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Exo84c-regulated degradation is involved in the normal self-incompatible response in Brassicaceae (2024)
Journal Article
Zhang, T., Wang, K., Dou, S., Gao, E., Hussey, P. J., Lin, Z., & Wang, P. (2024). Exo84c-regulated degradation is involved in the normal self-incompatible response in Brassicaceae. Cell Reports, 43(3), Article 113913. https://doi.org/10.1016/j.celrep.2024.113913

The self-incompatibility system evolves in angiosperms to promote cross-pollination by rejecting self-pollination. Here, we show the involvement of Exo84c in the SI response of both Brassica napus and Arabidopsis. The expression of Exo84c is specific... Read More about Exo84c-regulated degradation is involved in the normal self-incompatible response in Brassicaceae.

Studying Plant ER-PM Contact Site Localized Proteins Using Microscopy (2024)
Book Chapter
Li, L., Zhang, T., Hussey, P. J., & Wang, P. (2024). Studying Plant ER-PM Contact Site Localized Proteins Using Microscopy. In V. Kriechbaumer (Ed.), . Springer. https://doi.org/10.1007/978-1-0716-3710-4_3

As in most eukaryotic cells, the plant endoplasmic reticulum (ER) network is physically linked to the plasma membrane (PM), forming ER-PM contact sites (EPCS). The protein complex required for maintaining the EPCS is composed of ER integral membrane... Read More about Studying Plant ER-PM Contact Site Localized Proteins Using Microscopy.

Mitophagy in plants: Emerging regulators of mitochondrial targeting for selective autophagy (2024)
Journal Article
Duckney, P. J., Wang, P., & Hussey, P. J. (2024). Mitophagy in plants: Emerging regulators of mitochondrial targeting for selective autophagy. Journal of Microscopy, https://doi.org/10.1111/jmi.13267

The degradation and turnover of mitochondria is fundamental to Eukaryotes and is a key homeostatic mechanism for maintaining functional mitochondrial populations. Autophagy is an important pathway by which mitochondria are degraded, involving their s... Read More about Mitophagy in plants: Emerging regulators of mitochondrial targeting for selective autophagy.

NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity (2023)
Journal Article
Hawkins, T. J., Kopischke, M., Duckney, P. J., Rybak, K., Mentlak, D. A., Kroon, J. T. M., …Robatzek, S. (2023). NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity. Nature Communications, 14(1), Article 5848. https://doi.org/10.1038/s41467-023-41337-z

Members of the NETWORKED (NET) family are involved in actin-membrane interactions. Here we show that two members of the NET family, NET4A and NET4B, are essential for normal guard cell actin reorganization, which is a process critical for stomatal cl... Read More about NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity.

Exo84c interacts with VAP27 to regulate exocytotic compartment degradation and stigma senescence (2023)
Journal Article
Zhang, T., Li, Y., Li, C., Zang, J., Gao, E., Kroon, J. T., …Wang, P. (2023). Exo84c interacts with VAP27 to regulate exocytotic compartment degradation and stigma senescence. Nature Communications, 14(1), Article 4888. https://doi.org/10.1038/s41467-023-40729-5

In plants, exocyst subunit isoforms exhibit significant functional diversity in that they are involved in either protein secretion or autophagy, both of which are essential for plant development and survival. Although the molecular basis of autophagy... Read More about Exo84c interacts with VAP27 to regulate exocytotic compartment degradation and stigma senescence.

TraB, a novel plant ER-mitochondrial contact site protein functions as a mitophagy receptor in plants (2022)
Journal Article
Duckney, P., Li, C., Hussey, P. J., & Wang, P. (2023). TraB, a novel plant ER-mitochondrial contact site protein functions as a mitophagy receptor in plants. Autophagy, 19(7), 2148-2150. https://doi.org/10.1080/15548627.2022.2151190

Autophagic degradation of mitochondria (known as mitophagy) is known to occur in all eukaryotes, and is important for the turnover of damaged mitochondria and recycling of nutrients during starvation. Targeting of mitochondria for autophagic degradat... Read More about TraB, a novel plant ER-mitochondrial contact site protein functions as a mitophagy receptor in plants.

