., Fullard, N., Przyborski, S., & van Laar, J. (2016). IL-13 mediates collagen deposition via STAT6 and microRNA-135b: a role for epigenetics. Scientific Reports, 6, Article 25066. https://doi.org/10.1038/srep25066

Systemic sclerosis is an autoimmune connective tissue disease in which T cells play a prominent role. We and others have previously demonstrated a role for T cell-derived IL-13 in mediating the induction of collagen in dermal fibroblasts and that blo... Read More about IL-13 mediates collagen deposition via STAT6 and microRNA-135b: a role for epigenetics.

Advances in 3D cell culture technologies enabling tissue-like structures to be created in vitro (2015)
Journal Article
Knight, E., & Przyborski, S. (2015). Advances in 3D cell culture technologies enabling tissue-like structures to be created in vitro. Journal of Anatomy, 227(6), 746-756. https://doi.org/10.1111/joa.12257

Research in mammalian cell biology often relies on developing in vitro models to enable the growth of cells in the laboratory to investigate a specific biological mechanism or process under different test conditions. The quality of such models and ho... Read More about Advances in 3D cell culture technologies enabling tissue-like structures to be created in vitro.

Using Zinc Finger Nuclease Technology to Generate CRX-Reporter Human Embryonic Stem Cells as a Tool to Identify and Study the Emergence of Photoreceptors Precursors During Pluripotent Stem Cell Differentiation (2015)
Journal Article
Collin, J., Mellough, C., Dorgau, B., Przyborski, S., Moreno-Gimeno, I., & Lako, M. (2016). Using Zinc Finger Nuclease Technology to Generate CRX-Reporter Human Embryonic Stem Cells as a Tool to Identify and Study the Emergence of Photoreceptors Precursors During Pluripotent Stem Cell Differentiation. Stem Cells, 34(2), 311-321. https://doi.org/10.1002/stem.2240

The purpose of this study was to generate human embryonic stem cell (hESC) lines harboring the green fluorescent protein (GFP) reporter at the endogenous loci of the Cone-Rod Homeobox (CRX) gene, a key transcription factor in retinal development. Zin... Read More about Using Zinc Finger Nuclease Technology to Generate CRX-Reporter Human Embryonic Stem Cells as a Tool to Identify and Study the Emergence of Photoreceptors Precursors During Pluripotent Stem Cell Differentiation.

Neuronal-glial populations form functional networks in a biocompatible 3D scaffold (2015)
Journal Article
Smith, I., Haag, M., Ugbode, C., Tams, D., Rattray, M., Przyborski, S., …Whalley, B. (2015). Neuronal-glial populations form functional networks in a biocompatible 3D scaffold. Neuroscience Letters, 609, 198-202. https://doi.org/10.1016/j.neulet.2015.10.044

Monolayers of neurons and glia have been employed for decades as tools for the study of cellular physiology and as the basis for a variety of standard toxicological assays. A variety of three dimensional (3D) culture techniques have been developed wi... Read More about Neuronal-glial populations form functional networks in a biocompatible 3D scaffold.

A novel fully-humanised 3D skin equivalent to model early melanoma invasion (2015)
Journal Article
Hill, D., Robinson, N., Caley, M., Chen, M., O'Toole, E., Armstrong, J., …Lovat, P. (2015). A novel fully-humanised 3D skin equivalent to model early melanoma invasion. Molecular Cancer Therapeutics, 14(11), 2665-2673. https://doi.org/10.1158/1535-7163.mct-15-0394

Metastatic melanoma remains incurable, emphasizing the acute need for improved research models to investigate the underlying biologic mechanisms mediating tumor invasion and metastasis, and to develop more effective targeted therapies to improve clin... Read More about A novel fully-humanised 3D skin equivalent to model early melanoma invasion.

Fully biodegradable and biocompatible emulsion template polymer scaffolds by thiol-acrylate polymerisation of polycaprolactone macropolymers (2015)
Journal Article
Johnson, D., Langford, C., Didsbury, M., Lipp, B., Przyborski, S., & Cameron, N. (2015). Fully biodegradable and biocompatible emulsion template polymer scaffolds by thiol-acrylate polymerisation of polycaprolactone macropolymers. Polymer Chemistry, 6(41), 7256-7263. https://doi.org/10.1039/c5py00721f

The emulsion templating process offers a route to highly porous polymers with well-defined morphologies. This study describes the preparation of such porous polymers (polyHIPEs) via the photopolymerization of a multi-functional thiol and polycaprolac... Read More about Fully biodegradable and biocompatible emulsion template polymer scaffolds by thiol-acrylate polymerisation of polycaprolactone macropolymers.

