Engineering eukaryotic protein factories

Schröder, M.

Abstract

The biopharmaceuticals market is currently outperforming the pharmaceuticals market and is now valued at US$ 48 billion with an average annual growth of 19%. Behind this success is a 100-fold increase in productivities of eukaryotic expression systems. However, the productivity per cell has remained unchanged for more than 10 years. The engineering of the ER-resident protein folding machinery is discussed together with an overview of signal transduction pathways activated by heterologous protein overexpression to increase cell specific productivities.

Citation

Schröder, M. (2008). Engineering eukaryotic protein factories. Biotechnology Letters, 30(2), 187-196. https://doi.org/10.1007/s10529-007-9524-1

• Journal Article Type: Article
• Publication Date: Feb 1, 2008
• Deposit Date: May 31, 2012
• Publicly Available Date: Jun 12, 2012
• Journal: Biotechnology Letters
• Print ISSN: 0141-5492
• Electronic ISSN: 1573-6776
• Publisher: Springer
• Peer Reviewed: Peer Reviewed
• Volume: 30
• Issue: 2
• Pages: 187-196
• DOI: https://doi.org/10.1007/s10529-007-9524-1
• Keywords: Unfolded protein response, Endoplasmic reticulum, Molecular chaperone, Recombinant protein production, Heterologous protein production, Protein folding.
• Public URL: https://durham-repository.worktribe.com/output/1528253