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SUMOylation of OsPSTOL1 is essential for regulating phosphate starvation responses in rice and Arabidopsis

Mukkawar, Vaishnavi; Roy, Dipan; Sue-ob, Kawinnat; Jones, Andrew; Zhang, Cunjin; Kumar Bhagat, Prakash; Kakkunnath, Sumesh M.; Heuer, Sigrid; Sadanandom, Ari

SUMOylation of OsPSTOL1 is essential for regulating phosphate starvation responses in rice and Arabidopsis Thumbnail


Authors

Dr Dipan Roy dipan.roy@durham.ac.uk
Post Doctoral Research Associate

Kawinnat Sue-ob

Andrew Jones

Sigrid Heuer



Abstract

Although rice is one of the main sources of calories for most of the world, nearly 60% of rice is grown in soils that are low in phosphorus especially in Asia and Africa. Given the limitations of bioavailable inorganic phosphate (Pi) in soils, it is important to develop crops tolerant to low phosphate in order to boost food security. Due to the immobile nature of Pi, plants have developed complex molecular signalling pathways that allow them to discern changes in Pi concentrations in the environment and adapt their growth and development. Recently, in rice, it was shown that a specific serine–threonine kinase known as Phosphorus-starvation tolerance 1 (PSTOL1) is important for conferring low phosphate tolerance in rice. Nonetheless, knowledge about the mechanism underpinning PSTOL1 activity in conferring low Pi tolerance is very limited in rice. Post-translation modifications (PTMs) play an important role in plants in providing a conduit to detect changes in the environment and influence molecular signalling pathways to adapt growth and development. In recent years, the PTM SUMOylation has been shown to be critical for plant growth and development. It is known that plants experience hyperSUMOylation of target proteins during phosphate starvation. Here, we demonstrate that PSTOL1 is SUMOylated in planta, and this affects its phosphorylation activity. Furthermore, we also provide new evidence for the role of SUMOylation in regulating PSTOL1 activity in plant responses to Pi starvation in rice and Arabidopsis. Our data indicated that overexpression of the non-SUMOylatable version of OsPSTOL1 negatively impacts total root length and total root surface area of rice grown under low Pi. Interestingly, our data also showed that overexpression of OsPSTOL1 in a non-cereal species, Arabidopsis, also positively impacts overall plant growth under low Pi by modulating root development. Taken together our data provide new evidence for the role of PSTOL1 SUMOylation in mediating enhanced root development for tolerating phosphate-limiting conditions.

Citation

Mukkawar, V., Roy, D., Sue-ob, K., Jones, A., Zhang, C., Kumar Bhagat, P., …Sadanandom, A. (2024). SUMOylation of OsPSTOL1 is essential for regulating phosphate starvation responses in rice and Arabidopsis. Frontiers in Plant Science, 15, Article 1274610. https://doi.org/10.3389/fpls.2024.1274610

Journal Article Type Article
Acceptance Date Jan 31, 2024
Online Publication Date Mar 7, 2024
Publication Date Mar 7, 2024
Deposit Date May 23, 2024
Publicly Available Date Jun 12, 2024
Journal Frontiers in Plant Science
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 15
Article Number 1274610
DOI https://doi.org/10.3389/fpls.2024.1274610
Public URL https://durham-repository.worktribe.com/output/2346247
Additional Information Corrigendum: SUMOylation of OsPSTOL1 is essential for regulating phosphate starvation responses in rice and Arabidopsis: https://doi.org/10.3389/fpls.2024.1412657

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Copyright Statement
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.





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