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TaWRKY10 transcription factor is a novel Jasmonic Acid signalling regulator involved in immunity against Septoria tritici blotch disease in wheat

Campanaro, A.; Srivastava, A.K.; Zhang, C.; Lee, J.; Millyard, L.; Gatehouse, A.M.R.; Byrne, E.; Sadanandom, A.

TaWRKY10 transcription factor is a novel Jasmonic Acid signalling regulator involved in immunity against Septoria tritici blotch disease in wheat Thumbnail


Authors

A. Campanaro

A.K. Srivastava

J. Lee

L. Millyard

A.M.R. Gatehouse

E. Byrne



Abstract

Septoria tritici blotch (STB), caused by the fungus Zymoseptoria tritici, is currently the main threat to worldwide wheat production in temperate climates. Understanding the genetic mechanisms that underpin the Z. tritici–wheat interaction will be crucial for generating new control strategies against STB. Plant hormones are essential regulators of growth and immunity. In particular, jasmonic acid (JA) plays a central role in defence against necrotrophic plant pathogens, but its role in mediating immunity against Z. tritici is largely unknown. Here we identify the transcription factor TaWRKY10 that is taxonomically restricted to the grass subfamily Pooideae as a novel regulator of JA responses in wheat. We demonstrate by using virus-induced gene silencing that silencing TaWRKY10 leads to increased resistance against Z. tritici with an earlier onset of necrotic symptoms, but with reduced pathogen sporulation. Wheat plants silenced for TaWRKY10 show an up-regulated JA response. Transcriptional profiling of TaWRKY10 knock-down plants indicates that it is a negative regulator of the JA receptor TaCOI1 gene expression. Our data indicate that TaWRKY10 down-regulates JA responses, contributing to the susceptibility of wheat to Z. tritici. We postulate that manipulating TaWRKY10 may provide a strategy to boost STB resistance in wheat.

Citation

Campanaro, A., Srivastava, A., Zhang, C., Lee, J., Millyard, L., Gatehouse, A., …Sadanandom, A. (2021). TaWRKY10 transcription factor is a novel Jasmonic Acid signalling regulator involved in immunity against Septoria tritici blotch disease in wheat. Plant Pathology, 70(6), 1397-1408. https://doi.org/10.1111/ppa.13388

Journal Article Type Article
Acceptance Date Apr 8, 2021
Online Publication Date Apr 22, 2021
Publication Date Jul 22, 2021
Deposit Date Apr 29, 2021
Publicly Available Date Aug 20, 2021
Journal Plant Pathology
Print ISSN 0032-0862
Electronic ISSN 1365-3059
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 70
Issue 6
Pages 1397-1408
DOI https://doi.org/10.1111/ppa.13388

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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
© 2021 The Authors. Plant Pathology published by John Wiley & Sons Ltd on behalf of British Society for Plant Pathology.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.





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