M.V. Monsalve
Brief communication: Molecular analysis of the Kwäday Dän Ts'finchi ancient remains found in a glacier in Canada
Monsalve, M.V.; Stone, A.C.; Lewis, C.M.; Rempel, A.; Richards, M.P.; Straathof, S.; Devine, D.V.
Authors
A.C. Stone
C.M. Lewis
A. Rempel
M.P. Richards
S. Straathof
D.V. Devine
Abstract
DNA was extracted from the frozen remains of a man found in the northwest corner of British Columbia, Canada, in 1999. His clothing was radiocarbon-dated at ca. 550 years old. Nitrogen and carbon content in whole bone and collagen-type residue extracted from both bone and muscle indicated good preservation of proteinaceous macromolecules. Restriction enzyme analysis of mitochondrial DNA (mtDNA) determined that the remains belong to haplogroup A, one of the four major Native American mtDNA haplogroups. Data obtained by PCR direct sequencing of the mtDNA control region, and by sequencing the clones from overlapping PCR products, were duplicated by an independent laboratory. Comparison of these mtDNA sequences with those of North American, Central American, South American, East Siberian, Greenlandic, and Northeast Asian populations indicates that the remains share an mtDNA type with North American, Central American, and South American populations.
Citation
Monsalve, M., Stone, A., Lewis, C., Rempel, A., Richards, M., Straathof, S., & Devine, D. (2002). Brief communication: Molecular analysis of the Kwäday Dän Ts'finchi ancient remains found in a glacier in Canada. American journal of physical anthropology, 119(3), 288-291. https://doi.org/10.1002/ajpa.10116
Journal Article Type | Article |
---|---|
Publication Date | Nov 1, 2002 |
Deposit Date | Jul 20, 2009 |
Journal | American Journal of Physical Anthropology |
Print ISSN | 0002-9483 |
Electronic ISSN | 1096-8644 |
Publisher | Wiley |
Peer Reviewed | Not Peer Reviewed |
Volume | 119 |
Issue | 3 |
Pages | 288-291 |
DOI | https://doi.org/10.1002/ajpa.10116 |
Keywords | Ancient DNA, Native American, mtDNA haplogroups, Control region, Biochemical analysis. |
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