The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome editing in allotetraploid cotton (G. hirsutum) and creation of nontransgenic, gossypol‐free cotton

Li, B.; Liang, S.; Alariqi, M.; Wang, F.; Wang, G.; Wang, Q.; Xu, Z.; Yu, L.; Zafar, M.N.; Sun, L.; Si, H.; Yuan, D.; Guo, W.; Wang, Y.; Lindsey, K.; Zhang, X.; Jin, S.

Abstract

Cotton (Gossypium hirsutum) is an allotetraploid species and a typical thermophilic crop that can survive and grow well under temperatures up to 45°C. CRISPR/LbCpf1 (LbCas12a) is a temperature‐sensitive system for plant genome editing (Malzahn et al., 2019) and has been successfully applied in species such as rice, soybean, tobacco, maize and cotton (Lee et al., 2019; Li et al., 2018; Tang et al., 2017; Xu et al., 2019). However, the temperature sensitivity of LbCpf1 has not been tested in cotton yet, a high temperature‐resistant crop. In order to improve LbCpf1 efficiency and determine the optimum temperature for cotton genome editing, we investigated the effects of different temperatures on LbCpf1 activity and genome editing efficiency. Moreover, we created nontransgenic and glandless cotton plants with seeds free of gossypol, representing a valuable germplasm resource for cotton breeding.

Citation

Li, B., Liang, S., Alariqi, M., Wang, F., Wang, G., Wang, Q., Xu, Z., Yu, L., Zafar, M., Sun, L., Si, H., Yuan, D., Guo, W., Wang, Y., Lindsey, K., Zhang, X., & Jin, S. (2020). The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome editing in allotetraploid cotton (G. hirsutum) and creation of nontransgenic, gossypol‐free cotton. Plant Biotechnology Journal, 19(2), 221-223. https://doi.org/10.1111/pbi.13470

• Journal Article Type: Article
• Acceptance Date: Aug 11, 2020
• Online Publication Date: Sep 14, 2020
• Publication Date: 2020-02
• Deposit Date: Sep 1, 2020
• Publicly Available Date: Sep 16, 2020
• Journal: Plant Biotechnology Journal
• Print ISSN: 1467-7644
• Electronic ISSN: 1467-7652
• Publisher: Association of Applied Biologists
• Peer Reviewed: Peer Reviewed
• Volume: 19
• Issue: 2
• Pages: 221-223
• DOI: https://doi.org/10.1111/pbi.13470
• Public URL: https://durham-repository.worktribe.com/output/1263308