An induced pluripotent stem cell model of hypoplastic left heart syndrome (HLHS) reveals multiple expression and functional differences in HLHS-derived cardiac myocytes (2014)
Journal Article
Jiang, Y., Habibollah, S., Tilgner, K., Collin, J., Barta, T., Al-Aama, J., …Armstrong, L. (2014). An induced pluripotent stem cell model of hypoplastic left heart syndrome (HLHS) reveals multiple expression and functional differences in HLHS-derived cardiac myocytes. Stem Cells Translational Medicine, 3(4), 416-423. https://doi.org/10.5966/sctm.2013-0105

Hypoplastic left heart syndrome (HLHS) is a serious congenital cardiovascular malformation resulting in hypoplasia or atresia of the left ventricle, ascending aorta, and aortic and mitral valves. Diminished flow through the left side of the heart is... Read More about An induced pluripotent stem cell model of hypoplastic left heart syndrome (HLHS) reveals multiple expression and functional differences in HLHS-derived cardiac myocytes.

Application of Synthetic Photostable Retinoids Induces Novel Limb and Facial Phenotypes During Chick Embryogenesis In Vivo (2014)
Journal Article
Lopez-Real, R., Budge, J., Marder, T., Whiting, A., Hunt, P., & Przyborski, S. (2014). Application of Synthetic Photostable Retinoids Induces Novel Limb and Facial Phenotypes During Chick Embryogenesis In Vivo. Journal of Anatomy, 224(4), 392-411. https://doi.org/10.1111/joa.12147

We have recently developed a range of synthetic retinoid analogues which include the compounds EC23 and EC19. They are stable on exposure to light and are predicted to be resistant to the normal metabolic processes involved in the inactivation of ret... Read More about Application of Synthetic Photostable Retinoids Induces Novel Limb and Facial Phenotypes During Chick Embryogenesis In Vivo.

A human iPSC model of Ligase IV deficiency reveals an important role for NHEJ-mediated-DSB repair in the survival and genomic stability of induced pluripotent stem cells and emerging haematopoietic progenitors. (2013)
Journal Article
Tilgner, K., Neganova, I., Moreno-Gimeno, I., Al-Aama, J., Burks, D., Yung, S., …Lako, M. (2013). A human iPSC model of Ligase IV deficiency reveals an important role for NHEJ-mediated-DSB repair in the survival and genomic stability of induced pluripotent stem cells and emerging haematopoietic progenitors. Cell Death & Differentiation, 20(8), 1089-1100. https://doi.org/10.1038/cdd.2013.44

Brief report: human pluripotent stem cell models of fanconi anemia deficiency reveal an important role for fanconi anemia proteins in cellular reprogramming and survival of hematopoietic progenitors (2013)
Journal Article
Yung, S., Tilgner, K., Ledran, M., Habibollah, S., Neganova, I., Singhapol, C., …Lako, M. (2013). Brief report: human pluripotent stem cell models of fanconi anemia deficiency reveal an important role for fanconi anemia proteins in cellular reprogramming and survival of hematopoietic progenitors. Stem Cells, 31(5), 1022-1029. https://doi.org/10.1002/stem.1308

Brief report: a human induced pluripotent stem cell model of cernunnos deficiency reveals an important role for XLF in the survival of the primitive hematopoietic progenitors. (2013)
Journal Article
Tilgner, K., Neganova, I., Singhapol, C., Saretzki, G., Al-Aama, J., Evans, J., …Lako, M. (2013). Brief report: a human induced pluripotent stem cell model of cernunnos deficiency reveals an important role for XLF in the survival of the primitive hematopoietic progenitors. Stem Cells, 31(9), 2015-2023. https://doi.org/10.1002/stem.1456

Design and biological evaluation of synthetic retinoids: Probing length vs. stability vs. activity (2013)
Journal Article
Clemens, G., Flower, K., Gardner, P., Henderson, A., Knowles, J., Marder, T., …Przyborski, S. (2013). Design and biological evaluation of synthetic retinoids: Probing length vs. stability vs. activity. Molecular bioSystems, 9(12), 3124-3134. https://doi.org/10.1039/c3mb70273a

All trans-retinoic acid (ATRA) is widely used to direct the differentiation of cultured stem cells. When exposed to the pluripotent human embryonal carcinoma (EC) stem cell line, TERA2.cl.SP12, ATRA induces ectoderm differentiation and the formation... Read More about Design and biological evaluation of synthetic retinoids: Probing length vs. stability vs. activity.

