A single-nucleotide mutation in GLUTAMATE RECEPTOR-LIKE protein gene confers resistance to Fusarium wilt in Gossypium hirsutum
Liu, S.; Zhang, X.; Xiao, S.; Ma, J.; Shi, W.; Qin, T.; Xi, H.; Nie, X.; You, C.; Xu, Z.; Wang, T.; Wang, Y.; Zhang, Z.; Li, J.; Kong, J.; Aierxi, A.; Yu, Y.; Lindsey, K.; Klosterman, S.J.; Zhu, L.
Professor Keith Lindsey email@example.com
Fusarium wilt (FW) disease of cotton, caused by the fungus Fusarium oxysporum f. sp. vasinfectum (Fov), causes severe losses in cotton production worldwide. Though significant advancements have been made in development of FW‐resistant Upland cotton (Gossypium hirsutum) in resistance screening programs, the precise resistance genes and the corresponding molecular mechanisms for resistance to Fov remain unclear. Herein it is reported that Fov7, a gene unlike canonical plant disease‐resistance (R) genes, putatively encoding a GLUTAMATE RECEPTOR‐LIKE (GLR) protein, confers resistance to Fov race 7 in Upland cotton. A single nucleotide polymorphism (SNP) (C/A) in GhGLR4.8, resulting in an amino acid change (L/I), is associated with Fov resistance. A PCR‐based DNA marker (GhGLR4.8SNP(A/C)) is developed and shown to cosegregate with the Fov resistance. CRISPR/Cas9‐mediated knockout of Fov7 results in cotton lines extremely susceptible to Fov race 7 with a loss of the ability to induce calcium influx in response to total secreted proteins (SEPs) of Fov. Furthermore, coinfiltration of SEPs with GhGLR4.8A results in a hypersensitive response. This first report of a GLR‐encoding gene that functions as an R gene provides a new insight into plant–pathogen interactions and a new handle to develop cotton cultivars with resistance to Fov race 7.
Liu, S., Zhang, X., Xiao, S., Ma, J., Shi, W., Qin, T., …Zhu, L. (2021). A single-nucleotide mutation in GLUTAMATE RECEPTOR-LIKE protein gene confers resistance to Fusarium wilt in Gossypium hirsutum. Advanced Science, 8(7), Article 2020723. https://doi.org/10.1002/advs.202002723
|Journal Article Type||Article|
|Online Publication Date||Feb 19, 2021|
|Deposit Date||Feb 19, 2021|
|Publicly Available Date||Mar 30, 2021|
|Publisher||Wiley Open Access|
|Peer Reviewed||Peer Reviewed|
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Early View © 2021 The Authors. Advanced Science published by Wiley‐VCH GmbH<br /> <br /> This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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