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Professor Patrick Hussey's Outputs (107)

Plant NETWORKED and VAP27 Proteins Work in Complexes to Regulate Membrane-Based Functions (2025)
Journal Article
Duckney, P. J., Wang, P., & Hussey, P. J. (2025). Plant NETWORKED and VAP27 Proteins Work in Complexes to Regulate Membrane-Based Functions. Contact, 8, https://doi.org/10.1177/25152564251342533

Eukaryotic cells are subdivided into specialised organelle compartments, each with unique physiological environments and functions. Interaction and cross-talk between organelles is inherent to Eukaryotic life, and each organelle is physically interco... Read More about Plant NETWORKED and VAP27 Proteins Work in Complexes to Regulate Membrane-Based Functions.

Autophagy plays a dual role in chromoplast transition and degradation and is essential for fruit coloration and ripening (2025)
Journal Article
Guo, Y., Bao, Z., Shi, M., Zheng, Q., Huo, Y., Hu, R., Guan, Y., Cao, S., Hussey, P. J., Deng, X., Cheng, Y., & Wang, P. (online). Autophagy plays a dual role in chromoplast transition and degradation and is essential for fruit coloration and ripening. Autophagy, https://doi.org/10.1080/15548627.2025.2509330

The color of tomato fruits is determined by carotenoids. The process involves removing chloroplast-related components and the biogenesis of chromoplast membranes where carotenoids are stored, but how these events are coordinated is unknown. Here, we... Read More about Autophagy plays a dual role in chromoplast transition and degradation and is essential for fruit coloration and ripening.

The ER-PM interaction is essential for cytokinesis and recruits the actin cytoskeleton through the SCAR/WAVE complex (2025)
Journal Article
Xu, Z., Zang, J., Zhang, X., Zheng, Q., Li, Y., Field, N., Fiserova, J., Hua, B., Qu, X., Kriechbaumer, V., Deeks, M. J., Hussey, P. J., & Wang, P. (2025). The ER-PM interaction is essential for cytokinesis and recruits the actin cytoskeleton through the SCAR/WAVE complex. Proceedings of the National Academy of Sciences, 122(6), Article e2416927122. https://doi.org/10.1073/pnas.2416927122

Plant cytokinesis requires coordination between the actin cytoskeleton, microtubules 32 and membranes to guide division plane formation and cell plate expansion; how these 33 regulatory factors are coordinated remains unknown. The actin cytoskeleton... Read More about The ER-PM interaction is essential for cytokinesis and recruits the actin cytoskeleton through the SCAR/WAVE complex.

Exo84c-regulated degradation is involved in the normal self-incompatible response in Brassicaceae (2024)
Journal Article
Zhang, T., Wang, K., Dou, S., Gao, E., Hussey, P. J., Lin, Z., & Wang, P. (2024). Exo84c-regulated degradation is involved in the normal self-incompatible response in Brassicaceae. Cell Reports, 43(3), Article 113913. https://doi.org/10.1016/j.celrep.2024.113913

The self-incompatibility system evolves in angiosperms to promote cross-pollination by rejecting self-pollination. Here, we show the involvement of Exo84c in the SI response of both Brassica napus and Arabidopsis. The expression of Exo84c is specific... Read More about Exo84c-regulated degradation is involved in the normal self-incompatible response in Brassicaceae.

Studying Plant ER-PM Contact Site Localized Proteins Using Microscopy (2024)
Book Chapter
Li, L., Zhang, T., Hussey, P. J., & Wang, P. (2024). Studying Plant ER-PM Contact Site Localized Proteins Using Microscopy. In V. Kriechbaumer (Ed.), . Springer. https://doi.org/10.1007/978-1-0716-3710-4_3

As in most eukaryotic cells, the plant endoplasmic reticulum (ER) network is physically linked to the plasma membrane (PM), forming ER-PM contact sites (EPCS). The protein complex required for maintaining the EPCS is composed of ER integral membrane... Read More about Studying Plant ER-PM Contact Site Localized Proteins Using Microscopy.

Mitophagy in plants: Emerging regulators of mitochondrial targeting for selective autophagy (2024)
Journal Article
Duckney, P. J., Wang, P., & Hussey, P. J. (2025). Mitophagy in plants: Emerging regulators of mitochondrial targeting for selective autophagy. Journal of Microscopy, 297(3), 325-332. https://doi.org/10.1111/jmi.13267

The degradation and turnover of mitochondria is fundamental to Eukaryotes and is a key homeostatic mechanism for maintaining functional mitochondrial populations. Autophagy is an important pathway by which mitochondria are degraded, involving their s... Read More about Mitophagy in plants: Emerging regulators of mitochondrial targeting for selective autophagy.

NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity (2023)
Journal Article
Hawkins, T. J., Kopischke, M., Duckney, P. J., Rybak, K., Mentlak, D. A., Kroon, J. T. M., …Robatzek, S. (2023). NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity. Nature Communications, 14(1), Article 5848. https://doi.org/10.1038/s41467-023-41337-z

Members of the NETWORKED (NET) family are involved in actin-membrane interactions. Here we show that two members of the NET family, NET4A and NET4B, are essential for normal guard cell actin reorganization, which is a process critical for stomatal cl... Read More about NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity.

Exo84c interacts with VAP27 to regulate exocytotic compartment degradation and stigma senescence (2023)
Journal Article
Zhang, T., Li, Y., Li, C., Zang, J., Gao, E., Kroon, J. T., …Wang, P. (2023). Exo84c interacts with VAP27 to regulate exocytotic compartment degradation and stigma senescence. Nature Communications, 14(1), Article 4888. https://doi.org/10.1038/s41467-023-40729-5

In plants, exocyst subunit isoforms exhibit significant functional diversity in that they are involved in either protein secretion or autophagy, both of which are essential for plant development and survival. Although the molecular basis of autophagy... Read More about Exo84c interacts with VAP27 to regulate exocytotic compartment degradation and stigma senescence.

Keep in contact: multiple roles of endoplasmic reticulum–membrane contact sites and the organelle interaction network in plants (2023)
Journal Article
Wang, P., Duckney, P., Gao, E., Hussey, P. J., Kriechbaumer, V., Li, C., Zang, J., & Zhang, T. (2023). Keep in contact: multiple roles of endoplasmic reticulum–membrane contact sites and the organelle interaction network in plants. New Phytologist, 238(2), 482-499. https://doi.org/10.1111/nph.18745

Functional regulation and structural maintenance of the different organelles in plants contribute directly to plant development, reproduction and stress responses. To ensure these activities take place effectively, cells have evolved an interconnecte... Read More about Keep in contact: multiple roles of endoplasmic reticulum–membrane contact sites and the organelle interaction network in plants.

TraB, a novel plant ER-mitochondrial contact site protein functions as a mitophagy receptor in plants (2022)
Journal Article
Duckney, P., Li, C., Hussey, P. J., & Wang, P. (2023). TraB, a novel plant ER-mitochondrial contact site protein functions as a mitophagy receptor in plants. Autophagy, 19(7), 2148-2150. https://doi.org/10.1080/15548627.2022.2151190

Autophagic degradation of mitochondria (known as mitophagy) is known to occur in all eukaryotes, and is important for the turnover of damaged mitochondria and recycling of nutrients during starvation. Targeting of mitochondria for autophagic degradat... Read More about TraB, a novel plant ER-mitochondrial contact site protein functions as a mitophagy receptor in plants.

TraB family proteins are components of ER-mitochondrial contact sites and regulate ER-mitochondrial interactions and mitophagy (2022)
Journal Article
Li, C., Duckney, P., Zhang, T., Fu, Y., Li, X., Kroon, J., De Jaeger, G., Cheng, Y., Hussey, P. J., & Wang, P. (2022). TraB family proteins are components of ER-mitochondrial contact sites and regulate ER-mitochondrial interactions and mitophagy. Nature Communications, 13, Article 5658. https://doi.org/10.1038/s41467-022-33402-w

ER-mitochondria contact sites (EMCSs) are important for mitochondrial function. Here, we have identified a EMCS complex, comprising a family of uncharacterised mitochondrial outer membrane proteins, TRB1, TRB2 and the ER protein, VAP27-1. In Arabidop... Read More about TraB family proteins are components of ER-mitochondrial contact sites and regulate ER-mitochondrial interactions and mitophagy.

Membrane contact sites and cytoskeleton-membrane interactions in autophagy (2022)
Journal Article
Duckney, P. J., Wang, P., & Hussey, P. J. (2022). Membrane contact sites and cytoskeleton-membrane interactions in autophagy. FEBS Letters, 596(17), 2093-2103. https://doi.org/10.1002/1873-3468.14414

In Eukaryotes, organelle interactions occur at specialised contact sites between organelle membranes. Contact sites are regulated by specialised tethering proteins, which bring organelle membranes into close proximity, and facilitate functional cross... Read More about Membrane contact sites and cytoskeleton-membrane interactions in autophagy.

