A.A. Poll
Septoria Leaf Blotch and Reduced Nitrogen Availability Alter WRKY Transcription Factor Expression in a Codependent Manner
Poll, A.A.; Lee, J.; Sanderson, R.A.; Byrne, E.; Gatehouse, J.A.; Sadanandom, A.; Gatehouse, A.M.R.; Edwards, M.G.
Authors
J. Lee
R.A. Sanderson
E. Byrne
J.A. Gatehouse
A. Sadanandom
A.M.R. Gatehouse
M.G. Edwards
Abstract
A major cause of yield loss in wheat worldwide is the fungal pathogen Zymoseptoria tritici, a hemibiotrophic fungus which causes Septoria leaf blotch, the most destructive wheat disease in Europe. Resistance in commercial wheat varieties is poor, however, a link between reduced nitrogen availability and increased Septoria tolerance has been observed. We have shown that Septoria load is not affected by nitrogen, whilst the fungus is in its first, symptomless stage of growth. This suggests that a link between nitrogen and Septoria is only present during the necrotrophic phase of Septoria infection. Quantitative real-time PCR data demonstrated that WRKYs, a superfamily of plant-specific transcription factors, are differentially expressed in response to both reduced nitrogen and Septoria. WRKY39 was downregulated over 30-fold in response to necrotrophic stage Septoria, whilst changes in the expression of WRKY68a during the late biotrophic phase were dependent on the concentration of nitrogen under which wheat is grown. WRKY68a may therefore mediate a link between nitrogen and Septoria. The potential remains to identify key regulators in the link between nitrogen and Septoria, and as such, elucidate molecular markers for wheat breeding, or targets for molecular-based breeding approaches.
Citation
Poll, A., Lee, J., Sanderson, R., Byrne, E., Gatehouse, J., Sadanandom, A., Gatehouse, A., & Edwards, M. (2020). Septoria Leaf Blotch and Reduced Nitrogen Availability Alter WRKY Transcription Factor Expression in a Codependent Manner. International Journal of Molecular Sciences, 21(11), Article 4165. https://doi.org/10.3390/ijms21114165
Journal Article Type | Article |
---|---|
Acceptance Date | Jun 8, 2020 |
Online Publication Date | Jun 11, 2020 |
Publication Date | 2020-06 |
Deposit Date | Jun 12, 2020 |
Publicly Available Date | Jun 12, 2020 |
Journal | International Journal of Molecular Sciences |
Print ISSN | 1661-6596 |
Publisher | MDPI |
Peer Reviewed | Peer Reviewed |
Volume | 21 |
Issue | 11 |
Article Number | 4165 |
DOI | https://doi.org/10.3390/ijms21114165 |
Public URL | https://durham-repository.worktribe.com/output/1300113 |
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Copyright Statement
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution
(CC BY) license http://creativecommons.org/licenses/by/4.0/).
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