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The application of a heat‐inducible CRISPR/Cas12b (C2c1) genome editing system in tetraploid cotton (G.hirsutum) plants

Wang, Q.; Alariqi, M.; Wang, F.; Li, B.; Ding, X.; Rui, H.; Li, Y.; Xu, Z.; Qin, L.; Sun, Lin; Li, J.; Zou, J.; Lindsey, K.; Zhang, X.; Jin, S.

The application of a heat‐inducible CRISPR/Cas12b (C2c1) genome editing system in tetraploid cotton (G.hirsutum) plants Thumbnail


Authors

Q. Wang

M. Alariqi

F. Wang

B. Li

X. Ding

H. Rui

Y. Li

Z. Xu

L. Qin

Lin Sun

J. Li

J. Zou

X. Zhang

S. Jin



Abstract

The CRISPR/Cas9 and Cas12a (Cpf1) tools have been used on a large scale for genome editing. A new effector with a single nuclease domain, a relatively small size, low frequency off‐target effects and cleavage capability under high temperature has been recently established and designated CRISPR/Cas12b (C2c1). Cas12b has also shown temperature inducibility in mammalian systems. Therefore, this system is potentially valuable for editing the genomes of plant species, such as cotton, that are resistant to high temperatures. Using this new system, mutants of upland cotton were successfully generated following Agrobacterium ‐mediated genetic transformation under a range of temperatures. Transformants (explants infected by Agrobacterium ) exposed to 45°C for 4 days showed the highest editing efficiency. No off‐target mutation was detected by whole genome sequencing. Genome edits by AacCas12b in T0 generation were faithfully passed to the T1 generation. Taken together, CRISPR/Cas12b is therefore an efficient and precise tool for genome editing in cotton plants.

Citation

Wang, Q., Alariqi, M., Wang, F., Li, B., Ding, X., Rui, H., Li, Y., Xu, Z., Qin, L., Sun, L., Li, J., Zou, J., Lindsey, K., Zhang, X., & Jin, S. (2020). The application of a heat‐inducible CRISPR/Cas12b (C2c1) genome editing system in tetraploid cotton (G.hirsutum) plants. Plant Biotechnology Journal, 18(12), 2436-2443. https://doi.org/10.1111/pbi.13417

Journal Article Type Article
Acceptance Date Apr 7, 2020
Online Publication Date Jun 8, 2020
Publication Date 2020-12
Deposit Date May 22, 2020
Publicly Available Date Jun 11, 2020
Journal Plant Biotechnology Journal
Print ISSN 1467-7644
Electronic ISSN 1467-7652
Publisher Association of Applied Biologists
Peer Reviewed Peer Reviewed
Volume 18
Issue 12
Pages 2436-2443
DOI https://doi.org/10.1111/pbi.13417
Public URL https://durham-repository.worktribe.com/output/1270088

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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
Advance online version This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.






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