TraB family proteins are components of ER-mitochondrial contact sites and regulate ER-mitochondrial interactions and mitophagy (2022)
Journal Article
Li, C., Duckney, P., Zhang, T., Fu, Y., Li, X., Kroon, J., …Wang, P. (2022). TraB family proteins are components of ER-mitochondrial contact sites and regulate ER-mitochondrial interactions and mitophagy. Nature Communications, 13, Article 5658. https://doi.org/10.1038/s41467-022-33402-w

ER-mitochondria contact sites (EMCSs) are important for mitochondrial function. Here, we have identified a EMCS complex, comprising a family of uncharacterised mitochondrial outer membrane proteins, TRB1, TRB2 and the ER protein, VAP27-1. In Arabidop... Read More about TraB family proteins are components of ER-mitochondrial contact sites and regulate ER-mitochondrial interactions and mitophagy.

Membrane contact sites and cytoskeleton-membrane interactions in autophagy (2022)
Journal Article
Duckney, P. J., Wang, P., & Hussey, P. J. (2022). Membrane contact sites and cytoskeleton-membrane interactions in autophagy. FEBS Letters, 596(17), 2093-2103. https://doi.org/10.1002/1873-3468.14414

In Eukaryotes, organelle interactions occur at specialised contact sites between organelle membranes. Contact sites are regulated by specialised tethering proteins, which bring organelle membranes into close proximity, and facilitate functional cross... Read More about Membrane contact sites and cytoskeleton-membrane interactions in autophagy.

NETWORKED2‐Subfamily Proteins Regulate the Cortical Actin Cytoskeleton of Growing Pollen Tubes and Polarised Pollen Tube Growth (2021)
Journal Article
Duckney, P., Kroon, J. T., Dixon, M. R., Hawkins, T. J., Deeks, M. J., & Hussey, P. J. (2021). NETWORKED2‐Subfamily Proteins Regulate the Cortical Actin Cytoskeleton of Growing Pollen Tubes and Polarised Pollen Tube Growth. New Phytologist, 231(1), 152-164. https://doi.org/10.1111/nph.17391

We have recently characterised NET2A as a pollen‐specific actin‐binding protein which binds F‐actin at the plasma membrane of growing pollen tubes. However, the role of NET2 proteins in pollen development and fertilisation have yet to be elucidated.... Read More about NETWORKED2‐Subfamily Proteins Regulate the Cortical Actin Cytoskeleton of Growing Pollen Tubes and Polarised Pollen Tube Growth.

A novel plant actin-microtubule bridging complex regulates cytoskeletal and ER structure at ER-PM contact sites (2021)
Journal Article
Zang, J., Klemm, S., Pain, C., Duckney, P., Bao, Z., Stamm, G., …Wang, P. (2021). A novel plant actin-microtubule bridging complex regulates cytoskeletal and ER structure at ER-PM contact sites. Current Biology, 31(6), 1251-1260.e4. https://doi.org/10.1016/j.cub.2020.12.009

In plants, the cortical endoplasmic reticulum (ER) network is connected to the plasma membrane (PM) through the ER-PM contact sites (EPCSs), whose structures are maintained by EPCS resident proteins and the cytoskeleton.1, 2, 3, 4, 5, 6, 7 Strong co-... Read More about A novel plant actin-microtubule bridging complex regulates cytoskeletal and ER structure at ER-PM contact sites.

The Arabidopsis R-SNARE VAMP714 is essential for polarization of PIN proteins and auxin responses (2021)
Journal Article
Gu, X., Fonseka, K., Agneessens, J., Casson, S., Smertenko, A., Guo, G., …Lindsey, K. (2021). The Arabidopsis R-SNARE VAMP714 is essential for polarization of PIN proteins and auxin responses. New Phytologist, 230(2), 550-566. https://doi.org/10.1111/nph.17205

The plant hormone auxin and its directional intercellular transport play a major role in diverse aspects of plant growth and development. The establishment of auxin gradients requires the asymmetric distribution of members of the auxin efflux carrier... Read More about The Arabidopsis R-SNARE VAMP714 is essential for polarization of PIN proteins and auxin responses.

Light microscopy of the endoplasmic reticulum‐membrane contact sites in plants (2020)
Journal Article
Zang, J., Zhang, T., Hussey, P., & Wang, P. (2020). Light microscopy of the endoplasmic reticulum‐membrane contact sites in plants. Journal of Microscopy, 280(2), 134-139. https://doi.org/10.1111/jmi.12934

The existence of membrane contact sites (MCS) has been reported in different systems in the past decade, and their importance has been recognized by the cell biology community. Amongst all endomembrane structures, the endoplasmic reticulum (ER) plays... Read More about Light microscopy of the endoplasmic reticulum‐membrane contact sites in plants.

