All Outputs (67)

Recent advances in proteomic workflows to interrogate the SUMO ylome in plants (2025)
Journal Article
Ingole, K. D., Alekseeva, E., Lilley, K. S., & Sadanandom, A. (online). Recent advances in proteomic workflows to interrogate the SUMO ylome in plants. New Phytologist, https://doi.org/10.1111/nph.70176

Summary: Protein posttranslational modifications (PTMs) are vital for regulating protein functions. SUMOylation, a PTM essential for plant survival, involves attaching a Small Ubiquitin‐like MOdifier (SUMO) to lysine residues of target proteins. SUMO... Read More about Recent advances in proteomic workflows to interrogate the SUMO ylome in plants.

Single-cell transcriptomics reveal how root tissues adapt to soil stress (2025)
Journal Article
Zhu, M., Hsu, C. W., Peralta Ogorek, L. L., Taylor, I. W., La Cavera, S., Oliveira, D. M., Verma, L., Mehra, P., Mijar, M., Sadanandom, A., Perez-Cota, F., Boerjan, W., Nolan, T. M., Bennett, M. J., Benfey, P. N., & Pandey, B. K. (online). Single-cell transcriptomics reveal how root tissues adapt to soil stress. Nature, https://doi.org/10.1038/s41586-025-08941-z

Land plants thrive in soils showing vastly different properties and environmental stresses1. Root systems can adapt to contrasting soil conditions and stresses, yet how their responses are programmed at the individual cell scale remains unclear. Usin... Read More about Single-cell transcriptomics reveal how root tissues adapt to soil stress.

Charting the evolutionary path of the SUMO modification system in plants reveals molecular hardwiring of development to stress adaptation (2024)
Journal Article
Ghosh, S., Mellado Sanchez, M., Sue-Ob, K., Roy, D., Jones, A., Blazquez, M. A., & Sadanandom, A. (2024). Charting the evolutionary path of the SUMO modification system in plants reveals molecular hardwiring of development to stress adaptation. The Plant Cell, 36(9), 3131-3144. https://doi.org/10.1093/plcell/koae192

SUMO modification is part of the spectrum of Ubiquitin-like (UBL) systems that give rise to proteoform complexity through post-translational modifications (PTMs). Proteoforms are essential modifiers of cell signaling for plant adaptation to changing... Read More about Charting the evolutionary path of the SUMO modification system in plants reveals molecular hardwiring of development to stress adaptation.

Focus on proteolysis. (2024)
Journal Article
Eckardt, N. A., Genschik, P., Jiang, L., Li, X., Otegui, M. S., Sadanandom, A., Spoel, S., van Wijk, K. J., & Weijers, D. (2024). Focus on proteolysis. The Plant Cell, 36(9), 2929–2930. https://doi.org/10.1093/plcell/koae182

Inheritance of Resistance to Chickpea Fusarium Wilt Disease ( Fusarium oxysporum f. sp. ciceris Race 2) in a Wide-Cross Cicer arietinum × Cicer reticulatum Mapping Family (2024)
Journal Article
Lakmes, A., Jhar, A., Sadanandom, A., Brennan, A. C., & Kahriman, A. (2024). Inheritance of Resistance to Chickpea Fusarium Wilt Disease ( Fusarium oxysporum f. sp. ciceris Race 2) in a Wide-Cross Cicer arietinum × Cicer reticulatum Mapping Family. Genes, 15(6), Article 819. https://doi.org/10.3390/genes15060819

Chickpea (Cicer arietinum) is a major food legume providing high quality nutrition, especially in developing regions. Chickpea wilt (Fusarium oxysporum f. sp. ciceris) causes significant annual losses. Integrated disease management of Fusarium wilt i... Read More about Inheritance of Resistance to Chickpea Fusarium Wilt Disease ( Fusarium oxysporum f. sp. ciceris Race 2) in a Wide-Cross Cicer arietinum × Cicer reticulatum Mapping Family.

