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The first intron of ARF7 is required for expression in root tips.

Han, Jingyi; Welch, Thomas; Voß, Ute; Vernoux, Teva; Bhosale, Rahul; Bishopp, Anthony

The first intron of ARF7 is required for expression in root tips. Thumbnail


Authors

Jingyi Han jingyi.han@durham.ac.uk
Post Doctoral Research Associate

Thomas Welch

Ute Voß

Teva Vernoux

Rahul Bhosale

Anthony Bishopp



Abstract

Auxin regulates plant growth and development through the transcription factors of the AUXIN RESPONSE FACTOR (ARF) gene family. ARF7 is one of five activators that bind DNA and elicit downstream transcriptional responses. In roots, ARF7 regulates growth, gravitropism and redundantly with ARF19, lateral root organogenesis. In this study we analyzed -regulation, using different non-coding sequences of the locus to drive GFP. We show that constructs containing the first intron led to increased signal in the root tip. Although bioinformatics analyses predicted several transcription factor binding sites in the first intron, we were unable to significantly alter expression of GFP in the root by mutating these. We instead observed the intronic sequences needed to be present within the transcribed sequences to drive expression in the root meristem. These data support a mechanism by which intron-mediated enhancement regulates the tissue specific expression of in the root meristem.

Journal Article Type Article
Acceptance Date May 6, 2024
Online Publication Date May 8, 2024
Publication Date Jun 21, 2024
Deposit Date Jun 25, 2024
Publicly Available Date Jun 25, 2024
Journal iScience
Publisher Cell Press
Peer Reviewed Peer Reviewed
Volume 27
Issue 6
Article Number 109936
DOI https://doi.org/10.1016/j.isci.2024.109936
Public URL https://durham-repository.worktribe.com/output/2493133

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