Triggered Functional Dynamics of AsLOV2 by Time‐Resolved Electron Paramagnetic Resonance at High Magnetic Fields
Maity, Shiny; Price, Brad D.; Wilson, C. Blake; Mukherjee, Arnab; Starck, Matthieu; Parker, David; Wilson, Maxwell Z.; Lovett, Janet E.; Han, Songi; Sherwin, Mark S.
Brad D. Price
C. Blake Wilson
Professor David Parker firstname.lastname@example.org
Maxwell Z. Wilson
Janet E. Lovett
Mark S. Sherwin
We present time-resolved Gd−Gd electron paramagnetic resonance (TiGGER) at 240 GHz for tracking inter-residue distances during a protein's mechanical cycle in the solution state. TiGGER makes use of Gd-sTPATCN spin labels, whose favorable qualities include a spin-7/2 EPR-active center, short linker, narrow intrinsic linewidth, and virtually no anisotropy at high fields (8.6 T) when compared to nitroxide spin labels. Using TiGGER, we determined that upon light activation, the C-terminus and N-terminus of AsLOV2 separate in less than 1 s and relax back to equilibrium with a time constant of approximately 60 s. TiGGER revealed that the light-activated long-range mechanical motion is slowed in the Q513A variant of AsLOV2 and is correlated to the similarly slowed relaxation of the optically excited chromophore as described in recent literature. TiGGER has the potential to valuably complement existing methods for the study of triggered functional dynamics in proteins.
Maity, S., Price, B. D., Wilson, C. B., Mukherjee, A., Starck, M., Parker, D., …Sherwin, M. S. (2023). Triggered Functional Dynamics of AsLOV2 by Time‐Resolved Electron Paramagnetic Resonance at High Magnetic Fields. Angewandte Chemie International Edition, 62(13), Article e202212832. https://doi.org/10.1002/anie.202212832
|Journal Article Type||Article|
|Online Publication Date||Mar 14, 2023|
|Publication Date||Mar 20, 2023|
|Deposit Date||May 5, 2023|
|Publicly Available Date||Mar 15, 2024|
|Journal||Angewandte Chemie International Edition|
|Peer Reviewed||Peer Reviewed|
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