MTV proteins unveil ER- and microtubule-associated compartments in the plant vacuolar trafficking pathway (2020)
Journal Article
Delgadillo, M. O., Ruano, G., Zouhar, J., Sauer, M., Shen, J., Lazarova, A., …Rojo, E. (2020). MTV proteins unveil ER- and microtubule-associated compartments in the plant vacuolar trafficking pathway. Proceedings of the National Academy of Sciences, 117(18), 9884-9895. https://doi.org/10.1073/pnas.1919820117

The factors and mechanisms involved in vacuolar transport in plants, and in particular those directing vesicles to their target endomembrane compartment, remain largely unknown. To identify components of the vacuolar trafficking machinery, we searche... Read More about MTV proteins unveil ER- and microtubule-associated compartments in the plant vacuolar trafficking pathway.

Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery (2019)
Journal Article
Wang, P., Pleskot, R., Zang, J., Winkler, J., Wang, J., Yperman, K., …Hussey, P. J. (2019). Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery. Nature Communications, 10(1), Article 5132. https://doi.org/10.1038/s41467-019-12782-6

The Arabidopsis EH proteins (AtEH1/Pan1 and AtEH2/Pan1) are components of the endocytic TPLATE complex (TPC) which is essential for endocytosis. Both proteins are homologues of the yeast ARP2/3 complex activator, Pan1p. Here, we show that these prote... Read More about Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery.

An Immune-Responsive Cytoskeletal-Plasma Membrane Feedback Loop in Plants (2018)
Journal Article
Sassmann, S., Rodrigues, C., Milne, S. W., Nenninger, A., Allwood, E., Littlejohn, G. R., …Deeks, M. J. (2018). An Immune-Responsive Cytoskeletal-Plasma Membrane Feedback Loop in Plants. Current Biology, 28(13), 2136-2144. https://doi.org/10.1016/j.cub.2018.05.014

Cell wall appositions (CWAs) are produced reactively by the plant immune system to arrest microbial invasion through the local inversion of plant cell growth. This process requires the controlled invagination of the plasma membrane (PM) in coordinati... Read More about An Immune-Responsive Cytoskeletal-Plasma Membrane Feedback Loop in Plants.

Epidermal expression of a sterol biosynthesis gene regulates root growth by a non-cell autonomous mechanism in Arabidopsis (2018)
Journal Article
Short, E., Pullen, M., Imriz, G., Liu, D., Cope-Selby, N., Hetherington, F., …Lindsey, K. (2018). Epidermal expression of a sterol biosynthesis gene regulates root growth by a non-cell autonomous mechanism in Arabidopsis. Development, 145(10), Article dev160572. https://doi.org/10.1242/dev.160572

The epidermis is hypothesized to play a signalling role during plant development. One class of mutants showing defects in signal transduction and radial patterning are those in sterol biosynthesis. The expectation is that living cells require sterols... Read More about Epidermal expression of a sterol biosynthesis gene regulates root growth by a non-cell autonomous mechanism in Arabidopsis.

Characterization of Proteins Localized to Plant ER-PM Contact Sites (2017)
Book Chapter
Wang, P., Hawes, C., Richardson, C., & Hussey, P. J. (2018). Characterization of Proteins Localized to Plant ER-PM Contact Sites. In C. Hawes, & V. Kriechbaumer (Eds.), The Plant Endoplasmic Reticulum (23-31). Humana Press. https://doi.org/10.1007/978-1-4939-7389-7_3

Like in most eukaryotic cells, the plant endoplasmic reticulum (ER) network is physically linked to the plasma membrane (PM), forming ER-PM contact sites (EPCS). The protein complex required for maintaining the EPCS is composed of ER integral membran... Read More about Characterization of Proteins Localized to Plant ER-PM Contact Sites.

