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A novel function for the MAP kinase SMA-5 in intestinal tube stability (2016)
Journal Article
Geisler, F., Gerhardus, H., Carberry, K., Davis, W., Jorgensen, E., Richardson, C., …Leube, R. E. (2016). A novel function for the MAP kinase SMA-5 in intestinal tube stability. Molecular Biology of the Cell, 27(24), 3855-3868. https://doi.org/10.1091/mbc.e16-02-0099

Intermediate filaments are major cytoskeletal components whose assembly into complex networks and isotype-specific functions are still largely unknown. Caenorhabditis elegans provides an excellent model system to study intermediate filament organizat... Read More about A novel function for the MAP kinase SMA-5 in intestinal tube stability.

Immunoelectron Microscopy of Cryofixed Freeze-Substituted Yeast (2016)
Book Chapter
Fišerová, J., Richardson, C., & Goldberg, M. W. (2016). Immunoelectron Microscopy of Cryofixed Freeze-Substituted Yeast. In S. D. Schwartzbach, O. Skalli, & T. Schikorski (Eds.), High-Resolution Imaging of Cellular Proteins (243-258). Humana Press. https://doi.org/10.1007/978-1-4939-6352-2_15

Immunolabeling electron microscopy is a challenging technique with demands for perfect ultrastructural and antigen preservation. High-pressure freezing offers an excellent way to fix cellular structure. However, its use for immunolabeling has remaine... Read More about Immunoelectron Microscopy of Cryofixed Freeze-Substituted Yeast.

Arabidopsis NAP1 regulates the formation of autophagosomes (2016)
Journal Article
Wang, P., Richardson, C., Hawes, C., & Hussey, P. (2016). Arabidopsis NAP1 regulates the formation of autophagosomes. Current Biology, 26(15), 2060-2069. https://doi.org/10.1016/j.cub.2016.06.008

The SCAR/WAVE complex is required for ARP2/3-mediated actin nucleation, and these complexes are highly conserved in plants and animals [1 and 2]. Proteins from the SCAR/WAVE complex have been found to be membrane associated in plants [3]. Using fluor... Read More about Arabidopsis NAP1 regulates the formation of autophagosomes.

Plant VAP27 proteins: domain characterization, intracellular localization and role in plant development (2016)
Journal Article
Wang, P., Richardson, C., Hawkins, T. J., Sparkes, I., Hawes, C., & Hussey, P. J. (2016). Plant VAP27 proteins: domain characterization, intracellular localization and role in plant development. New Phytologist, 210(4), 1311-1326. https://doi.org/10.1111/nph.13857

The endoplasmic reticulum (ER) is connected to the plasma membrane (PM) through the plant-specific NETWORKED protein, NET3C, and phylogenetically conserved vesicle-associated membrane protein-associated proteins (VAPs). Ten VAP homologues (VAP27-1 to... Read More about Plant VAP27 proteins: domain characterization, intracellular localization and role in plant development.