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All Outputs (142)

Bioengineering the Human Intestinal Mucosa and the Importance of Stromal Support for Pharmacological Evaluation In Vitro (2024)
Journal Article
Freer, M., Cooper, J., Goncalves, K., & Przyborski, S. (2024). Bioengineering the Human Intestinal Mucosa and the Importance of Stromal Support for Pharmacological Evaluation In Vitro. Cells, 13(22), Article 1859. https://doi.org/10.3390/cells13221859

Drug discovery is associated with high levels of compound elimination in all stages of development. The current practices for the pharmacokinetic testing of intestinal absorption combine Transwell® inserts with the Caco-2 cell line and are associated... Read More about Bioengineering the Human Intestinal Mucosa and the Importance of Stromal Support for Pharmacological Evaluation In Vitro.

Investigation into the significant role of dermal‐epidermal interactions in skin ageing utilising a bioengineered skin construct (2024)
Journal Article
Costello, L., Goncalves, K., De Los Santos Gomez, P., Hulette, B., Dicolandrea, T., Flagler, M. J., Isfort, R., Oblong, J., Bascom, C., & Przyborski, S. (online). Investigation into the significant role of dermal‐epidermal interactions in skin ageing utilising a bioengineered skin construct. Journal of Cellular Physiology, https://doi.org/10.1002/jcp.31463

Increased prevalence of skin ageing is a growing concern due to an ageing global population and has both sociological and psychological implications. The use of more clinically predictive in vitro methods for dermatological research is becoming comm... Read More about Investigation into the significant role of dermal‐epidermal interactions in skin ageing utilising a bioengineered skin construct.

Evaluating Strategies to Assess the Differentiation Potential of Human Pluripotent Stem Cells: A Review, Analysis and Call for Innovation (2024)
Journal Article
Smith, L., Quelch-Cliffe, R., Liu, F., Aguilar, A. H., & Przyborski, S. (online). Evaluating Strategies to Assess the Differentiation Potential of Human Pluripotent Stem Cells: A Review, Analysis and Call for Innovation. Stem Cell Reviews and Reports, https://doi.org/10.1007/s12015-024-10793-5

Pluripotent stem cells have the ability to differentiate into all cells and tissues within the human body, and as a result they are attractive resources for use in basic research, drug discovery and regenerative medicine. In order to successfully ach... Read More about Evaluating Strategies to Assess the Differentiation Potential of Human Pluripotent Stem Cells: A Review, Analysis and Call for Innovation.

Modulation of the Nogo signaling pathway to overcome amyloid-β-mediated neurite inhibition in human pluripotent stem cell-derived neurites. (2024)
Journal Article
Goncalves, K., & Przyborski, S. (online). Modulation of the Nogo signaling pathway to overcome amyloid-β-mediated neurite inhibition in human pluripotent stem cell-derived neurites. Neural Regeneration Research, https://doi.org/10.4103/NRR.NRR-D-23-01628

Neuronal cell death and the loss of connectivity are two of the primary pathological mechanisms underlying Alzheimer's disease. The accumulation of amyloid-β peptides, a key hallmark of Alzheimer's disease, is believed to induce neuritic abnormalitie... Read More about Modulation of the Nogo signaling pathway to overcome amyloid-β-mediated neurite inhibition in human pluripotent stem cell-derived neurites..

Suppressed basal mitophagy drives cellular aging phenotypes that can be reversed by a p62-targeting small molecule. (2024)
Journal Article
Kelly, G., Kataura, T., Panek, J., Ma, G., Salmonowicz, H., Davis, A., Kendall, H., Brookes, C., Ayine-Tora, D. M., Banks, P., Nelson, G., Dobby, L., Pitrez, P. R., Booth, L., Costello, L., Richardson, G. D., Lovat, P., Przyborski, S., Ferreira, L., Greaves, L., …Korolchuk, V. I. (2024). Suppressed basal mitophagy drives cellular aging phenotypes that can be reversed by a p62-targeting small molecule. Developmental Cell, 59(15), 1924-1939. https://doi.org/10.1016/j.devcel.2024.04.020

Selective degradation of damaged mitochondria by autophagy (mitophagy) is proposed to play an important role in cellular homeostasis. However, the molecular mechanisms and the requirement of mitochondrial quality control by mitophagy for cellular phy... Read More about Suppressed basal mitophagy drives cellular aging phenotypes that can be reversed by a p62-targeting small molecule..