Actin–membrane interactions mediated by NETWORKED2 in Arabidopsis pollen tubes through associations with Pollen Receptor-Like Kinase 4 and 5 (2017)
Journal Article
Duckney, P., Deeks, M., Dixon, M., Kroon, J., Hawkins, T., & Hussey, P. (2017). Actin–membrane interactions mediated by NETWORKED2 in Arabidopsis pollen tubes through associations with Pollen Receptor-Like Kinase 4 and 5. New Phytologist, 216(4), 1170-1180. https://doi.org/10.1111/nph.14745

During fertilization, Pollen Receptor-Like Kinases (PRKs) control pollen tube growth through the pistil in response to extracellular signals, and regulate the actin cytoskeleton at the tube apex to drive tip growth. We investigated a novel link betwe... Read More about Actin–membrane interactions mediated by NETWORKED2 in Arabidopsis pollen tubes through associations with Pollen Receptor-Like Kinase 4 and 5.

Connecting membranes to the actin cytoskeleton. (2017)
Journal Article
Wang, P., Hawkins, T., & Hussey, P. (2017). Connecting membranes to the actin cytoskeleton. Current Opinion in Plant Biology, 40, 71-76. https://doi.org/10.1016/j.pbi.2017.07.008

In plants, the actin cytoskeleton plays a major role in organelle movement, cargo transport, maintaining cell polarity and controlling the morphogenesis of endomembrane systems. All of these events require a direct connection between membrane structu... Read More about Connecting membranes to the actin cytoskeleton..

NETWORKED 3B: a novel protein in the actin cytoskeleton-endoplasmic reticulum interaction (2017)
Journal Article
Wang, P., & Hussey, P. (2017). NETWORKED 3B: a novel protein in the actin cytoskeleton-endoplasmic reticulum interaction. Journal of Experimental Botany, 68(7), 1441-1450. https://doi.org/10.1093/jxb/erx047

In plants movement of the endoplasmic reticulum (ER) is dependent on the actin cytoskeleton. However little is known about proteins that link the ER membrane and the actin cytoskeleton. Here we identified a novel protein, NETWORKED 3B (NET3B), which... Read More about NETWORKED 3B: a novel protein in the actin cytoskeleton-endoplasmic reticulum interaction.

Microcompartmentation of cytosolic aldolase by interaction with the actin cytoskeleton in Arabidopsis (2017)
Journal Article
Garagounis, C., Kostaki, K., Hawkins, T. J., Cummins, I., Fricker, M. D., Hussey, P. J., …Sweetlove, L. J. (2017). Microcompartmentation of cytosolic aldolase by interaction with the actin cytoskeleton in Arabidopsis. Journal of Experimental Botany, 68(5), 885-898. https://doi.org/10.1093/jxb/erx015

Evidence is accumulating for molecular microcompartments formed when proteins interact in localized domains with the cytoskeleton, organelle surfaces, and intracellular membranes. To understand the potential functional significance of protein microco... Read More about Microcompartmentation of cytosolic aldolase by interaction with the actin cytoskeleton in Arabidopsis.

Plant Endoplasmic Reticulum-Plasma Membrane Contact Sites (2016)
Journal Article
Wang, P., Hawes, C., & Hussey, P. (2017). Plant Endoplasmic Reticulum-Plasma Membrane Contact Sites. Trends in Plant Science, 22(4), 289-297. https://doi.org/10.1016/j.tplants.2016.11.008

The endoplasmic reticulum (ER) acts as a superhighway with multiple sideroads that connects the different membrane compartments including the ER to the plasma membrane (PM). ER–PM contact sites (EPCSs) are a common feature in eukaryotic organisms, bu... Read More about Plant Endoplasmic Reticulum-Plasma Membrane Contact Sites.

Arabidopsis SYT1 maintains stability of cortical endoplasmic reticulum networks and VAP27-1-enriched endoplasmic reticulum–plasma membrane contact sites (2016)
Journal Article
Siao, W., Wang, P., Voigt, B., Hussey, P., & Baluska, F. (2016). Arabidopsis SYT1 maintains stability of cortical endoplasmic reticulum networks and VAP27-1-enriched endoplasmic reticulum–plasma membrane contact sites. Journal of Experimental Botany, 67(21), 6161-6171. https://doi.org/10.1093/jxb/erw381

Arabidopsis synaptotagmin 1 (SYT1) is localized on the endoplasmic reticulum–plasma membrane (ER–PM) contact sites in leaf and root cells. The ER–PM localization of Arabidopsis SYT1 resembles that of the extended synaptotagmins (E-SYTs) in animal cel... Read More about Arabidopsis SYT1 maintains stability of cortical endoplasmic reticulum networks and VAP27-1-enriched endoplasmic reticulum–plasma membrane contact sites.