SUMO protease FUG1, histone reader AL3 and chromodomain protein LHP1 are integral to repeat expansion-induced gene silencing in Arabidopsis thaliana. (2024)
Journal Article
Sureshkumar, S., Bandaranayake, C., Lv, J., Dent, C. I., Bhagat, P. K., Mukherjee, S., …Balasubramanian, S. (2024). SUMO protease FUG1, histone reader AL3 and chromodomain protein LHP1 are integral to repeat expansion-induced gene silencing in Arabidopsis thaliana. Nature Plants, 10, 749-759. https://doi.org/10.1038/s41477-024-01672-5

Epigenetic gene silencing induced by expanded repeats can cause diverse phenotypes ranging from severe growth defects in plants to genetic diseases such as Friedreich's ataxia in humans. The molecular mechanisms underlying repeat expansion-induced ep... Read More about SUMO protease FUG1, histone reader AL3 and chromodomain protein LHP1 are integral to repeat expansion-induced gene silencing in Arabidopsis thaliana..

SUMOylation of OsPSTOL1 is essential for regulating phosphate starvation responses in rice and Arabidopsis (2024)
Journal Article
Mukkawar, V., Roy, D., Sue-ob, K., Jones, A., Zhang, C., Kumar Bhagat, P., Kakkunnath, S. M., Heuer, S., & Sadanandom, A. (2024). SUMOylation of OsPSTOL1 is essential for regulating phosphate starvation responses in rice and Arabidopsis. Frontiers in Plant Science, 15, Article 1274610. https://doi.org/10.3389/fpls.2024.1274610

Although rice is one of the main sources of calories for most of the world, nearly 60% of rice is grown in soils that are low in phosphorus especially in Asia and Africa. Given the limitations of bioavailable inorganic phosphate (Pi) in soils, it is... Read More about SUMOylation of OsPSTOL1 is essential for regulating phosphate starvation responses in rice and Arabidopsis.

SUMO/deSUMOylation of the BRI1 brassinosteroid receptor modulates plant growth responses to temperature (2023)
Journal Article
Naranjo-Arcos, M., Srivastava, M., Deligne, F., Bhagat, P. K., Mansi, M., Sadanandom, A., & Vert, G. (2023). SUMO/deSUMOylation of the BRI1 brassinosteroid receptor modulates plant growth responses to temperature. Proceedings of the National Academy of Sciences, 120(4), Article 2217255120. https://doi.org/10.1073/pnas.2217255120

Brassinosteroids (BRs) are a class of steroid molecules perceived at the cell surface that act as plant hormones. The BR receptor BRASSINOSTEROID INSENSITIVE1 (BRI1) offers a model to understand receptor-mediated signaling in plants and the role of p... Read More about SUMO/deSUMOylation of the BRI1 brassinosteroid receptor modulates plant growth responses to temperature.

Understanding SUMO-mediated adaptive responses in plants to improve crop productivity (2022)
Journal Article
Clark, L., Sue-Ob, K., Mukkawar, V., Jones, A., & Sadanandom, A. (2022). Understanding SUMO-mediated adaptive responses in plants to improve crop productivity. Essays in Biochemistry, 66(2), 155-168. https://doi.org/10.1042/ebc20210068

The response to abiotic and biotic stresses in plants and crops is considered a multifaceted process. Due to their sessile nature, plants have evolved unique mechanisms to ensure that developmental plasticity remains during their life cycle. Among th... Read More about Understanding SUMO-mediated adaptive responses in plants to improve crop productivity.

The conjugation of SUMO to the transcription factor MYC2 functions in blue light-mediated seedling development in Arabidopsis (2022)
Journal Article
Srivastava, M., Srivastava, A. K., Roy, D., Mansi, M., Gough, C., Bhagat, P. K., Zhang, C., & Sadanandom, A. (2022). The conjugation of SUMO to the transcription factor MYC2 functions in blue light-mediated seedling development in Arabidopsis. The Plant Cell, 34(8), 2892-2906. https://doi.org/10.1093/plcell/koac142

A key function of photoreceptor signaling is the coordinated regulation of a large number of genes to optimize plant growth and development. The basic helix loop helix (bHLH) transcription factor MYC2 is crucial for regulating gene expression in Arab... Read More about The conjugation of SUMO to the transcription factor MYC2 functions in blue light-mediated seedling development in Arabidopsis.

