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Evaluating Strategies to Assess the Differentiation Potential of Human Pluripotent Stem Cells: A Review, Analysis and Call for Innovation

Smith, Lucy; Quelch-Cliffe, Rebecca; Liu, Felicity; Aguilar, Alejandro Hidalgo; Przyborski, Stefan

Evaluating Strategies to Assess the Differentiation Potential of Human Pluripotent Stem Cells: A Review, Analysis and Call for Innovation Thumbnail


Authors

Rebecca Quelch-Cliffe

Felicity Liu



Abstract

Pluripotent stem cells have the ability to differentiate into all cells and tissues within the human body, and as a result they are attractive resources for use in basic research, drug discovery and regenerative medicine. In order to successfully achieve this application, starting cell sources ideally require in-depth characterisation to confirm their pluripotent status and their ability to differentiate into tissues representative of the three developmental germ layers. Many different methods to assess potency are employed, each having its own distinct advantages and limitations. Some aspects of this characterisation process are not always well standardised, particularly techniques used to assess pluripotency as a function. In this article, we consider the methods used to establish cellular pluripotency and subsequently analyse characterisation data for over 1590 human pluripotent cell lines from publicly available repositories in the UK and USA. In particular, we focus on the teratoma xenograft assay, its use and protocols, demonstrating the level of variation and the frequency with which it is used. Finally, we reflect on the implications of the findings, and suggest in vitro alternatives using modern innovative technology as a way forward.

Citation

Smith, L., Quelch-Cliffe, R., Liu, F., Aguilar, A. H., & Przyborski, S. (online). Evaluating Strategies to Assess the Differentiation Potential of Human Pluripotent Stem Cells: A Review, Analysis and Call for Innovation. Stem Cell Reviews and Reports, https://doi.org/10.1007/s12015-024-10793-5

Journal Article Type Article
Acceptance Date Sep 23, 2024
Online Publication Date Sep 28, 2024
Deposit Date Oct 9, 2024
Publicly Available Date Oct 9, 2024
Journal Stem Cell Reviews and Reports
Print ISSN 2629-3269
Electronic ISSN 2629-3277
Publisher Springer
Peer Reviewed Peer Reviewed
DOI https://doi.org/10.1007/s12015-024-10793-5
Public URL https://durham-repository.worktribe.com/output/2952308

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