M. van Lith
A developmentally regulated chaperone complex for the endoplasmic reticulum of male haploid germ cells
van Lith, M.; Karala, A.; Bown, D.; Gatehouse, J.; Ruddock, L.; Saunders, P; Benham, AM.
Authors
A. Karala
D. Bown
J. Gatehouse
L. Ruddock
P Saunders
Professor Adam Benham adam.benham@durham.ac.uk
Professor
Abstract
Glycoprotein folding is mediated by lectin-like chaperones and protein disulfide isomerases (PDIs) in the endoplasmic reticulum (ER). Calnexin and the PDI homologue ERp57 work together to help fold nascent polypeptides with glycans located toward the N-terminus of a protein, whereas PDI and BiP may engage proteins that lack glycans or have sugars toward the C-terminus. In this study, we show that the PDI homologue PDILT is expressed exclusively in post-meiotic male germ cells, in contrast to the ubiquitous expression of many other PDI family members in the testis. PDILT is induced during puberty and represents the first example of a PDI family member under developmental control. We find that PDILT is not active as an oxido-reductase, but interacts with the model peptide -somatostatin and nonnative BPTI in vitro, indicative of chaperone activity. In vivo, PDILT forms a tissue-specific chaperone complex with the calnexin homologue calmegin. The identification of a redox-inactive chaperone partnership defines a new system of testis-specific protein folding with implications for male fertility.
Citation
van Lith, M., Karala, A., Bown, D., Gatehouse, J., Ruddock, L., Saunders, P., & Benham, A. (2007). A developmentally regulated chaperone complex for the endoplasmic reticulum of male haploid germ cells. Molecular Biology of the Cell, 18(8), 2795-2804. https://doi.org/10.1091/mbc.e07-02-0147
Journal Article Type | Article |
---|---|
Publication Date | Aug 1, 2007 |
Deposit Date | Oct 1, 2008 |
Publicly Available Date | Oct 1, 2008 |
Journal | Molecular biology of the cell |
Publisher | American Society for Cell Biology |
Peer Reviewed | Peer Reviewed |
Volume | 18 |
Issue | 8 |
Pages | 2795-2804 |
DOI | https://doi.org/10.1091/mbc.e07-02-0147 |
Files
Published Journal Article
(398 Kb)
PDF
Copyright Statement
© 2007 by The American Society for Cell Biology
You might also like
Insecticidal effects of dsRNA targeting the Diap1 gene in dipteran pests
(2017)
Journal Article
Downloadable Citations
About Durham Research Online (DRO)
Administrator e-mail: dro.admin@durham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search