In Vitro Assessment of the Neuro-Compatibility of Fe-20Mn as a Potential Bioresorbable Material for Craniofacial Surgery

Ajami, Sara; Kraaneveld, Charlotte; Koudstaal, Maarten; Dunaway, David; Jeelani, Noor Ul Owase; Schievano, Silvia; Bregoli, Chiara; Fiocchi, Jacopo; Biffi, Carlo Alberto; Tuissi, Ausonio; Borghi, Alessandro; Schievano, Silvia; Kraaneveld, Charlotte; Koudstaal, Maarten; Tuissi, Ausonio

Abstract

Background and Objectives: Spring-assisted surgery is a popular option for the treatment of non-syndromic craniosynostosis. The main drawback of this procedure is the need for a second surgery for spring removal, which could be avoided if a distractor material could be metabolised over time. Iron–Manganese alloys (FeMn) have a good trade-off between degradation rate and strength; however, their biocompatibility is still debated. Materials and Methods: In this study, the neuro-compatibility of Fe-20Mn (wt.%) was assessed using standard assays. PC-12 cells were exposed to Fe-20Mn (wt.%) and stainless steel via indirect contact. To examine the cytotoxicity, a Cell Tox Green assay was carried out after 1, 2, and 3 days of incubation. Following differentiation, a neurite morphological examination after 1 and 7 days of incubation time was carried out. The degradation response in modified Hank’s solution at 1, 3, and 7 days was investigated, too. Results: The cytotoxicity assay showed a higher toxicity of Fe-20Mn than stainless steel at earlier time points; however, at the latest time point, no differences were found. Neurite morphology was similar for cells exposed to Fe-20Mn and stainless steel. Conclusions: In conclusion, the Fe-20Mn alloy shows promising neuro-compatibility. Future studies will focus on in vivo studies to confirm the cellular response to Fe-20Mn.

Citation

Ajami, S., Kraaneveld, C., Koudstaal, M., Dunaway, D., Jeelani, N. U. O., Schievano, S., Bregoli, C., Fiocchi, J., Biffi, C. A., Tuissi, A., Borghi, A., Schievano, S., Kraaneveld, C., Koudstaal, M., & Tuissi, A. (2024). In Vitro Assessment of the Neuro-Compatibility of Fe-20Mn as a Potential Bioresorbable Material for Craniofacial Surgery. Medicina, 60(3), 440. https://doi.org/10.3390/medicina60030440

• Journal Article Type: Article
• Acceptance Date: Mar 5, 2024
• Online Publication Date: Mar 7, 2024
• Publication Date: Mar 1, 2024
• Deposit Date: May 20, 2024
• Publicly Available Date: May 20, 2024
• Journal: Medicina
• Print ISSN: 1010-660X
• Electronic ISSN: 1648-9144
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 60
• Issue: 3
• Pages: 440
• DOI: https://doi.org/10.3390/medicina60030440
• Keywords: bioresorbable metals, Fe-20Mn alloys, cytotoxicity, biocompatibility, cell viability
• Public URL: https://durham-repository.worktribe.com/output/2378536