NETWORKED2‐Subfamily Proteins Regulate the Cortical Actin Cytoskeleton of Growing Pollen Tubes and Polarised Pollen Tube Growth (2021)
Journal Article
Duckney, P., Kroon, J. T., Dixon, M. R., Hawkins, T. J., Deeks, M. J., & Hussey, P. J. (2021). NETWORKED2‐Subfamily Proteins Regulate the Cortical Actin Cytoskeleton of Growing Pollen Tubes and Polarised Pollen Tube Growth. New Phytologist, 231(1), 152-164. https://doi.org/10.1111/nph.17391

We have recently characterised NET2A as a pollen‐specific actin‐binding protein which binds F‐actin at the plasma membrane of growing pollen tubes. However, the role of NET2 proteins in pollen development and fertilisation have yet to be elucidated.... Read More about NETWORKED2‐Subfamily Proteins Regulate the Cortical Actin Cytoskeleton of Growing Pollen Tubes and Polarised Pollen Tube Growth.

A novel plant actin-microtubule bridging complex regulates cytoskeletal and ER structure at ER-PM contact sites (2021)
Journal Article
Zang, J., Klemm, S., Pain, C., Duckney, P., Bao, Z., Stamm, G., Kriechbaumer, V., Bürstenbinder, K., Hussey, P. J., & Wang, P. (2021). A novel plant actin-microtubule bridging complex regulates cytoskeletal and ER structure at ER-PM contact sites. Current Biology, 31(6), 1251-1260.e4. https://doi.org/10.1016/j.cub.2020.12.009

In plants, the cortical endoplasmic reticulum (ER) network is connected to the plasma membrane (PM) through the ER-PM contact sites (EPCSs), whose structures are maintained by EPCS resident proteins and the cytoskeleton.1, 2, 3, 4, 5, 6, 7 Strong co-... Read More about A novel plant actin-microtubule bridging complex regulates cytoskeletal and ER structure at ER-PM contact sites.

The Arabidopsis R-SNARE VAMP714 is essential for polarization of PIN proteins and auxin responses (2021)
Journal Article
Gu, X., Fonseka, K., Agneessens, J., Casson, S., Smertenko, A., Guo, G., Topping, J., Hussey, P., & Lindsey, K. (2021). The Arabidopsis R-SNARE VAMP714 is essential for polarization of PIN proteins and auxin responses. New Phytologist, 230(2), 550-566. https://doi.org/10.1111/nph.17205

The plant hormone auxin and its directional intercellular transport play a major role in diverse aspects of plant growth and development. The establishment of auxin gradients requires the asymmetric distribution of members of the auxin efflux carrier... Read More about The Arabidopsis R-SNARE VAMP714 is essential for polarization of PIN proteins and auxin responses.

Light microscopy of the endoplasmic reticulum‐membrane contact sites in plants (2020)
Journal Article
Zang, J., Zhang, T., Hussey, P., & Wang, P. (2020). Light microscopy of the endoplasmic reticulum‐membrane contact sites in plants. Journal of Microscopy, 280(2), 134-139. https://doi.org/10.1111/jmi.12934

The existence of membrane contact sites (MCS) has been reported in different systems in the past decade, and their importance has been recognized by the cell biology community. Amongst all endomembrane structures, the endoplasmic reticulum (ER) plays... Read More about Light microscopy of the endoplasmic reticulum‐membrane contact sites in plants.

MTV proteins unveil ER- and microtubule-associated compartments in the plant vacuolar trafficking pathway (2020)
Journal Article
Delgadillo, M. O., Ruano, G., Zouhar, J., Sauer, M., Shen, J., Lazarova, A., Sanmartín, M., Lai, L. T. F., Deng, C., Wang, P., Hussey, P. J., Sánchez-Serrano, J. J., Jiang, L., & Rojo, E. (2020). MTV proteins unveil ER- and microtubule-associated compartments in the plant vacuolar trafficking pathway. Proceedings of the National Academy of Sciences, 117(18), 9884-9895. https://doi.org/10.1073/pnas.1919820117

The factors and mechanisms involved in vacuolar transport in plants, and in particular those directing vesicles to their target endomembrane compartment, remain largely unknown. To identify components of the vacuolar trafficking machinery, we searche... Read More about MTV proteins unveil ER- and microtubule-associated compartments in the plant vacuolar trafficking pathway.