TaWRKY10 transcription factor is a novel Jasmonic Acid signalling regulator involved in immunity against Septoria tritici blotch disease in wheat (2021)
Journal Article
Campanaro, A., Srivastava, A., Zhang, C., Lee, J., Millyard, L., Gatehouse, A., Byrne, E., & Sadanandom, A. (2021). TaWRKY10 transcription factor is a novel Jasmonic Acid signalling regulator involved in immunity against Septoria tritici blotch disease in wheat. Plant Pathology, 70(6), 1397-1408. https://doi.org/10.1111/ppa.13388

Septoria tritici blotch (STB), caused by the fungus Zymoseptoria tritici, is currently the main threat to worldwide wheat production in temperate climates. Understanding the genetic mechanisms that underpin the Z. tritici–wheat interaction will be cr... Read More about TaWRKY10 transcription factor is a novel Jasmonic Acid signalling regulator involved in immunity against Septoria tritici blotch disease in wheat.

SUMO enables substrate selectivity by mitogen-activated protein kinases to regulate immunity in plants (2021)
Journal Article
Verma, V., Srivastava, A. K., Gough, C., Campanaro, A., Srivastava, M., Morrell, R., Joyce, J., Bailey, M., Zhang, C., Krysan, P. J., & Sadanandom, A. (2021). SUMO enables substrate selectivity by mitogen-activated protein kinases to regulate immunity in plants. Proceedings of the National Academy of Sciences, 118(10), Article e2021351118. https://doi.org/10.1073/pnas.2021351118

The versatility of mitogen-activated protein kinases (MAPKs) in translating exogenous and endogenous stimuli into appropriate cellular responses depends on its substrate specificity. In animals, several mechanisms have been proposed about how MAPKs m... Read More about SUMO enables substrate selectivity by mitogen-activated protein kinases to regulate immunity in plants.

SUMO mediated regulation of transcription factors as a mechanism for transducing environmental cues into cellular signaling in plants (2021)
Journal Article
Roy, D., & Sadanandom, A. (2021). SUMO mediated regulation of transcription factors as a mechanism for transducing environmental cues into cellular signaling in plants. Cellular and Molecular Life Sciences, 78(6), 2641-2664. https://doi.org/10.1007/s00018-020-03723-4

Across all species, transcription factors (TFs) are the most frequent targets of SUMOylation. The effect of SUMO conjugation on the functions of transcription factors has been extensively studied in animal systems, with over 200 transcription factors... Read More about SUMO mediated regulation of transcription factors as a mechanism for transducing environmental cues into cellular signaling in plants.

Towards understanding the multifaceted role of SUMOylation in plant growth and development (2020)
Journal Article
Srivastava, M., Sadanandom, A., & Srivastava, A. K. (2020). Towards understanding the multifaceted role of SUMOylation in plant growth and development. Physiologia Plantarum, 171(1), 77-85. https://doi.org/10.1111/ppl.13204

Post‐translational modifications (PTMs) play a critical role in regulating plant growth and development through the modulation of protein functionality and its interaction with its partners. Analysis of the functional implication of PTMs on plant cel... Read More about Towards understanding the multifaceted role of SUMOylation in plant growth and development.

HEARTBREAK Controls Post-translational Modification of INDEHISCENT to Regulate Fruit Morphology in Capsella (2020)
Journal Article
Dong, Y., Majda, M., Šimura, J., Horvath, R., Srivastava, A. K., Łangowski, Ł., Eldridge, T., Stacey, N., Slotte, T., Sadanandom, A., Ljung, K., Smith, R. S., & Østergaard, L. (2020). HEARTBREAK Controls Post-translational Modification of INDEHISCENT to Regulate Fruit Morphology in Capsella. Current Biology, 30(19), 3880-3888.e5. https://doi.org/10.1016/j.cub.2020.07.055

Morphological variation is the basis of natural diversity and adaptation. For example, angiosperms (flowering plants) evolved during the Cretaceous period more than 100 mya and quickly colonized terrestrial habitats [1]. A major reason for their asto... Read More about HEARTBREAK Controls Post-translational Modification of INDEHISCENT to Regulate Fruit Morphology in Capsella.

