Inheritance of Resistance to Chickpea Fusarium Wilt Disease ( Fusarium oxysporum f. sp. ciceris Race 2) in a Wide-Cross Cicer arietinum × Cicer reticulatum Mapping Family

Lakmes, Abdulkarim; Jhar, Abdullah; Sadanandom, Ari; Brennan, Adrian Christopher; Kahriman, Abdullah

Abstract

Chickpea (Cicer arietinum) is a major food legume providing high quality nutrition, especially in developing regions. Chickpea wilt (Fusarium oxysporum f. sp. ciceris) causes significant annual losses. Integrated disease management of Fusarium wilt is supported by resistant varieties. Relatively few resistance genes are known so there is value in exploring genetic resources in chickpea wild relatives. This study investigates the inheritance of Fusarium wilt resistance (race 2) in recombinant inbred lines (RILs) from a cross between a cultivated susceptible chickpea variety (Gokce) and a wild resistant Cicer reticulatum line (Kayat-077). RILs, parents, resistant and susceptible tester lines were twice grown in the greenhouse with inoculation and disease symptoms scored. DNA was extracted from dried leaves and individuals were single nucleotide polymorphism (SNP) genotyped. SNPs were placed on the reference chickpea genome and quantitative trait locus (QTL) mapping was performed. Significant QTL regions were examined using PulseDB to identify candidate genes. The results showed the segregation of Fusarium wilt resistance conforming to a single gene inheritance. One significant QTL was found at the start of chromosome 8, containing 138 genes, three of which were disease-resistance candidates for chickpea breeding.

Citation

Lakmes, A., Jhar, A., Sadanandom, A., Brennan, A. C., & Kahriman, A. (2024). Inheritance of Resistance to Chickpea Fusarium Wilt Disease ( Fusarium oxysporum f. sp. ciceris Race 2) in a Wide-Cross Cicer arietinum × Cicer reticulatum Mapping Family. Genes, 15(6), Article 819. https://doi.org/10.3390/genes15060819

• Journal Article Type: Article
• Acceptance Date: Jun 17, 2024
• Online Publication Date: Jun 20, 2024
• Publication Date: Jul 8, 2024
• Deposit Date: Jul 18, 2024
• Publicly Available Date: Jul 18, 2024
• Journal: Genes
• Electronic ISSN: 2073-4425
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 15
• Issue: 6
• Article Number: 819
• DOI: https://doi.org/10.3390/genes15060819
• Keywords: plant pathogen, monogenic inheritance, legume, disease phenotyping, crop wild relative, quantitative trait locus, wilt resistance
• Public URL: https://durham-repository.worktribe.com/output/2526075