Biophysical insights from supported lipid patches

Miller, Ethan; Stubbington, Liam; Dinet, Celine; Staykova, Margarita

Abstract

Supported lipid bilayer (SLB) patches have emerged in recent years as a convenient system to study membrane-substrate interactions, membrane mechanics, and interaction of membranes with other molecules, due to the possibility of tracking their area changes in response to various stimuli. In this paper, we present protocols for the formation of SLB patches on glass and polydimethylsiloxane substrates via vesicle fusion, describe the interaction forces between the lipid membrane and the substrate, and discuss the force dependence on the buffer composition, membrane lipid species, the surface properties of the substrate. Following that, we present own and others results that utilize SLB patches to study 1) membrane sorption of cholesterol, peptides and other surface active molecules, 2) the effects of the substrate on lipid phase separation, and 3) the mechanics of substrate-adhered lipid membranes and their biophysical implications. Finally we discuss methods to obtain SLB patches of more complex composition and geometry.

Citation

Miller, E., Stubbington, L., Dinet, C., & Staykova, M. (2019). Biophysical insights from supported lipid patches. In A. Iglič, M. Rappolt, & A. J. García-Sáez (Eds.), Advances in Biomembranes and Lipid Self-Assembly (23-48). Elsevier. https://doi.org/10.1016/bs.abl.2019.01.004

• Online Publication Date: Feb 20, 2019
• Publication Date: 2019
• Deposit Date: Mar 27, 2019
• Publisher: Elsevier
• Pages: 23-48
• Series Number: 29
• Book Title: Advances in Biomembranes and Lipid Self-Assembly.
• DOI: https://doi.org/10.1016/bs.abl.2019.01.004
• Public URL: https://durham-repository.worktribe.com/output/1632288
• Contract Date: Jan 16, 2019