Dr Gary Sharples' Outputs (5)

Resolution of Holliday junctions in Escherichia coli: identification of the ruvC gene product as a 19-kilodalton protein. (1991)
Journal Article
Sharples, G., & Lloyd, R. (1991). Resolution of Holliday junctions in Escherichia coli: identification of the ruvC gene product as a 19-kilodalton protein. Journal of Bacteriology, 173(23), 7711-7715

The ruvC gene of Escherichia coli specifies a nuclease that resolves Holliday junction intermediates in genetic recombination (B. Connolly, C.A. Parsons, F.E. Benson, H.J. Dunderdale, G.J. Sharples, R.G. Lloyd, and S.C. West, Proc. Natl. Acad, Sci. U... Read More about Resolution of Holliday junctions in Escherichia coli: identification of the ruvC gene product as a 19-kilodalton protein..

Formation and resolution of recombination intermediates by E. coli RecA and RuvC proteins. (1991)
Journal Article
Dunderdale, H., Benson, F., Parsons, C., Sharples, G., Lloyd, R., & West, S. (1991). Formation and resolution of recombination intermediates by E. coli RecA and RuvC proteins. Nature, 354(6354), 506-510

The recombination of DNA molecules has been reconstituted in vitro using two purified enzymes from Escherichia coli. RecA protein catalyses homologous pairing and strand exchange reactions to form intermediate DNA structures that are acted upon by Ru... Read More about Formation and resolution of recombination intermediates by E. coli RecA and RuvC proteins..

Location of a mutation in the aspartyl-tRNA synthetase gene of Escherichia coli K12. (1991)
Journal Article
Sharples, G., & Lloyd, R. (1991). Location of a mutation in the aspartyl-tRNA synthetase gene of Escherichia coli K12. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 264(3), 93-96

A mutation (tls-1) that confers a temperature-sensitive growth phenotype in Escherichia coli was shown by DNA cloning and sequencing to be an allele of aspS, the gene for aspartyl-tRNA synthetase. The mutation, which lies near minute 41 on the geneti... Read More about Location of a mutation in the aspartyl-tRNA synthetase gene of Escherichia coli K12..

Resolution of Holliday junctions in vitro requires the Escherichia coli ruvC gene product. (1991)
Journal Article
Connolly, B., Parsons, C., Benson, F., Dunderdale, H., Sharples, G., Lloyd, R., & West, S. (1991). Resolution of Holliday junctions in vitro requires the Escherichia coli ruvC gene product. Proceedings of the National Academy of Sciences, 88(14), 6063-6067

In previous studies, Holliday junctions generated during RecA-mediated strand-exchange reactions were resolved by fractionated Escherichia coli extracts. We now report the specific binding and cleavage of synthetic Holliday junctions (50 base pairs l... Read More about Resolution of Holliday junctions in vitro requires the Escherichia coli ruvC gene product..

Molecular organization and nucleotide sequence of the recG locus of Escherichia coli K-12. (1991)
Journal Article
Lloyd, R., & Sharples, G. (1991). Molecular organization and nucleotide sequence of the recG locus of Escherichia coli K-12. Journal of Bacteriology, 173(21), 6837-6843

The nucleotide sequence of the Escherichia coli K-12 recG gene was determined. recG was identified as an open reading frame located between the spoT operon and the convergent gltS gene. It encodes a polypeptide of 693 amino acids which was identified... Read More about Molecular organization and nucleotide sequence of the recG locus of Escherichia coli K-12..