Root branching toward water involves posttranslational modification of transcription factor ARF7

Orosa-Puente, Beatriz; Leftley, Nicola; von Wangenheim, Daniel; Banda, Jason; Srivastava, Anjil K.; Hill, Kristine; Truskina, Jekaterina; Bhosale, Rahul; Morris, Emily; Srivastava, Moumita; Kümpers, Britta; Goh, Tatsuaki; Fukaki, Hidehiro; Vermeer, Joop E.M.; Vernoux, Teva; Dinneny, José R.; French, Andrew P.; Bishopp, Anthony; Sadanandom, Ari; Bennett, Malcolm J.

Abstract

Plants adapt to heterogeneous soil conditions by altering their root architecture. For example, roots branch when in contact with water using the hydropatterning response. We report that hydropatterning is dependent on auxin response factor ARF7. This transcription 5 factor induces asymmetric expression of its target gene LBD16 in lateral root founder cells on the side of the root in contact with water. This differential expression pattern is regulated by post-translational modification of ARF7 with the SUMO protein. SUMOylation negatively regulates ARF7 DNA binding activity. ARF7 SUMOylation is required to recruit the Aux/IAA repressor protein IAA3. Blocking ARF7 SUMOylation disrupts IAA3 10 recruitment and hydropatterning. We conclude that SUMO-dependent regulation of auxin response controls root branching pattern in response to water availability.

Citation

Orosa-Puente, B., Leftley, N., von Wangenheim, D., Banda, J., Srivastava, A. K., Hill, K., Truskina, J., Bhosale, R., Morris, E., Srivastava, M., Kümpers, B., Goh, T., Fukaki, H., Vermeer, J. E., Vernoux, T., Dinneny, J. R., French, A. P., Bishopp, A., Sadanandom, A., & Bennett, M. J. (2018). Root branching toward water involves posttranslational modification of transcription factor ARF7. Science, 362(6421), 1407-1410. https://doi.org/10.1126/science.aau3956

• Journal Article Type: Article
• Acceptance Date: Nov 6, 2018
• Online Publication Date: Dec 21, 2018
• Publication Date: Dec 21, 2018
• Deposit Date: Feb 8, 2019
• Publicly Available Date: Mar 8, 2019
• Journal: Science
• Print ISSN: 0036-8075
• Electronic ISSN: 1095-9203
• Publisher: American Association for the Advancement of Science
• Peer Reviewed: Peer Reviewed
• Volume: 362
• Issue: 6421
• Pages: 1407-1410
• DOI: https://doi.org/10.1126/science.aau3956
• Public URL: https://durham-repository.worktribe.com/output/1303482