Current understanding of the interplay between extracellular matrix remodelling and gut permeability in health and disease (2024)
Journal Article
Vilardi, A., Przyborski, S., Mobbs, C., Rufini, A., & Tufarelli, C. (2024). Current understanding of the interplay between extracellular matrix remodelling and gut permeability in health and disease. Cell Death Discovery, 10(1), Article 258. https://doi.org/10.1038/s41420-024-02015-1

The intestinal wall represents an interactive network regulated by the intestinal epithelium, extracellular matrix (ECM) and mesenchymal compartment. Under healthy physiological conditions, the epithelium undergoes constant renewal and forms an integ... Read More about Current understanding of the interplay between extracellular matrix remodelling and gut permeability in health and disease.

Comparison of photodamage in non-pigmented and pigmented human skin equivalents exposed to repeated ultraviolet radiation to investigate the role of melanocytes in skin photoprotection (2024)
Journal Article
De Los Santos Gomez, P., Costello, L., Goncalves, K., & Przyborski, S. (2024). Comparison of photodamage in non-pigmented and pigmented human skin equivalents exposed to repeated ultraviolet radiation to investigate the role of melanocytes in skin photoprotection. Frontiers in Medicine, 11, Article 1355799. https://doi.org/10.3389/fmed.2024.1355799

Introduction: Daily solar ultraviolet (UV) radiation has an important impact on skin health. Understanding the initial events of the UV-induced response is critical to prevent deleterious conditions. However, studies in human volunteers have ethical,... Read More about Comparison of photodamage in non-pigmented and pigmented human skin equivalents exposed to repeated ultraviolet radiation to investigate the role of melanocytes in skin photoprotection.

Cell Senescence-Independent Changes of Human Skin Fibroblasts with Age (2024)
Journal Article
Fullard, N., Wordsworth, J., Welsh, C., Maltman, V., Bascom, C., Tasseff, R., …Shanley, D. (2024). Cell Senescence-Independent Changes of Human Skin Fibroblasts with Age. Cells, 13(8), Article 659. https://doi.org/10.3390/cells13080659

Skin ageing is defined, in part, by collagen depletion and fragmentation that leads to a loss of mechanical tension. This is currently believed to reflect, in part, the accumulation of senescent cells. We compared the expression of genes and proteins... Read More about Cell Senescence-Independent Changes of Human Skin Fibroblasts with Age.

Bioprinted autologous human skin equivalents for in vitro testing of therapeutic antibodies (2024)
Journal Article
Ahmed, M., Hill, D., Ahmed, S., Przyborski, S., Dalgarno, K., & Dickinson, A. (2024). Bioprinted autologous human skin equivalents for in vitro testing of therapeutic antibodies. International Journal of Bioprinting, 10(2), Article 1851. https://doi.org/10.36922/ijb.1851

In recent years, advances in tissue engineering have brought forward the accessibility of human skin equivalents for in vitro applications; however, the availability of human-based engineered tissue models suitable for high-throughput screening of bi... Read More about Bioprinted autologous human skin equivalents for in vitro testing of therapeutic antibodies.

An in vitro model to study immune activation, epithelial disruption and stromal remodelling in inflammatory bowel disease and fistulising Crohn’s disease (2024)
Journal Article
Mobbs, C. L., Darling, N. J., & Przyborski, S. (2024). An in vitro model to study immune activation, epithelial disruption and stromal remodelling in inflammatory bowel disease and fistulising Crohn’s disease. Frontiers in Immunology, 15, Article 1357690. https://doi.org/10.3389/fimmu.2024.1357690

At present, preclinical models of inflammatory bowel disease (IBD) are insufficient, limiting translation between research and new therapeutics. This is especially true for fistulising Crohn’s disease (CD), as the severe lack of relevant models hinde... Read More about An in vitro model to study immune activation, epithelial disruption and stromal remodelling in inflammatory bowel disease and fistulising Crohn’s disease.

