Structural and functional characterization of the Redβ recombinase from bacteriophage λ (2013)
Journal Article
Matsubara, K., Malay, A., Curtis, F., Sharples, G., & Heddle, J. (2013). Structural and functional characterization of the Redβ recombinase from bacteriophage λ. PLoS ONE, 8(11), Article e78869. https://doi.org/10.1371/journal.pone.0078869

The Red system of bacteriophage λ is responsible for the genetic rearrangements that contribute to its rapid evolution and has been successfully harnessed as a research tool for genome manipulation. The key recombination component is Redβ, a ring-sha... Read More about Structural and functional characterization of the Redβ recombinase from bacteriophage λ.

Mutants of phage bIL67 RuvC with enhanced Holliday junction binding selectivity and resolution symmetry (2013)
Journal Article
Green, V., Curtis, F., Sedelnikova, S., Rafferty, J., & Sharples, G. (2013). Mutants of phage bIL67 RuvC with enhanced Holliday junction binding selectivity and resolution symmetry. Molecular Microbiology, 89(6), 1240-1258. https://doi.org/10.1111/mmi.12343

Viral and bacterial Holliday junction resolvases differ in specificity with the former typically being more promiscuous, acting on a variety of branched DNA substrates, while the latter exclusively targets Holliday junctions. We have determined the c... Read More about Mutants of phage bIL67 RuvC with enhanced Holliday junction binding selectivity and resolution symmetry.

Tripartite efflux pumps: energy is required for dissociation, but not assembly or opening of the outer membrane channel of the pump (2013)
Journal Article
Janganan, T., Bavro, V., Zhang, L., Borges-Walmsley, M., & Walmsley, A. (2013). Tripartite efflux pumps: energy is required for dissociation, but not assembly or opening of the outer membrane channel of the pump. Molecular Microbiology, 88(3), 590-602. https://doi.org/10.1111/mmi.12211

The MtrCDE multidrug pump, from Neisseria gonorrhoeae, is assembled from the inner and outer membrane proteins MtrD and MtrE, which are connected by the periplasmic membrane fusion protein MtrC. Although it is clear that MtrD delivers drugs to the ch... Read More about Tripartite efflux pumps: energy is required for dissociation, but not assembly or opening of the outer membrane channel of the pump.

Aqueous synthesis of N,S,-dialkylthiophosphoramidates: design, optimisation and application to library construction and antileishmanial testing (2013)
Journal Article
Trmčić, M., Chadbourne, F., Brear, P., Denny, P., Cobb, S., & Hodgson, D. (2013). Aqueous synthesis of N,S,-dialkylthiophosphoramidates: design, optimisation and application to library construction and antileishmanial testing. Organic and Biomolecular Chemistry, 11(16), 2660-2675. https://doi.org/10.1039/c3ob27448a

We recently reported the use of PSCl3 for the thiophosphorylation of alkylamines where the resulting N-thiophosphoramidate ions could be readily S-alkylated (Chem. Commun., 2011, 47, 6156–6158.). Herein we report the development of this methodology u... Read More about Aqueous synthesis of N,S,-dialkylthiophosphoramidates: design, optimisation and application to library construction and antileishmanial testing.

Sphingolipid synthesis and scavenging in the intracellular apicomplexan parasite, Toxoplasma gondii (2013)
Journal Article
Pratt, S., Wansadhipathi-Kannangara, N., Bruce, C., Mina, J., Shams-Eldin, H., Casas, J., …Denny, P. (2013). Sphingolipid synthesis and scavenging in the intracellular apicomplexan parasite, Toxoplasma gondii. Molecular and Biochemical Parasitology, 87(1), 43-51. https://doi.org/10.1016/j.molbiopara.2012.11.007

Sphingolipids are essential components of eukaryotic cell membranes, particularly the plasma membrane, and are involved in a diverse array of signal transduction pathways. Mammals produce sphingomyelin (SM) as the primary complex sphingolipid via the... Read More about Sphingolipid synthesis and scavenging in the intracellular apicomplexan parasite, Toxoplasma gondii.