SUMO Conjugation to BZR1 Enables Brassinosteroid Signaling to Integrate Environmental Cues to Shape Plant Growth (2020)
Journal Article
Srivastava, M., Srivastava, A. K., Orosa-Puente, B., Campanaro, A., Zhang, C., & Sadanandom, A. (2020). SUMO Conjugation to BZR1 Enables Brassinosteroid Signaling to Integrate Environmental Cues to Shape Plant Growth. Current Biology, 30(8), 1423.e3-1423.e3. https://doi.org/10.1016/j.cub.2020.01.089

Brassinosteroids (BRs) play crucial roles in plant development, but little is known of mechanisms that integrate environmental cues into BR signaling. Conjugation to the small ubiquitin-like modifier (SUMO) is emerging as an important mechanism to tr... Read More about SUMO Conjugation to BZR1 Enables Brassinosteroid Signaling to Integrate Environmental Cues to Shape Plant Growth.

Identification of Transgene-Free CRISPR-Edited Plants of Rice, Tomato, and Arabidopsis by Monitoring DsRED Fluorescence in Dry Seeds (2019)
Journal Article
Aliaga-Franco, N., Zhang, C., Presa, S., Srivastava, A. K., Granell, A., Alabadí, D., Sadanandom, A., Blázquez, M. A., & Minguet, E. G. (2019). Identification of Transgene-Free CRISPR-Edited Plants of Rice, Tomato, and Arabidopsis by Monitoring DsRED Fluorescence in Dry Seeds. Frontiers in Plant Science, 10, Article 1150. https://doi.org/10.3389/fpls.2019.01150

Efficient elimination of the editing machinery remains a challenge in plant biotechnology after genome editing to minimize the probability of off-target mutations, but it is also important to deliver end users with edited plants free of foreign DNA.... Read More about Identification of Transgene-Free CRISPR-Edited Plants of Rice, Tomato, and Arabidopsis by Monitoring DsRED Fluorescence in Dry Seeds.

Dealing With Stress: A Review of Plant SUMO Proteases (2019)
Journal Article
Morrell, R., & Sadanandom, A. (2019). Dealing With Stress: A Review of Plant SUMO Proteases. Frontiers in Plant Science, 10, Article 1122. https://doi.org/10.3389/fpls.2019.01122

The SUMO system is a rapid dynamic post-translational mechanism employed by eukaryotic cells to respond to stress. Plant cells experience hyperSUMOylation of substrates in response to stresses such as heat, ethanol, and drought. Many SUMOylated prote... Read More about Dealing With Stress: A Review of Plant SUMO Proteases.

Root branching toward water involves posttranslational modification of transcription factor ARF7 (2018)
Journal Article
Orosa-Puente, B., Leftley, N., von Wangenheim, D., Banda, J., Srivastava, A. K., Hill, K., Truskina, J., Bhosale, R., Morris, E., Srivastava, M., Kümpers, B., Goh, T., Fukaki, H., Vermeer, J. E., Vernoux, T., Dinneny, J. R., French, A. P., Bishopp, A., Sadanandom, A., & Bennett, M. J. (2018). Root branching toward water involves posttranslational modification of transcription factor ARF7. Science, 362(6421), 1407-1410. https://doi.org/10.1126/science.aau3956

Plants adapt to heterogeneous soil conditions by altering their root architecture. For example, roots branch when in contact with water using the hydropatterning response. We report that hydropatterning is dependent on auxin response factor ARF7. Thi... Read More about Root branching toward water involves posttranslational modification of transcription factor ARF7.

SUMO conjugation to the pattern recognition receptor FLS2 triggers intracellular signalling in plant innate immunity (2018)
Journal Article
Orosa, B., Yates, G., Verma, V., Srivastava, A. K., Srivastava, M., Campanaro, A., De Vega, D., Fernandes, A., Zhang, C., Lee, J., Bennett, M. J., & Sadanandom, A. (2018). SUMO conjugation to the pattern recognition receptor FLS2 triggers intracellular signalling in plant innate immunity. Nature Communications, 9(1), Article 5185. https://doi.org/10.1038/s41467-018-07696-8

Detection of conserved microbial patterns by host cell surface pattern recognition receptors (PRRs) activates innate immunity. The FLAGELLIN-SENSITIVE 2 (FLS2) receptor perceives bacterial flagellin and recruits another PRR, BAK1 and the cytoplasmic-... Read More about SUMO conjugation to the pattern recognition receptor FLS2 triggers intracellular signalling in plant innate immunity.