A novel model of urinary tract differentiation, tissue regeneration, and disease: reprogramming human prostate and bladder cells into induced pluripotent stem cells (2013)
Journal Article
Moad, M., Pal, D., Hepburn, A., Williamson, S., Wilson, L., Lako, M., …Heer, R. (2013). A novel model of urinary tract differentiation, tissue regeneration, and disease: reprogramming human prostate and bladder cells into induced pluripotent stem cells. European Urology, 64(5), 753-761. https://doi.org/10.1016/j.eururo.2013.03.054

Background: Primary culture and animal and cell-line models of prostate and bladder development have limitations in describing human biology, and novel strategies that describe the full spectrum of differentiation from foetal through to ageing tissue... Read More about A novel model of urinary tract differentiation, tissue regeneration, and disease: reprogramming human prostate and bladder cells into induced pluripotent stem cells.

Galactose-Functionalized PolyHIPE Scaffolds for Use in Routine Three Dimensional Culture of Mammalian Hepatocytes (2013)
Journal Article
Hayward, A., Eissa, A., Maltman, D., Przyborski, S., & Cameron, N. (2013). Galactose-Functionalized PolyHIPE Scaffolds for Use in Routine Three Dimensional Culture of Mammalian Hepatocytes. Biomacromolecules, 14(12), 4271-4277. https://doi.org/10.1021/bm401145x

Three-dimensional (3D) cell culture is regarded as a more physiologically relevant method of growing cells in the laboratory compared to traditional monolayer cultures. Recently, the application of polystyrene-based scaffolds produced using polyHIPE... Read More about Galactose-Functionalized PolyHIPE Scaffolds for Use in Routine Three Dimensional Culture of Mammalian Hepatocytes.

Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME (2013)
Journal Article
Godoy, P., Hewitt, N., Albrecht, U., Andersen, M., Ansari, N., Bhattacharya, S., …Hengstler, J. (2013). Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME. Archives of Toxicology, 87(8), 1315-1530. https://doi.org/10.1007/s00204-013-1078-5

This review encompasses the most important advances in liver functions and hepatotoxicity and analyzes which mechanisms can be studied in vitro. In a complex architecture of nested, zonated lobules, the liver consists of approximately 80 % hepatocyte... Read More about Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME.

Human mesenchymal stem cells creating an immunosuppressive environment and promote breast cancer in mice (2013)
Journal Article
Ljujic, B., Milovanovic, M., Volarevic, V., Murray, B., Bugarski, D., Przyborski, S., …Stojkovic, M. (2013). Human mesenchymal stem cells creating an immunosuppressive environment and promote breast cancer in mice. Scientific Reports, 3, Article 2298. https://doi.org/10.1038/srep02298

Human mesenchymal stem cells (hMSC) can home to tumor sites and promote tumor growth. The effects of hMSC on tumor growth are controversial and involvement of hMSC in tumor immunology has not been adequately addressed. Therefore, we investigated whet... Read More about Human mesenchymal stem cells creating an immunosuppressive environment and promote breast cancer in mice.

Brief Report: Human Pluripotent Stem Cell Models of Fanconi Anemia Deficiency Reveal an Important Role for Fanconi Anemia Proteins in Cellular Reprogramming and Survival of Hematopoietic Progenitors (2013)
Journal Article
Yung, S., Tilgner, K., Ledran, M., Habibollah, S., Neganova, I., Singhapol, C., …Lako, M. (2013). Brief Report: Human Pluripotent Stem Cell Models of Fanconi Anemia Deficiency Reveal an Important Role for Fanconi Anemia Proteins in Cellular Reprogramming and Survival of Hematopoietic Progenitors. Stem Cells, 31(5), 1022–1029. https://doi.org/10.1002/stem.1308

Fanconi anemia (FA) is a genomic instability disorder caused by mutations in genes involved in replication-dependant-repair and removal of DNA cross-links. Mouse models with targeted deletions of FA genes have been developed; however, none of these e... Read More about Brief Report: Human Pluripotent Stem Cell Models of Fanconi Anemia Deficiency Reveal an Important Role for Fanconi Anemia Proteins in Cellular Reprogramming and Survival of Hematopoietic Progenitors.

