Location of a mutation in the aspartyl-tRNA synthetase gene of Escherichia coli K12.

Sharples, G.J.; Lloyd, R.G.

Abstract

A mutation (tls-1) that confers a temperature-sensitive growth phenotype in Escherichia coli was shown by DNA cloning and sequencing to be an allele of aspS, the gene for aspartyl-tRNA synthetase. The mutation, which lies near minute 41 on the genetic map, was located some 2.3 kb from the 5' end of the ruvAB operon. A DNA fragment encoding the carboxy-terminus of AspRS was found to be sufficient to allow growth of a tls-1 strain at the non-permissive temperature.

Citation

Sharples, G., & Lloyd, R. (1991). Location of a mutation in the aspartyl-tRNA synthetase gene of Escherichia coli K12. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 264(3), 93-96

• Journal Article Type: Article
• Publication Date: 1991
• Journal: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
• Print ISSN: 0027-5107
• Publisher: Elsevier
• Volume: 264
• Issue: 3
• Pages: 93-96
• Public URL: https://durham-repository.worktribe.com/output/1583648
• Publisher URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=1944398