Cyclin-dependent kinase activity enhances phosphatidylcholine biosynthesis in Arabidopsis by repressing phosphatidic acid phosphohydrolase activity (2016)
Journal Article
Craddock, C. P., Adams, N., Kroon, J. T., Bryant, F. M., Hussey, P. J., Kurup, S., & Eastmond, P. J. (2016). Cyclin-dependent kinase activity enhances phosphatidylcholine biosynthesis in Arabidopsis by repressing phosphatidic acid phosphohydrolase activity. The Plant Journal, https://doi.org/10.1111/tpj.13321

Coordination of endomembrane biogenesis with cell cycle progression is considered to be important in maintaining cell function during growth and development. We previously showed that disruption of PHOSPHATIDIC ACID PHOSPHOHYDROLASE (PAH) activity in... Read More about Cyclin-dependent kinase activity enhances phosphatidylcholine biosynthesis in Arabidopsis by repressing phosphatidic acid phosphohydrolase activity.

Arabidopsis NAP1 regulates the formation of autophagosomes (2016)
Journal Article
Wang, P., Richardson, C., Hawes, C., & Hussey, P. (2016). Arabidopsis NAP1 regulates the formation of autophagosomes. Current Biology, 26(15), 2060-2069. https://doi.org/10.1016/j.cub.2016.06.008

The SCAR/WAVE complex is required for ARP2/3-mediated actin nucleation, and these complexes are highly conserved in plants and animals [1 and 2]. Proteins from the SCAR/WAVE complex have been found to be membrane associated in plants [3]. Using fluor... Read More about Arabidopsis NAP1 regulates the formation of autophagosomes.

EXTRA SPINDLE POLES (Separase) controls anisotropic cell expansion in Norway spruce (Picea abies) embryos independently of its role in anaphase progression (2016)
Journal Article
Moschou, P., Savenkov, E., Minina, E., Fukada, K., Reza, S., Gutierrez-Beltran, E., …Bozhkov, P. (2016). EXTRA SPINDLE POLES (Separase) controls anisotropic cell expansion in Norway spruce (Picea abies) embryos independently of its role in anaphase progression. New Phytologist, 212(1), 232-243. https://doi.org/10.1111/nph.14012

The caspase-related protease separase (EXTRA SPINDLE POLES, ESP) plays a major role in chromatid disjunction and cell expansion in Arabidopsis thaliana. Whether the expansion phenotypes are linked to defects in cell division in Arabidopsis ESP mutant... Read More about EXTRA SPINDLE POLES (Separase) controls anisotropic cell expansion in Norway spruce (Picea abies) embryos independently of its role in anaphase progression.

Plant VAP27 proteins: domain characterization, intracellular localization and role in plant development (2016)
Journal Article
Wang, P., Richardson, C., Hawkins, T. J., Sparkes, I., Hawes, C., & Hussey, P. J. (2016). Plant VAP27 proteins: domain characterization, intracellular localization and role in plant development. New Phytologist, 210(4), 1311-1326. https://doi.org/10.1111/nph.13857

The endoplasmic reticulum (ER) is connected to the plasma membrane (PM) through the plant-specific NETWORKED protein, NET3C, and phylogenetically conserved vesicle-associated membrane protein-associated proteins (VAPs). Ten VAP homologues (VAP27-1 to... Read More about Plant VAP27 proteins: domain characterization, intracellular localization and role in plant development.

Interactions between plant endomembrane systems and the actin cytoskeleton (2015)
Journal Article
Wang, P., & Hussey, P. J. (2015). Interactions between plant endomembrane systems and the actin cytoskeleton. Frontiers in Plant Science, 6, Article 422. https://doi.org/10.3389/fpls.2015.00422

Membrane trafficking, organelle movement, and morphogenesis in plant cells are mainly controlled by the actin cytoskeleton. Not all proteins that regulate the cytoskeleton and membrane dynamics in animal systems have functional homologs in plants, es... Read More about Interactions between plant endomembrane systems and the actin cytoskeleton.