Synthesis and applications of 2,4-disubstituted thiazoles derivatives as small molecule modulators of cellular development (2013)
Journal Article
Zhou, G., Tams, D., Marder, T., Valentine, R., Whiting, A., & Przyborski, S. (2013). Synthesis and applications of 2,4-disubstituted thiazoles derivatives as small molecule modulators of cellular development. Organic and Biomolecular Chemistry, 11(14), 2323-2334. https://doi.org/10.1039/c3ob00005b

Understanding how the structure of molecules relates to their function and biological activity is essential in the development of new analogues with targeted activity. This is especially relevant in mediating developmental processes in mammalian cell... Read More about Synthesis and applications of 2,4-disubstituted thiazoles derivatives as small molecule modulators of cellular development.

The action of all-trans-retinoic acid (ATRA) and synthetic retinoid analogues (EC19 and EC23) on human pluripotent stem cells differentiation investigated using single cell infrared microspectroscopy (2013)
Journal Article
Clemens, G., Flower, K., Henderson, A., Whiting, A., Przyborski, S., Jimenez-Hernandez, M., …Gardner, P. (2013). The action of all-trans-retinoic acid (ATRA) and synthetic retinoid analogues (EC19 and EC23) on human pluripotent stem cells differentiation investigated using single cell infrared microspectroscopy. Molecular bioSystems, 9(4), 677-692. https://doi.org/10.1039/c3mb25505k

All trans-retinoic acid (ATRA) is widely used to direct the differentiation of cultured stem cells. When exposed to the pluripotent human embryonal carcinoma (EC) stem cell line, TERA2.cl.SP12, ATRA induces ectoderm differentiation and the formation... Read More about The action of all-trans-retinoic acid (ATRA) and synthetic retinoid analogues (EC19 and EC23) on human pluripotent stem cells differentiation investigated using single cell infrared microspectroscopy.

Top-Down Label-Free LC-MALDI analysis of the peptidome during neural progenitor cell differentiation reveals complexity in cytoskeletal protein dynamics and identifies progenitor cell markers (2012)
Journal Article
Maltman, D., Brand, S., Belau, E., Paape, R., Suckau, D., & Przyborski, S. (2012). Top-Down Label-Free LC-MALDI analysis of the peptidome during neural progenitor cell differentiation reveals complexity in cytoskeletal protein dynamics and identifies progenitor cell markers. Stem Cells, 599-611

Top-down label-free LC-MALDI analysis of the peptidome during neural progenitor cell differentiation reveals complexity in cytoskeletal protein dynamics and identifies progenitor cell markers (2011)
Journal Article
Maltman, D., Brand, S., Belau, E., Paape, R., Suckau, D., & Przyborski, S. (2011). Top-down label-free LC-MALDI analysis of the peptidome during neural progenitor cell differentiation reveals complexity in cytoskeletal protein dynamics and identifies progenitor cell markers. Proteomics, 11(20), 3992-4006. https://doi.org/10.1002/pmic.201100024

Three Dimensional Cell Culture (2008)
Journal Article
Carnachan, R., Bokhari, M., Cameron, N., Maatta, A., & Przyborski, S. (2008). Three Dimensional Cell Culture

Altered neurogenesis in Alzheimer's disease (2006)
Journal Article
Ziabreva, I., Perry, E., Perry, R., Minger, S., Ekonomou, A., Przyborski, S., & Ballard, C. (2006). Altered neurogenesis in Alzheimer's disease. Journal of Psychosomatic Research, 61, 311-316

Derivation of human embryonic stem cells from developing and arrested embryos (2006)
Journal Article
Zhang, X., Stojkovic, P., Przyborski, S., Cooke, M., Armstrong, L., Lako, M., & Stojkovic, M. (2006). Derivation of human embryonic stem cells from developing and arrested embryos. Stem Cells, 24(12), 2669–2676. https://doi.org/10.1634/stemcells.2006-0377

Human embryonic stem cells (hESC) hold huge promise in modern regenerative medicine, drug discovery, and as a model for studying early human development. However, usage of embryos and derivation of hESC for research and potential medical application... Read More about Derivation of human embryonic stem cells from developing and arrested embryos.

Formation of neurons by non-neural adult stem cells: potential mechanism implicates an artifact of growth in culture (2006)
Journal Article
Croft, A., & Przyborski, S. (2006). Formation of neurons by non-neural adult stem cells: potential mechanism implicates an artifact of growth in culture. Stem Cells, 24(8), 1841-1851. https://doi.org/10.1634/stemcells.2005-0609

Trans-differentiation is a mechanism proposed to explain how tissue-specific stem cells could generate cells of other organs, thus supporting the emerging concept of enhanced adult stem cell plasticity. Although spontaneous cell fusion rather than tr... Read More about Formation of neurons by non-neural adult stem cells: potential mechanism implicates an artifact of growth in culture.

