Dr Lucy Smith l.a.smith2@durham.ac.uk
Academic Visitor
Evaluating Strategies to Assess the Differentiation Potential of Human Pluripotent Stem Cells: A Review, Analysis and Call for Innovation
Smith, Lucy; Quelch-Cliffe, Rebecca; Liu, Felicity; Aguilar, Alejandro Hidalgo; Przyborski, Stefan
Authors
Rebecca Quelch-Cliffe
Felicity Liu
Alejandro Hidalgo Aguilar alejandro.hidalgo-aguilar@durham.ac.uk
PGR Student Doctor of Philosophy
Professor Stefan Przyborski stefan.przyborski@durham.ac.uk
Deputy Provost
Abstract
Pluripotent stem cells have the ability to differentiate into all cells and tissues within the human body, and as a result they are attractive resources for use in basic research, drug discovery and regenerative medicine. In order to successfully achieve this application, starting cell sources ideally require in-depth characterisation to confirm their pluripotent status and their ability to differentiate into tissues representative of the three developmental germ layers. Many different methods to assess potency are employed, each having its own distinct advantages and limitations. Some aspects of this characterisation process are not always well standardised, particularly techniques used to assess pluripotency as a function. In this article, we consider the methods used to establish cellular pluripotency and subsequently analyse characterisation data for over 1590 human pluripotent cell lines from publicly available repositories in the UK and USA. In particular, we focus on the teratoma xenograft assay, its use and protocols, demonstrating the level of variation and the frequency with which it is used. Finally, we reflect on the implications of the findings, and suggest in vitro alternatives using modern innovative technology as a way forward.
Citation
Smith, L., Quelch-Cliffe, R., Liu, F., Aguilar, A. H., & Przyborski, S. (online). Evaluating Strategies to Assess the Differentiation Potential of Human Pluripotent Stem Cells: A Review, Analysis and Call for Innovation. Stem Cell Reviews and Reports, https://doi.org/10.1007/s12015-024-10793-5
Journal Article Type | Article |
---|---|
Acceptance Date | Sep 23, 2024 |
Online Publication Date | Sep 28, 2024 |
Deposit Date | Oct 9, 2024 |
Publicly Available Date | Oct 9, 2024 |
Journal | Stem Cell Reviews and Reports |
Print ISSN | 2629-3269 |
Electronic ISSN | 2629-3277 |
Publisher | Springer |
Peer Reviewed | Peer Reviewed |
DOI | https://doi.org/10.1007/s12015-024-10793-5 |
Public URL | https://durham-repository.worktribe.com/output/2952308 |
Files
Published Journal Article (Advance Online Version)
(3.4 Mb)
PDF
Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/
You might also like
How good is the evidence that cellular senescence causes skin ageing?
(2021)
Journal Article
Downloadable Citations
About Durham Research Online (DRO)
Administrator e-mail: dro.admin@durham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2025
Advanced Search