The Microtubule Plus-End Tracking Proteins SPR1 and EB1b Interact to Maintain Polar Cell Elongation and Directional Organ Growth in Arabidopsis (2014)
Journal Article
Galva, C., Kirik, V., Lindeboom, J., Kaloriti, D., Rancour, D., Hussey, P., …Sedbrook, J. (2014). The Microtubule Plus-End Tracking Proteins SPR1 and EB1b Interact to Maintain Polar Cell Elongation and Directional Organ Growth in Arabidopsis. The Plant Cell, 26(11), 4409-4425. https://doi.org/10.1105/tpc.114.131482

The microtubule plus-end tracking proteins (+TIPs) END BINDING1b (EB1b) and SPIRAL1 (SPR1) are required for normal cell expansion and organ growth. EB proteins are viewed as central regulators of +TIPs and cell polarity in animals; SPR1 homologs are... Read More about The Microtubule Plus-End Tracking Proteins SPR1 and EB1b Interact to Maintain Polar Cell Elongation and Directional Organ Growth in Arabidopsis.

Dissecting the regulation of pollen tube growth by modeling the interplay of hydrodynamics, cell wall and ion dynamics (2014)
Journal Article
Liu, J., & Hussey, P. (2014). Dissecting the regulation of pollen tube growth by modeling the interplay of hydrodynamics, cell wall and ion dynamics. Frontiers in Plant Science, 5, Article 392. https://doi.org/10.3389/fpls.2014.00392

Hydrodynamics, cell wall and ion dynamics are all important properties that regulate pollen tube growth. Currently, the two main pollen tube growth models, the cell wall model and the hydrodynamic model do not appear to be reconcilable. Here we devel... Read More about Dissecting the regulation of pollen tube growth by modeling the interplay of hydrodynamics, cell wall and ion dynamics.

The Plant Cytoskeleton, NET3C, and VAP27 Mediate the Link between the Plasma Membrane and Endoplasmic Reticulum (2014)
Journal Article
Wang, P., Hawkins, T. J., Richardson, C., Cummins, I., Deeks, M. J., Sparkes, I., …Hussey, P. J. (2014). The Plant Cytoskeleton, NET3C, and VAP27 Mediate the Link between the Plasma Membrane and Endoplasmic Reticulum. Current Biology, 24(12), 1397-1405. https://doi.org/10.1016/j.cub.2014.05.003

The cortical endoplasmic reticulum (ER) network in plants is a highly dynamic structure, and it contacts the plasma membrane (PM) at ER-PM anchor/contact sites. These sites are known to be essential for communication between the ER and PM for lipid t... Read More about The Plant Cytoskeleton, NET3C, and VAP27 Mediate the Link between the Plasma Membrane and Endoplasmic Reticulum.

The evolution of the actin binding NET superfamily (2014)
Journal Article
Hawkins, T., Deeks, M., Wang, P., & Hussey, P. (2014). The evolution of the actin binding NET superfamily. Frontiers in Plant Science, 5, Article 254. https://doi.org/10.3389/fpls.2014.00254

The Arabidopsis Networked (NET) superfamily are plant-specific actin binding proteins which specifically label different membrane compartments and identify specialized sites of interaction between actin and membranes unique to plants. There are 13 me... Read More about The evolution of the actin binding NET superfamily.

Elucidating the regulation of complex signalling systems in plant cells (2014)
Journal Article
Liu, J., Lindsey, K., & Hussey, P. J. (2014). Elucidating the regulation of complex signalling systems in plant cells. Biochemical Society Transactions, 42(1), 219-223. https://doi.org/10.1042/bst20130090

The pollen tube represents a model system for the study of tip growth, and the root provides a valuable system to study gene and signalling networks in plants. In the present article, using the two systems as examples, we discuss how to elucidate the... Read More about Elucidating the regulation of complex signalling systems in plant cells.