Tailoring the morphology of emulsion-templated porous polymers (2006)
Journal Article
Carnachan, R., Bokhari, M., Przyborski, S., & Cameron, N. (2006). Tailoring the morphology of emulsion-templated porous polymers. Soft Matter, 2(7), 608-616. https://doi.org/10.1039/b603211g

Methods with which to tailor the morphology of polystyrene-based emulsion-templated (PolyHIPE) materials are presented. Increasing the temperature of the aqueous phase used to prepare the parent emulsion leads to an increase in average void and inter... Read More about Tailoring the morphology of emulsion-templated porous polymers.

Rewriteable DNA Microarrays (2006)
Journal Article
Schofield, W., McGettrick, J., Bradley, T., Badyal, J., & Przyborski, S. (2006). Rewriteable DNA Microarrays. Journal of the American Chemical Society, 128(7), 2280–2285. https://doi.org/10.1021/ja056367r

Thiol-terminated single-stranded deoxyribonucleic acids (ssDNA) can be immobilized onto pulsed plasma deposited poly(allylmercaptan) surfaces via disulfide bridge chemistry and are found to readily undergo nucleic acid hybridization. Unlike other met... Read More about Rewriteable DNA Microarrays.

Porous polymers by emulsion templating. (2005)
Journal Article
Barbetta, A., Carnachan, R., Smith, K., Zhao, C., Cameron, N., Kataky, R., …Swan, M. (2005). Porous polymers by emulsion templating. Macromolecular Symposia, 226(1), 203-212. https://doi.org/10.1002/masy.200550819

Highly porous and permeable polymers are produced by polymerisation of the continuous phase of high internal phase emulsions (HIPEs). The morphology and properties of the resulting PolyHIPE materials can be varied, allowing the materials to be optimi... Read More about Porous polymers by emulsion templating..

Enhanced neurite outgrowth by human neurons grown on solid three-dimensional scaffolds. (2004)
Journal Article
Hayman, M., Smith, K., Cameron, N., & Przyborski, S. (2004). Enhanced neurite outgrowth by human neurons grown on solid three-dimensional scaffolds. Biochemical and Biophysical Research Communications, 314(2), 483-488. https://doi.org/10.1016/j.bbrc.2003.12.135

Growing and differentiating human stem cells in vitro can provide access to study the molecular mechanisms that control cellular development in a manner pertinent to human embryogenesis. To fully understand such processes, however, it is important to... Read More about Enhanced neurite outgrowth by human neurons grown on solid three-dimensional scaffolds..

Transcriptional profiling of neuronal differentiation by human embryonal carcinoma stem cells in vitro (2003)
Journal Article
Przyborski, S., Smith, S., & Wood, A. (2003). Transcriptional profiling of neuronal differentiation by human embryonal carcinoma stem cells in vitro. Stem Cells, 21(4), 459-471. https://doi.org/10.1634/stemcells.21-4-459

Pluripotent stem cell lines can be induced to differentiate into a range of somatic cell types in response to various stimuli. Such cell-based systems provide powerful tools for the investigation of molecules that modulate cellular development. For i... Read More about Transcriptional profiling of neuronal differentiation by human embryonal carcinoma stem cells in vitro.

Manipulation of human pluripotent embryonal carcinoma stem cells and the development of neural subtypes (2003)
Journal Article
Stewart, R., Christie, V., & Przyborski, S. (2003). Manipulation of human pluripotent embryonal carcinoma stem cells and the development of neural subtypes. Stem Cells, 21(3), 248-256. https://doi.org/10.1634/stemcells.21-3-248

There are few reliable cell systems available to study the process of human neural development. Embryonal carcinoma (EC) cells are pluripotent stem cells derived from teratocarcinomas and offer a robust culture system to research cell differentiation... Read More about Manipulation of human pluripotent embryonal carcinoma stem cells and the development of neural subtypes.

Isolation of human embryonal carcinoma stem cells by immuno-magnetic sorting (2001)
Journal Article
Przyborski, S. (2001). Isolation of human embryonal carcinoma stem cells by immuno-magnetic sorting. Stem Cells, 19(6), 500-504. https://doi.org/10.1634/stemcells.19-6-500

Embryonal carcinoma cells are pluripotent stem cells derived from germ cell tumors and can be used to study cell differentiation in vitro. This report describes an approach designed to isolate pluripotent stem cells from primary/parent stock cultures... Read More about Isolation of human embryonal carcinoma stem cells by immuno-magnetic sorting.