Skip to main content

Research Repository

Advanced Search

Bionomics and insecticide resistance of the arboviral vector Aedes albopictus in northern Lao PDR

Tangena, Julie-Anne A.; Marcombe, Sébastien; Thammavong, Phoutmany; Chonephetsarath, Somsanith; Somphong, Boudsady; Sayteng, Kouxiong; Grandadam, Marc; Sutherland, Ian W.; Lindsay, Steve W.; Brey, Paul T.

Bionomics and insecticide resistance of the arboviral vector Aedes albopictus in northern Lao PDR Thumbnail


Authors

Julie-Anne A. Tangena

Sébastien Marcombe

Phoutmany Thammavong

Somsanith Chonephetsarath

Boudsady Somphong

Kouxiong Sayteng

Marc Grandadam

Ian W. Sutherland

Paul T. Brey



Abstract

In the last four decades there has been a staggering increase in the geographical range of the arboviral vector Aedes albopictus (Skuse, 1894). This species is now found in every continent except Antarctica, increasing the distribution of arboviral diseases such as dengue and chikungunya. In Lao PDR dengue epidemics occur regularly, with cases of chikungunya also reported. As treatment methods for arboviral diseases is limited, the control of the vector mosquitoes are essential. There is a paucity of information on the bionomics and resistance status of this mosquito for successful vector control efforts. Here we describe the bionomics and insecticide resistance status of Ae. albopictus in Laos to identify opportunities for control. Adult Ae. albopictus were collected using human-baited double bed net (HDN) traps in forests, villages and rubber plantations and tested for alpha- and flaviviruses with RT-PCR. Surveys were also conducted to identify larval habitats. Seven adult and larval populations originating from Vientiane Capital and Luang Prabang province were tested against DDT, malathion, permethrin, deltamethrin and, temephos following WHO protocols. Aedes albopictus were found throughout the year, but were six-fold greater in the rainy season than the dry season. Adult females were active for 24 hours, with peak of behaviour at 18.00 h. The secondary forest and rubber plantation samples showed evidence of Pan-flaviviruses, while samples from the villages did not. More than half of the emerged Ae. albopictus were collected from mature rubber plantations (53.9%; 1,533/2,845). Most Ae. albopictus mosquitoes emerged from latex collection cups (19.7%; 562/2,845), small water containers (19.7%; 562/2,845) and tyres (17.4%; 495/2,845). Adult mosquitoes were susceptible to pyrethroids, apart from one population in Vientiane city. All populations were resistant to DDT (between 27–90% mortality) and all except one were resistant to malathion (20–86%). Three of the seven larval populations were resistant to temephos (42–87%), with suspected resistance found in three other populations (92–98%).This study demonstrates that rural areas in northern Laos are potential hot spots for arboviral disease transmission. Multiple-insecticide resistance was found. Aedes albopictus control efforts in villages need to expand to include secondary forests and rubber plantations, with larval source management and limited use of insecticides.

Citation

Tangena, J. A., Marcombe, S., Thammavong, P., Chonephetsarath, S., Somphong, B., Sayteng, K., …Brey, P. T. (2018). Bionomics and insecticide resistance of the arboviral vector Aedes albopictus in northern Lao PDR. PLoS ONE, 13(10), Article e0206387. https://doi.org/10.1371/journal.pone.0206387

Journal Article Type Article
Acceptance Date Oct 1, 2018
Online Publication Date Oct 25, 2018
Publication Date Oct 25, 2018
Deposit Date Nov 8, 2018
Publicly Available Date Nov 8, 2018
Journal PLoS ONE
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 13
Issue 10
Article Number e0206387
DOI https://doi.org/10.1371/journal.pone.0206387

Files

Published Journal Article (2.1 Mb)
PDF

Publisher Licence URL
https://creativecommons.org/licenses/publicdomain

Copyright Statement
This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.







You might also like



Downloadable Citations