Development of a novel in vitro strategy to understand the impact of shaving on skin health: combining tape strip exfoliation and human skin equivalent technology (2023)
Journal Article
Costello, L., Goncalves, K., Maltman, V., Barrett, N., Shah, K., Stephens, A., …Przyborski, S. (2023). Development of a novel in vitro strategy to understand the impact of shaving on skin health: combining tape strip exfoliation and human skin equivalent technology. Frontiers in Medicine, 10, Article 1236790. https://doi.org/10.3389/fmed.2023.1236790

Introduction: The removal of unwanted hair is a widespread grooming practice adopted by both males and females. Although many depilatory techniques are now available, shaving remains the most common, despite its propensity to irritate skin. Current t... Read More about Development of a novel in vitro strategy to understand the impact of shaving on skin health: combining tape strip exfoliation and human skin equivalent technology.

Impact of the Physical Cellular Microenvironment on the Structure and Function of a Model Hepatocyte Cell Line for Drug Toxicity Applications (2023)
Journal Article
Allcock, B., Wei, W., Goncalves, K., Hoyle, H., Robert, A., Quelch-Cliffe, R., …Przyborski, S. (2023). Impact of the Physical Cellular Microenvironment on the Structure and Function of a Model Hepatocyte Cell Line for Drug Toxicity Applications. Cells, 12(19), Article 2408. https://doi.org/10.3390/cells12192408

It is widely recognised that cells respond to their microenvironment, which has implications for cell culture practices. Growth cues provided by 2D cell culture substrates are far removed from native 3D tissue structure in vivo. Geometry is one of ma... Read More about Impact of the Physical Cellular Microenvironment on the Structure and Function of a Model Hepatocyte Cell Line for Drug Toxicity Applications.

Generation of a novel three-dimensional scaffold-based model of the bovine endometrium (2023)
Journal Article
Díez, M., Przyborski, S., del Cerro, A., Alonso-Guervós, M., Iglesias-Cabo, T., Carrocera, S., …Muñoz, M. (2023). Generation of a novel three-dimensional scaffold-based model of the bovine endometrium. Veterinary Research Communications, 47(3), 1721-1733. https://doi.org/10.1007/s11259-023-10130-0

Bovine in vitro endometrial models that resemble tissue function in vivo are needed to study infertility, long-term uterine alterations induced by pathogens and impact of endocrine disruptor chemicals on reproductive function and other reproductive s... Read More about Generation of a novel three-dimensional scaffold-based model of the bovine endometrium.

Quantitative morphometric analysis of intrinsic and extrinsic skin ageing in individuals with Fitzpatrick skin types II–III (2023)
Journal Article
Costello, L., Goncalves, K., De Los Santos Gomez, P., Simpson, A., Maltman, V., Ritchie, P., Tasseff, R., Isfort, R., Dicolandrea, T., Wei, X., Määttä, A., Karakesisoglou, I., Markiewicz, E., Bascom, C. C., & Przyborski, S. (2023). Quantitative morphometric analysis of intrinsic and extrinsic skin ageing in individuals with Fitzpatrick skin types II–III. Experimental Dermatology, 32(5), 620-631. https://doi.org/10.1111/exd.14754

Skin ageing is an intricate physiological process affected by intrinsic and extrinsic factors. There is a demand to understand how the skin changes with age and photoexposure in individuals with Fitzpatrick skin types I-III due to accelerated photoag... Read More about Quantitative morphometric analysis of intrinsic and extrinsic skin ageing in individuals with Fitzpatrick skin types II–III.

