Single-Nanoparticle Cell Barcoding by Tunable FRET from Lanthanides to Quantum Dots

Chen, Chi; Ao, Lijiao; Wu, Yu-Tang; Cifliku, Vjona; Cardoso Dos Santos, Marcelina; Bourrier, Emmanuel; Delbianco, Martina; Parker, David; Zwier, Jurriaan; Huang, Liang; Hildebrandt, Niko

doi:10.1002/ange.201807585

Single-Nanoparticle Cell Barcoding by Tunable FRET from Lanthanides to Quantum Dots

Chen, Chi; Ao, Lijiao; Wu, Yu-Tang; Cifliku, Vjona; Cardoso Dos Santos, Marcelina; Bourrier, Emmanuel; Delbianco, Martina; Parker, David; Zwier, Jurriaan; Huang, Liang; Hildebrandt, Niko

Authors

Chi Chen

Lijiao Ao

Yu-Tang Wu

Vjona Cifliku

Marcelina Cardoso Dos Santos

Emmanuel Bourrier

Martina Delbianco

David Parker david.parker@durham.ac.uk
Emeritus Professor

Jurriaan Zwier

Liang Huang

Niko Hildebrandt

Abstract

Fluorescence barcoding based on nanoparticles provides many advantages for multiparameter imaging. However, creating different concentration‐independent codes without mixing various nanoparticles and by using single wavelength excitation and emission for multiplexed cellular imaging is extremely challenging. Here, we report the development of quantum dots (QDs) with two different SiO2 shell thicknesses (6 and 12 nm) and coated with two different lanthanide complexes (Tb and Eu). FRET from Tb or Eu donors to the QD acceptors resulted in four distinct photoluminescence (PL) decays, which were encoded by simple time‐gated (TG) PL intensity detection in three individual temporal detection windows. The well‐defined single‐nanoparticle codes were used for live cell imaging and a one‐measurement distinction of four different cells in a single field of view. This single‐color barcoding strategy opens new opportunities for multiplexed labeling and tracking of cells.

Citation

Chen, C., Ao, L., Wu, Y.-T., Cifliku, V., Cardoso Dos Santos, M., Bourrier, E., Delbianco, M., Parker, D., Zwier, J., Huang, L., & Hildebrandt, N. (2018). Single-Nanoparticle Cell Barcoding by Tunable FRET from Lanthanides to Quantum Dots. Angewandte Chemie, 130(41), 13876-13881. https://doi.org/10.1002/ange.201807585

Journal Article Type	Article
Acceptance Date	Aug 7, 2018
Online Publication Date	Aug 28, 2018
Publication Date	Oct 8, 2018
Deposit Date	Aug 8, 2018
Publicly Available Date	Aug 28, 2019
Journal	Angewandte Chemie
Print ISSN	0044-8249
Electronic ISSN	1521-3757
Publisher	Wiley
Peer Reviewed	Peer Reviewed
Volume	130
Issue	41
Pages	13876-13881
DOI	https://doi.org/10.1002/ange.201807585
Public URL	https://durham-repository.worktribe.com/output/1324351

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Copyright Statement
This is the accepted version of the following article: Chen, Chi, Ao, Lijiao, Wu, Yu-Tang, Cifliku, Vjona, Cardoso Dos Santos, Marcelina, Bourrier, Emmanuel, Delbianco, Martina, Parker, David, Zwier, Jurriaan, Huang, Liang & Hildebrandt, Niko (2018). Single-Nanoparticle Cell Barcoding by Tunable FRET from Lanthanides to Quantum Dots. Angewandte Chemie 130(41): 13876-13881 which has been published in final form at https://doi.org/10.1002/ange.201807585. This article may be used for non-commercial purposes in accordance With Wiley-VCH Terms and Conditions for self-archiving.