Prieurianin/endosidin 1 is an actin-stabilizing small molecule identified from a chemical genetic screen for circadian clock effectors in Arabidopsis thaliana. (2012)
Journal Article
Tóth, R., Gerding-Reimers, C., Deeks, M., Menninger, S., Gallegos, R., Tonaco, I., …Coupland, G. (2012). Prieurianin/endosidin 1 is an actin-stabilizing small molecule identified from a chemical genetic screen for circadian clock effectors in Arabidopsis thaliana. The Plant Journal, 71(2), 338-352. https://doi.org/10.1111/j.1365-313x.2012.04991.x

A Superfamily of Actin-Binding Proteins at the Actin-Membrane Nexus of Higher Plants. (2012)
Journal Article
Deeks, M. J., Calcutt, J. R., Ingle, E. K., Hawkins, T. J., Chapman, S., Richardson, A., …Hussey, P. J. (2012). at the Actin-Membrane Nexus of Higher Plants. Current Biology, 22(17), 1595-1600. https://doi.org/10.1016/j.cub.2012.06.041

Complex animals use a wide variety of adaptor proteins to produce specialized sites of interaction between actin and membranes. Plants do not have these protein families, yet actin-membrane interactions within plant cells are critical for the positio... Read More about A Superfamily of Actin-Binding Proteins at the Actin-Membrane Nexus of Higher Plants..

A Nucleotide Phosphatase Activity in the Nucleotide Binding Domain of an Orphan Resistance Protein from Rice (2012)
Journal Article
Fenyk, S., de San Eustaquio Campillo, A., Pohl, E., Hussey, P., & Cann, M. (2012). A Nucleotide Phosphatase Activity in the Nucleotide Binding Domain of an Orphan Resistance Protein from Rice. Journal of Biological Chemistry, 287(6), 4023-4032. https://doi.org/10.1074/jbc.m111.314450

Plant resistance proteins (R-proteins) are key components of the plant immune system activated in response to a plethora of different pathogens. R-proteins are P-loop NTPase superfamily members, and current models describe their main function as ATPa... Read More about A Nucleotide Phosphatase Activity in the Nucleotide Binding Domain of an Orphan Resistance Protein from Rice.

A Compartmental Model Analysis of Integrative and Self-Regulatory Ion Dynamics in Pollen Tube Growth (2010)
Journal Article
Liu, J., Piette, B., Deeks, M., Franklin-Tong, V., & Hussey, P. (2010). A Compartmental Model Analysis of Integrative and Self-Regulatory Ion Dynamics in Pollen Tube Growth. PLoS ONE, 5(10), Article e13157. https://doi.org/10.1371/journal.pone.0013157

Sexual reproduction in higher plants relies upon the polarised growth of pollen tubes. The growth-site at the pollen tube tip responds to signalling processes to successfully steer the tube to an ovule. Essential features of pollen tube growth are po... Read More about A Compartmental Model Analysis of Integrative and Self-Regulatory Ion Dynamics in Pollen Tube Growth.

A Thermodynamic Model of Microtubule Assembly and Disassembly (2009)
Journal Article
Piette, B., Liu, J., Peeters, K., Smertenko, A., Hawkins, T., Deeks, M., …Hussey, P. (2009). A Thermodynamic Model of Microtubule Assembly and Disassembly. PLoS ONE, 4(8), Article e6378. https://doi.org/10.1371/journal.pone.0006378

Microtubules are self-assembling polymers whose dynamics are essential for the normal function of cellular processes including chromosome separation and cytokinesis. Therefore understanding what factors effect microtubule growth is fundamental to our... Read More about A Thermodynamic Model of Microtubule Assembly and Disassembly.

Enzyme activities and subcellular localization of members of the Arabidopsis glutathione transferase superfamily (2009)
Journal Article
Dixon, D., Hawkins, T., Hussey, P., & Edwards, R. (2009). Enzyme activities and subcellular localization of members of the Arabidopsis glutathione transferase superfamily. Journal of Experimental Botany, 60(4), 1207-1218. https://doi.org/10.1093/jxb/ern365

Enzyme screens with Strep-tagged recombinant proteins and expression studies with the respective green fluorescent protein (GFP) fusions have been employed to examine the functional activities and subcellular localization of members of the Arabidopsi... Read More about Enzyme activities and subcellular localization of members of the Arabidopsis glutathione transferase superfamily.

Actin dynamics and the elasticity of cytoskeletal networks (2009)
Journal Article
Buxton, G., Clarke, N., & Hussey, P. (2009). Actin dynamics and the elasticity of cytoskeletal networks. Express polymer letters, 3(9), 579-587. https://doi.org/10.3144/expresspolymlett.2009.72

The structural integrity of a cell depends on its cytoskeleton, which includes an actin network. This network is transient and depends upon the continual polymerization and depolymerization of actin. The degradation of an actin network, and a corresp... Read More about Actin dynamics and the elasticity of cytoskeletal networks.

