Germ fate determinants protect germ precursor cell division by reducing septin and anillin levels at the cell division plane.

Connors, Caroline Q; Mauro, Michael S; Wiles, J Tristian; Countryman, Andrew D; Martin, Sophia L; Lacroix, Benjamin; Shirasu-Hiza, Mimi; Dumont, Julien; Kasza, Karen E; Davies, Timothy R; Canman, Julie C

Abstract

Animal cell cytokinesis, or the physical division of one cell into two, is thought to be driven by constriction of an actomyosin contractile ring at the division plane. The mechanisms underlying cell type-specific differences in cytokinesis remain unknown. Germ cells are totipotent cells that pass genetic information to the next generation. Previously, using mutant embryos, we found that the P2 germ precursor cell is protected from cytokinesis failure and can divide with greatly reduced F-actin levels at the cell division plane. Here, we identified two canonical germ fate determinants required for P2-specific cytokinetic protection: PIE-1 and POS-1. Neither has been implicated previously in cytokinesis. These germ fate determinants protect P2 cytokinesis by reducing the accumulation of septin and anillin at the division plane, which here act as negative regulators of cytokinesis. These findings may provide insight into the regulation of cytokinesis in other cell types, especially in stem cells with high potency.

Citation

Connors, C. Q., Mauro, M. S., Wiles, J. T., Countryman, A. D., Martin, S. L., Lacroix, B., Shirasu-Hiza, M., Dumont, J., Kasza, K. E., Davies, T. R., & Canman, J. C. (2024). Germ fate determinants protect germ precursor cell division by reducing septin and anillin levels at the cell division plane. Molecular Biology of the Cell, 35(7), Article mbcE24020096T. https://doi.org/10.1091/mbc.E24-02-0096-T

• Journal Article Type: Article
• Acceptance Date: Apr 26, 2024
• Online Publication Date: Jun 6, 2024
• Publication Date: Jul 1, 2024
• Deposit Date: May 22, 2024
• Publicly Available Date: Aug 7, 2024
• Journal: Molecular Biology of the Cell
• Electronic ISSN: 1939-4586
• Publisher: American Society for Cell Biology
• Peer Reviewed: Peer Reviewed
• Volume: 35
• Issue: 7
• Article Number: mbcE24020096T
• DOI: https://doi.org/10.1091/mbc.E24-02-0096-T
• Public URL: https://durham-repository.worktribe.com/output/2450778