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A novel function for the MAP kinase SMA-5 in intestinal tube stability

Geisler, Florian; Gerhardus, Harald; Carberry, Katrin; Davis, Wayne; Jorgensen, Erik; Richardson, Christine; Bossinger, Olaf; Leube, Rudolf E.

A novel function for the MAP kinase SMA-5 in intestinal tube stability Thumbnail


Authors

Florian Geisler

Harald Gerhardus

Katrin Carberry

Wayne Davis

Erik Jorgensen

Christine Richardson

Olaf Bossinger

Rudolf E. Leube



Abstract

Intermediate filaments are major cytoskeletal components whose assembly into complex networks and isotype-specific functions are still largely unknown. Caenorhabditis elegans provides an excellent model system to study intermediate filament organization and function in vivo. Its intestinal intermediate filaments localize exclusively to the endotube, a circumferential sheet just below the actin-based terminal web. A genetic screen for defects in the organization of intermediate filaments identified a mutation in the catalytic domain of the MAP kinase 7 orthologue sma-5(kc1). In sma-5(kc1) mutants, pockets of lumen penetrate the cytoplasm of the intestinal cells. These membrane hernias increase over time without affecting epithelial integrity and polarity. A more pronounced phenotype was observed in the deletion allele sma-5(n678) and in intestine-specific sma-5(RNAi). Besides reduced body length, an increased time of development, reduced brood size, and reduced life span were observed in the mutants, indicating compromised food uptake. Ultrastructural analyses revealed that the luminal pockets include the subapical cytoskeleton and coincide with local thinning and gaps in the endotube that are often enlarged in other regions. Increased intermediate filament phosphorylation was detected by two-dimensional immunoblotting, suggesting that loss of SMA-5 function leads to reduced intestinal tube stability due to altered intermediate filament network phosphorylation.

Citation

Geisler, F., Gerhardus, H., Carberry, K., Davis, W., Jorgensen, E., Richardson, C., …Leube, R. E. (2016). A novel function for the MAP kinase SMA-5 in intestinal tube stability. Molecular Biology of the Cell, 27(24), 3855-3868. https://doi.org/10.1091/mbc.e16-02-0099

Journal Article Type Article
Acceptance Date Oct 5, 2016
Online Publication Date Oct 12, 2016
Publication Date Dec 1, 2016
Deposit Date Oct 13, 2016
Publicly Available Date Dec 16, 2016
Journal Molecular biology of the cell
Publisher American Society for Cell Biology
Peer Reviewed Peer Reviewed
Volume 27
Issue 24
Pages 3855-3868
DOI https://doi.org/10.1091/mbc.e16-02-0099
Public URL https://durham-repository.worktribe.com/output/1372800

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Publisher Licence URL
http://creativecommons.org/licenses/by-nc-sa/4.0/

Copyright Statement
© 2016 Geisler et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available
to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).





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