The POLARIS peptide of Arabidopsis regulates auxin transport and root growth via effects on ethylene signaling (2006)
Journal Article
Chilley, P., Casson, S., Tarkowski, P., Wang, K., Hawkins, N., Hussey, P., …Lindsey, K. (2006). The POLARIS peptide of Arabidopsis regulates auxin transport and root growth via effects on ethylene signaling. The Plant Cell, 18(11), 3058-3072. https://doi.org/10.1105/tpc.106.040790

The rate and plane of cell division and anisotropic cell growth are critical for plant development and are regulated by diverse mechanisms involving several hormone signaling pathways. Little is known about peptide signaling in plant growth; however,... Read More about The POLARIS peptide of Arabidopsis regulates auxin transport and root growth via effects on ethylene signaling.

Arabidopsis group Ie formins localize to specific cell membrane domains, interact with actin-binding proteins and cause defects in cell expansion upon aberrant expression (2005)
Journal Article
Deeks, M., Cvrckova, F., Machesky, L., Mikitova, V., Ketelaar, T., Zarsky, V., …Hussey, P. (2005). Arabidopsis group Ie formins localize to specific cell membrane domains, interact with actin-binding proteins and cause defects in cell expansion upon aberrant expression. New Phytologist, 168(3), 529-540

Arabidopsis NAP1 is essential for Arp2/3-dependent trichome morphogenesis (2004)
Journal Article
Deeks, M., Kaloriti, D., Davies, B., Malho, R., & Hussey, P. (2004). Arabidopsis NAP1 is essential for Arp2/3-dependent trichome morphogenesis. Current Biology, 14(15), 1410-1414. https://doi.org/10.1016/j.cub.2004.06.065

The dynamic nature of the eukaryotic actin cytoskeleton is essential for the locomotion of animal cells and the morphogenesis of plant and fungal cells. The F-actin nucleating/branching activity of the Arp2/3 complex is a key function for all of thes... Read More about Arabidopsis NAP1 is essential for Arp2/3-dependent trichome morphogenesis.

The actin-interacting protein AIP1 is essential for actin organization and plant development (2004)
Journal Article
Ketelaar, T., Allwood, E., Anthony, R., Voigt, B., Menzel, D., & Hussey, P. (2004). The actin-interacting protein AIP1 is essential for actin organization and plant development. Current Biology, 14(2), 145-149. https://doi.org/10.1016/j.cub.2004.01.004

Cell division, growth, and cytoplasmic organization require a dynamic actin cytoskeleton. The filamentous actin (F-actin) network is regulated by actin binding proteins that modulate actin dynamics. These actin binding proteins often have cooperative... Read More about The actin-interacting protein AIP1 is essential for actin organization and plant development.

Regulation of the pollen-specific actin-depolymerizing factor LIADF1 (2002)
Journal Article
Allwood, E., Anthony, R., Smertenko, A., Reichelt, S., Drobak, B., Doonan, J., …Hussey, P. (2002). Regulation of the pollen-specific actin-depolymerizing factor LIADF1. The Plant Cell, 14(11), 2915-2927. https://doi.org/10.1105/tpc.005363

Pollen tube growth is dependent on a dynamic actin cytoskeleton, suggesting that actin-regulating proteins are involved. We have examined the regulation of the lily pollen-specific actin-depolymerizing factor (ADF) LlADF1. Its actin binding and depol... Read More about Regulation of the pollen-specific actin-depolymerizing factor LIADF1.

MOR1/GEM1 has an essential role in the plant-specific cytokinetic phragmoplast (2002)
Journal Article
Twell, D., Park, S., Hawkins, T., Schubert, D., Schmidt, R., Smertenko, A., & Hussey, P. (2002). MOR1/GEM1 has an essential role in the plant-specific cytokinetic phragmoplast. Nature Cell Biology, 4(9), 711-714. https://doi.org/10.1038/ncb844

MOR1 is a member of the MAP215 family of microtubule-associated proteins and is required to establish interphase arrays of cortical microtubules in plant cells1. Here we show that MOR1 binds microtubules in vivo, localizing to both cortical microtubu... Read More about MOR1/GEM1 has an essential role in the plant-specific cytokinetic phragmoplast.