Cell-intrinsic and -extrinsic mechanisms promote cell-type-specific cytokinetic diversity

Davies, Tim; Kim, Han X; Romano Spica, Natalia; Lesea-Pringle, Benjamin J; Dumont, Julien; Shirasu-Hiza, Mimi; Canman, Julie C

Abstract

Cytokinesis, the physical division of one cell into two, is powered by constriction of an actomyosin contractile ring. It has long been assumed that all animal cells divide by a similar molecular mechanism, but growing evidence suggests that cytokinetic regulation in individual cell types has more variation than previously realized. In the four-cell Caenorhabditis elegans embryo, each blastomere has a distinct cell fate, specified by conserved pathways. Using fast-acting temperature-sensitive mutants and acute drug treatment, we identified cell-type-specific variation in the cytokinetic requirement for a robust forminCYK-1-dependent filamentous-actin (F-actin) cytoskeleton. In one cell (P2), this cytokinetic variation is cell-intrinsically regulated, whereas in another cell (EMS) this variation is cell-extrinsically regulated, dependent on both SrcSRC-1 signaling and direct contact with its neighbor cell, P2. Thus, both cell-intrinsic and -extrinsic mechanisms control cytokinetic variation in individual cell types and can protect against division failure when the contractile ring is weakened.

Citation

Davies, T., Kim, H. X., Romano Spica, N., Lesea-Pringle, B. J., Dumont, J., Shirasu-Hiza, M., & Canman, J. C. (2018). Cell-intrinsic and -extrinsic mechanisms promote cell-type-specific cytokinetic diversity. eLife, 7, Article e36204. https://doi.org/10.7554/elife.36204

• Journal Article Type: Article
• Acceptance Date: Jun 10, 2018
• Online Publication Date: Jul 20, 2018
• Publication Date: Jul 20, 2018
• Deposit Date: Jan 14, 2020
• Publicly Available Date: Feb 16, 2020
• Journal: eLife
• Electronic ISSN: 2050-084X
• Publisher: eLife Sciences Publications
• Peer Reviewed: Peer Reviewed
• Volume: 7
• Article Number: e36204
• DOI: https://doi.org/10.7554/elife.36204
• Public URL: https://durham-repository.worktribe.com/output/1273760