Regulation of epidermal proliferation and hair follicle cycling by synthetic photostable retinoid EC23 (2023)
Journal Article
Määttä, A., Nixon, R., Robinson, N., Ambler, C. A., Goncalves, K., Maltman, V., & Przyborski, S. (2023). Regulation of epidermal proliferation and hair follicle cycling by synthetic photostable retinoid EC23. Journal of Cosmetic Dermatology, 22(5), https://doi.org/10.1111/jocd.15629

Background Retinoid signaling is an important regulator of the epidermis and skin appendages. Therefore, synthetic retinoids have been developed for therapeutic use for skin disorders such as psoriasis and acne. Aims In previous studies, we showed ho... Read More about Regulation of epidermal proliferation and hair follicle cycling by synthetic photostable retinoid EC23.

Application of biomimetic surfaces and 3D culture technology to study the role of extracellular matrix interactions in neurite outgrowth and inhibition (2022)
Journal Article
Goncalves, K., Phillips, S., Shah, D., Athey, D., & Przyborski, S. (2023). Application of biomimetic surfaces and 3D culture technology to study the role of extracellular matrix interactions in neurite outgrowth and inhibition. Biomaterials Advances, 144, Article 213204. https://doi.org/10.1016/j.bioadv.2022.213204

The microenvironment that cells experience during in vitro culture can often be far removed from the native environment they are exposed to in vivo. To recreate the physiological environment that developing neurites experience in vivo, we combine a w... Read More about Application of biomimetic surfaces and 3D culture technology to study the role of extracellular matrix interactions in neurite outgrowth and inhibition.

Investigation into the effect of skin tone modulators and exogenous stress on skin pigmentation utilizing a novel bioengineered skin equivalent (2022)
Journal Article
Goncalves, K., De Los Santos Gomez, P., Costello, L., Smith, L., Mead, H., Simpson, A., & Przyborski, S. (2023). Investigation into the effect of skin tone modulators and exogenous stress on skin pigmentation utilizing a novel bioengineered skin equivalent. Bioengineering and Translational Medicine, 8(2), Article e10415. https://doi.org/10.1002/btm2.10415

Human skin equivalents (HSEs) are a popular technology due to limitations in animal testing, particularly as they recapitulate aspects of structure and function of human skin. Many HSEs contain two basic cell types to model dermal and epidermal compa... Read More about Investigation into the effect of skin tone modulators and exogenous stress on skin pigmentation utilizing a novel bioengineered skin equivalent.

Design considerations of benchtop fluid flow bioreactors for bio-engineered tissue equivalents in vitro (2022)
Journal Article
Hoyle, H., Stenger, C., & Przyborski, S. (2022). Design considerations of benchtop fluid flow bioreactors for bio-engineered tissue equivalents in vitro. Biomaterials and biosystems, 8, Article 100063. https://doi.org/10.1016/j.bbiosy.2022.100063

One of the major aims of bio-engineering tissue equivalents in vitro is to create physiologically relevant culture conditions to accurately recreate the cellular microenvironment. This often includes incorporation of factors such as the extracellular... Read More about Design considerations of benchtop fluid flow bioreactors for bio-engineered tissue equivalents in vitro.

Early onset of senescence and imbalanced epidermal homeostasis across the decades in photoexposed human skin: Fingerprints of inflammaging (2022)
Journal Article
Jarrold, B. B., Tan, C. Y. R., Ho, C. Y., Soon, A. L., Lam, T. T., Yang, X., Nguyen, C., Guo, W., Chew, Y. C., DeAngelis, Y. M., Costello, L., De Los Santos Gomez, P., Przyborski, S., Bellanger, S., Dreesen, O., Kimball, A. B., & Oblong, J. E. (2022). Early onset of senescence and imbalanced epidermal homeostasis across the decades in photoexposed human skin: Fingerprints of inflammaging. Experimental Dermatology, 31(11), 1748-1760. https://doi.org/10.1111/exd.14654

Inflammaging is a theory of ageing which purports that low-level chronic inflammation leads to cellular dysfunction and premature ageing of surrounding tissue. Skin is susceptible to inflammaging because it is the first line of defence from the envir... Read More about Early onset of senescence and imbalanced epidermal homeostasis across the decades in photoexposed human skin: Fingerprints of inflammaging.