The chromosome-scale reference genome of black pepper (Piper nigrum L.) provides further insight into piperine biosynthesis

Hu, L.; Xu, Z.; Wang, M.; Fan, R.; Yuan, D.; Wu, B.; Wu, H.; Qin, X.; Yan, L.; Tan, L.; Sim, S.; Li, W.; Saski, C.S.; Daniell, H.; Wendel, J.F.; Lindsey, K.; Zhang, X.; Hao, C.; Jin, S.

doi:10.1038/s41467-019-12607-6

The chromosome-scale reference genome of black pepper (Piper nigrum L.) provides further insight into piperine biosynthesis

Hu, L.; Xu, Z.; Wang, M.; Fan, R.; Yuan, D.; Wu, B.; Wu, H.; Qin, X.; Yan, L.; Tan, L.; Sim, S.; Li, W.; Saski, C.S.; Daniell, H.; Wendel, J.F.; Lindsey, K.; Zhang, X.; Hao, C.; Jin, S.

Authors

L. Hu

Z. Xu

M. Wang

R. Fan

D. Yuan

B. Wu

H. Wu

X. Qin

L. Yan

L. Tan

S. Sim

W. Li

C.S. Saski

H. Daniell

J.F. Wendel

Professor Keith Lindsey keith.lindsey@durham.ac.uk
Professor

X. Zhang

C. Hao

S. Jin

Abstract

Black pepper (Piper nigrum), dubbed the ‘King of Spices’ and ‘Black Gold’, is one of the most widely used spices. Here, we present its reference genome assembly by integrating PacBio, 10x Chromium, BioNano DLS optical mapping, and Hi-C mapping technologies. The 761.2 Mb sequences (45 scaffolds with an N50 of 29.8 Mb) are assembled into 26 pseudochromosomes. A phylogenomic analysis of representative plant genomes places magnoliids as sister to the monocots-eudicots clade and indicates that black pepper has diverged from the shared Laurales-Magnoliales lineage approximately 180 million years ago. Comparative genomic analyses reveal specific gene expansions in the glycosyltransferase, cytochrome P450, shikimate hydroxycinnamoyl transferase, lysine decarboxylase, and acyltransferase gene families. Comparative transcriptomic analyses disclose berry-specific upregulated expression in representative genes in each of these gene families. These data provide an evolutionary perspective and shed light on the metabolic processes relevant to the molecular basis of species-specific piperine biosynthesis.

Citation

Hu, L., Xu, Z., Wang, M., Fan, R., Yuan, D., Wu, B., …Jin, S. (2019). The chromosome-scale reference genome of black pepper (Piper nigrum L.) provides further insight into piperine biosynthesis. Nature Communications, 10, Article 4702. https://doi.org/10.1038/s41467-019-12607-6

Journal Article Type	Article
Acceptance Date	Sep 16, 2019
Online Publication Date	Oct 16, 2019
Publication Date	Oct 16, 2019
Deposit Date	Oct 1, 2019
Publicly Available Date	Oct 30, 2019
Journal	Nature Communications
Publisher	Nature Research
Peer Reviewed	Peer Reviewed
Volume	10
Article Number	4702
DOI	https://doi.org/10.1038/s41467-019-12607-6
Public URL	https://durham-repository.worktribe.com/output/1284762

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