Yanlong Li
Single‐Cell Transcriptome Atlas and Regulatory Dynamics in Developing Cotton Anthers
Li, Yanlong; Ma, Huanhuan; Wu, Yuanlong; Ma, Yizan; Yang, Jing; Li, Yawei; Yue, Dandan; Zhang, Rui; Kong, Jie; Lindsey, Keith; Zhang, Xianlong; Min, Ling
Authors
Huanhuan Ma
Yuanlong Wu
Yizan Ma
Jing Yang
Yawei Li
Dandan Yue
Rui Zhang
Jie Kong
Professor Keith Lindsey keith.lindsey@durham.ac.uk
Professor
Xianlong Zhang
Ling Min
Abstract
Plant anthers are composed of different specialized cell types with distinct roles in plant reproduction. High temperature (HT) stress causes male sterility, resulting in crop yield reduction. However, the spatial expression atlas and regulatory dynamics during anther development and in response to HT remain largely unknown. Here, the first single‐cell transcriptome atlas and chromatin accessibility survey in cotton anther are established, depicting the specific expression and epigenetic landscape of each type of cell in anthers. The reconstruction of meiotic cells, tapetal cells, and middle layer cell developmental trajectories not only identifies novel expressed genes, but also elucidates the precise degradation period of middle layer and reveals a rapid function transition of tapetal cells during the tetrad stage. By applying HT, heterogeneity in HT response is shown among cells of anthers, with tapetal cells responsible for pollen wall synthesis are most sensitive to HT. Specifically, HT shuts down the chromatin accessibility of genes specifically expressed in the tapetal cells responsible for pollen wall synthesis, such as QUARTET 3 (QRT3) and CYTOCHROME P450 703A2 (CYP703A2), resulting in a silent expression of these genes, ultimately leading to abnormal pollen wall and male sterility. Collectively, this study provides substantial information on anthers and provides clues for heat‐tolerant crop creation.
Citation
Li, Y., Ma, H., Wu, Y., Ma, Y., Yang, J., Li, Y., …Min, L. (2023). Single‐Cell Transcriptome Atlas and Regulatory Dynamics in Developing Cotton Anthers. Advanced Science, https://doi.org/10.1002/advs.202304017
Journal Article Type | Article |
---|---|
Acceptance Date | Oct 9, 2023 |
Online Publication Date | Nov 17, 2023 |
Publication Date | 2023 |
Deposit Date | Nov 20, 2023 |
Publicly Available Date | Nov 20, 2023 |
Journal | Advanced Science |
Publisher | Wiley Open Access |
Peer Reviewed | Peer Reviewed |
DOI | https://doi.org/10.1002/advs.202304017 |
Keywords | high temperature, single‐cell multi‐omics, single‐cell RNA sequencing (scRNA‐seq), anthers, tapetum |
Public URL | https://durham-repository.worktribe.com/output/1932326 |
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http://creativecommons.org/licenses/by/4.0/
Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/
Copyright Statement
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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