Cotton pan-genome retrieves the lost sequences and genes during domestication and selection

Li, J.; Yuan, D.; Wang, P.; Wang, Q.; Sun, M.; Liu, Z.; Si, H.; Xu, Z.; Ma, Y.; Zhang, B.; Pei, L.; Tu, L.; Zhu, L.; Chen, L.-L.; Lindsey, K.; Zhang, X.; Jin, S.; Wang, M.

doi:10.1186/s13059-021-02351-w

Cotton pan-genome retrieves the lost sequences and genes during domestication and selection

Li, J.; Yuan, D.; Wang, P.; Wang, Q.; Sun, M.; Liu, Z.; Si, H.; Xu, Z.; Ma, Y.; Zhang, B.; Pei, L.; Tu, L.; Zhu, L.; Chen, L.-L.; Lindsey, K.; Zhang, X.; Jin, S.; Wang, M.

Authors

J. Li

D. Yuan

P. Wang

Q. Wang

M. Sun

Z. Liu

H. Si

Z. Xu

Y. Ma

B. Zhang

L. Pei

L. Tu

L. Zhu

L.-L. Chen

Professor Keith Lindsey keith.lindsey@durham.ac.uk
Professor

X. Zhang

S. Jin

M. Wang

Abstract

Background Millennia of directional human selection has reshaped the genomic architecture of cultivated cotton relative to wild counterparts, but we have limited understanding of the selective retention and fractionation of genomic components. Results We construct a comprehensive genomic variome based on 1961 cottons and identify 456 Mb and 357 Mb of sequence with domestication and improvement selection signals and 162 loci, 84 of which are novel, including 47 loci associated with 16 agronomic traits. Using pan-genome analyses, we identify 32,569 and 8851 non-reference genes lost from Gossypium hirsutum and Gossypium barbadense reference genomes respectively, of which 38.2% (39,278) and 14.2% (11,359) of genes exhibit presence/absence variation (PAV). We document the landscape of PAV selection accompanied by asymmetric gene gain and loss and identify 124 PAVs linked to favorable fiber quality and yield loci. Conclusions This variation repertoire points to genomic divergence during cotton domestication and improvement, which informs the characterization of favorable gene alleles for improved breeding practice using a pan-genome-based approach.

Citation

Li, J., Yuan, D., Wang, P., Wang, Q., Sun, M., Liu, Z., …Wang, M. (2021). Cotton pan-genome retrieves the lost sequences and genes during domestication and selection. Genome Biology, 22, Article 119. https://doi.org/10.1186/s13059-021-02351-w

Journal Article Type	Article
Acceptance Date	Apr 14, 2021
Online Publication Date	Apr 23, 2021
Publication Date	2021
Deposit Date	Apr 28, 2021
Publicly Available Date	Apr 30, 2021
Journal	Genome Biology
Print ISSN	1474-760X
Publisher	BioMed Central
Peer Reviewed	Not Peer Reviewed
Volume	22
Article Number	119
DOI	https://doi.org/10.1186/s13059-021-02351-w
Public URL	https://durham-repository.worktribe.com/output/1249004

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