A cationic lanthanide complex binds selectively to phosphorylated tyrosine sites, aiding NMR analysis of the phosphorylated insulin receptor peptide fragment

Atkinson, P.; Murray, B.S.; Parker, D.

Abstract

The binding of two cationic europium complexes to a differentially phosphorylated insulin receptor peptide has been studied by emission spectroscopy and 31P NMR and 1H NMR TOCSY methods. Analysis of the europium emission and NMR spectral data was consistent with the presence of species in slow exchange on the NMR and emission timescales, in agreement with selective binding of the lanthanide ion to the phospho-tyrosine site, allowing such complexes to be considered as prototypical chemoselective paramagnetic derivatising agents.

Citation

Atkinson, P., Murray, B., & Parker, D. (2006). A cationic lanthanide complex binds selectively to phosphorylated tyrosine sites, aiding NMR analysis of the phosphorylated insulin receptor peptide fragment. Organic and Biomolecular Chemistry, 4(16), 3166-3171. https://doi.org/10.1039/b608528h

• Journal Article Type: Article
• Acceptance Date: Jun 30, 2006
• Online Publication Date: Jul 19, 2006
• Publication Date: 2006
• Journal: Organic and Biomolecular Chemistry
• Print ISSN: 1477-0520
• Electronic ISSN: 1477-0539
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 4
• Issue: 16
• Pages: 3166-3171
• DOI: https://doi.org/10.1039/b608528h
• Public URL: https://durham-repository.worktribe.com/output/1542189