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Identification of cambium stem cell factors and their positioning mechanism

Eswaran, Gugan; Zhang, Xixi; Rutten, Jacob Pieter; Han, Jingyi; Iida, Hiroyuki; Lopez Ortiz, Jennifer; Mäkilä, Riikka; Wybouw, Brecht; Planterose Jiménez, Benjamin; Vainio, Leo; Porcher, Alexis; Gavarron, Marina Leal; Zhang, Jing; Blomster, Tiina; Dolan, David; Smetana, Ondřej; Brady, Siobhán M; Topcu, Melis Kucukoglu; Ten Tusscher, Kirsten; Etchells, J Peter; Mähönen, Ari Pekka

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Authors

Gugan Eswaran

Xixi Zhang

Jacob Pieter Rutten

Hiroyuki Iida

Jennifer Lopez Ortiz

Riikka Mäkilä

Brecht Wybouw

Benjamin Planterose Jiménez

Leo Vainio

Alexis Porcher

Marina Leal Gavarron

Jing Zhang

Tiina Blomster

Ondřej Smetana

Siobhán M Brady

Melis Kucukoglu Topcu

Kirsten Ten Tusscher

Ari Pekka Mähönen



Abstract

Wood constitutes the largest reservoir of terrestrial biomass. Composed of xylem, it arises from one side of the vascular cambium, a bifacial stem cell niche that also produces phloem on the opposing side. It is currently unknown which molecular factors endow cambium stem cell identity. Here we show that TDIF ligand-activated PXY receptors promote the expression of CAMBIUM-EXPRESSED AINTEGUMENTA-LIKE (CAIL) transcription factors to define cambium stem cell identity in the Arabidopsis root. By sequestrating the phloem-originated TDIF, xylem-expressed PXY confines the TDIF signaling front, resulting in the activation of CAIL expression and stem cell identity in only a narrow domain. Our findings show how signals emanating from cells on opposing sides ensure robust yet dynamically adjustable positioning of a bifacial stem cell layer.

Citation

Eswaran, G., Zhang, X., Rutten, J. P., Han, J., Iida, H., Lopez Ortiz, J., Mäkilä, R., Wybouw, B., Planterose Jiménez, B., Vainio, L., Porcher, A., Gavarron, M. L., Zhang, J., Blomster, T., Dolan, D., Smetana, O., Brady, S. M., Topcu, M. K., Ten Tusscher, K., Etchells, J. P., & Mähönen, A. P. (2024). Identification of cambium stem cell factors and their positioning mechanism. Science, 386(6722), 646-653. https://doi.org/10.1126/science.adj8752

Journal Article Type Article
Acceptance Date Sep 23, 2024
Online Publication Date Nov 7, 2024
Publication Date Nov 8, 2024
Deposit Date Nov 15, 2024
Publicly Available Date Nov 15, 2024
Journal Science
Print ISSN 0036-8075
Electronic ISSN 1095-9203
Publisher American Association for the Advancement of Science
Peer Reviewed Peer Reviewed
Volume 386
Issue 6722
Pages 646-653
DOI https://doi.org/10.1126/science.adj8752
Public URL https://durham-repository.worktribe.com/output/3095452

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