Bright, highly water soluble triazacyclononane europium complexes to detect ligand binding with time-resolved FRET microscopy
Delbianco, M.; Sadovnikova, V.; Bourrier, E.; Mathis, G.; Lamarque, L.; Zwier, J.M.; Parker, D.
Professor David Parker firstname.lastname@example.org
Luminescent europium complexes are used in a broad range of applications as a result of their particular emissive properties. The synthesis and application of bright, highly water-soluble, and negatively charged sulfonic- or carboxylic acid derivatives of para-substituted aryl–alkynyl triazacyclononane complexes are described. Introduction of the charged solubilizing moieties suppresses cellular uptake or adsorption to living cells making them applicable for labeling and performing assays on membrane receptors. These europium complexes are applied to monitor fluorescent ligand binding on cell-surface proteins with time-resolved Förster resonance energy transfer (TR-FRET) assays in plate-based format and using TR-FRET microscopy.
Delbianco, M., Sadovnikova, V., Bourrier, E., Mathis, G., Lamarque, L., Zwier, J., & Parker, D. (2014). Bright, highly water soluble triazacyclononane europium complexes to detect ligand binding with time-resolved FRET microscopy. Angewandte Chemie International Edition, 53(40), 10718-10722. https://doi.org/10.1002/anie.201406632
|Journal Article Type||Article|
|Online Publication Date||Sep 26, 2014|
|Publication Date||Sep 26, 2014|
|Deposit Date||Nov 14, 2014|
|Publicly Available Date||Dec 1, 2014|
|Journal||Angewandte Chemie International Edition|
|Peer Reviewed||Peer Reviewed|
|Keywords||Europium, G protein-coupled receptors, Imaging agents, Macrocycles, Time-resolved FRET.|
Accepted Journal Article
This is the peer reviewed version of the following article: Delbianco, M., Sadovnikova, V., Bourrier, E., Mathis, G., Lamarque, L., Zwier, J. M. and Parker, D. (2014), Bright, Highly Water-Soluble Triazacyclononane Europium Complexes To Detect Ligand Binding with Time-Resolved FRET Microscopy. Angewandte Chemie International Edition, 53 (40): 10718-10722, which has been published in final form at http://dx.doi.org/10.1002/anie.201406632. This article may be used for non-commercial purposes in accordance With Wiley-VCH Terms and Conditions for self-archiving.