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SUMO Suppresses the Activity of the Jasmonic Acid Receptor CORONATINE INSENSITIVE1

Srivastava, Anjil Kumar; Orosa, Beatriz; Singh, Prashant; Cummins, Ian; Walsh, Charlotte; Zhang, Cunjin; Grant, Murray; Roberts, Michael R.; Anand, Ganesh Srinivasan; Fitches, Elaine; Sadanandom, Ari

SUMO Suppresses the Activity of the Jasmonic Acid Receptor CORONATINE INSENSITIVE1 Thumbnail


Authors

Anjil Kumar Srivastava

Beatriz Orosa

Prashant Singh

Ian Cummins

Charlotte Walsh

Murray Grant

Michael R. Roberts

Ganesh Srinivasan Anand



Abstract

Plants respond rapidly to sudden environmental cues, often responding prior to changes in the hormone levels that coordinate these responses. How this is achieved is not fully understood. The integrative role of the phytohormone jasmonic acid (JA) relies upon the plant's ability to control the levels of JASMONATE ZIM (JAZ) domain-containing repressor proteins. Here, we demonstrate that regardless of intrinsic JA levels, Small Ubiquitin-like Modifier (SUMO)-conjugated JAZ proteins inhibit the JA receptor CORONATINE INSENSITIVE1 (COI1) from mediating non-SUMOylated JAZ degradation. The SUMO-deconjugating proteases OVERLY TOLERANT TO SALT1 (OTS1) and OTS2 regulate JAZ protein SUMOylation and stability. The ots1 ots2 double mutants accumulate SUMOylated and non-SUMOylated JAZ repressor proteins but show no change in endogenous JA levels compared with wild-type plants. SUMO1-conjugated JAZ proteins bind to COI1 independently of the JA mimic coronatine. SUMO inhibits JAZ binding to COI1. We identify the SUMO interacting motif in COI1 and demonstrate that this is vital to SUMO-dependent inhibition of COI1. Necrotroph infection of Arabidopsis thaliana promotes SUMO protease degradation, and this increases JAZ SUMOylation and abundance, which in turn inhibits JA signaling. This study reveals a mechanism for rapidly regulating JA responses, allowing plants to adapt to environmental changes.

Citation

Srivastava, A. K., Orosa, B., Singh, P., Cummins, I., Walsh, C., Zhang, C., …Sadanandom, A. (2018). SUMO Suppresses the Activity of the Jasmonic Acid Receptor CORONATINE INSENSITIVE1. The Plant Cell, 30(9), 2099-2115. https://doi.org/10.1105/tpc.18.00036

Journal Article Type Article
Acceptance Date Aug 10, 2018
Online Publication Date Aug 16, 2018
Publication Date Sep 1, 2018
Deposit Date Oct 18, 2018
Publicly Available Date Oct 18, 2018
Journal Plant Cell
Print ISSN 1040-4651
Electronic ISSN 1532-298X
Publisher Oxford University Press
Peer Reviewed Peer Reviewed
Volume 30
Issue 9
Pages 2099-2115
DOI https://doi.org/10.1105/tpc.18.00036

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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
© The Author(s) 2018. Published by Oxford University Press on behalf of American Society of Plant Biologists. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.







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