Momoko Ikeuchi
PRC2 represses dedifferentiation of mature somatic cells in Arabidopsis
Ikeuchi, Momoko; Iwase, Akira; Rymen, Bart; Harashima, Hirofumi; Shibata, Michitaro; Ohnuma, Mariko; Breuer, Christian; Morao, Ana Karina; de Lucas, Miguel; De Veylder, Lieven; Goodrich, Justin; Brady, Siobhan M.; Roudier, François; Sugimoto, Keiko
Authors
Akira Iwase
Bart Rymen
Hirofumi Harashima
Michitaro Shibata
Mariko Ohnuma
Christian Breuer
Ana Karina Morao
Dr Miguel De Lucas Torres miguel.de-lucas@durham.ac.uk
Associate Professor
Lieven De Veylder
Justin Goodrich
Siobhan M. Brady
François Roudier
Keiko Sugimoto
Abstract
Plant somatic cells are generally acknowledged to retain totipotency, the potential to develop into any cell type within an organism. This astonishing plasticity may contribute to a high regenerative capacity on severe damage, but how plants control this potential during normal post-embryonic development remains largely unknown1,2. Here we show that POLYCOMB REPRESSIVE COMPLEX 2 (PRC2), a chromatin regulator that maintains gene repression through histone modification, prevents dedifferentiation of mature somatic cells in Arabidopsis thaliana roots. Loss-of-function mutants in PRC2 subunits initially develop unicellular root hairs indistinguishable from those in wild type but fail to retain the differentiated state, ultimately resulting in the generation of an unorganized cell mass and somatic embryos from a single root hair. Strikingly, mutant root hairs complete the normal endoreduplication programme, increasing their nuclear ploidy, but subsequently reinitiate mitotic division coupled with successive DNA replication. Our data show that the WOUND INDUCED DEDIFFERENTIATION3 (WIND3) and LEAFY COTYLEDON2 (LEC2) genes are among the PRC2 targets involved in this reprogramming, as their ectopic overexpression partly phenocopies the dedifferentiation phenotype of PRC2 mutants. These findings unveil the pivotal role of PRC2-mediated gene repression in preventing unscheduled reprogramming of fully differentiated plant cells.
Citation
Ikeuchi, M., Iwase, A., Rymen, B., Harashima, H., Shibata, M., Ohnuma, M., …Sugimoto, K. (2015). PRC2 represses dedifferentiation of mature somatic cells in Arabidopsis. Nature Plants, 1(7), Article 15089. https://doi.org/10.1038/nplants.2015.89
Journal Article Type | Article |
---|---|
Acceptance Date | May 29, 2015 |
Publication Date | Jul 1, 2015 |
Deposit Date | Nov 11, 2015 |
Publicly Available Date | Mar 18, 2016 |
Journal | Nature Plants |
Print ISSN | 2055-026X |
Electronic ISSN | 2055-0278 |
Publisher | Nature Research |
Peer Reviewed | Peer Reviewed |
Volume | 1 |
Issue | 7 |
Article Number | 15089 |
DOI | https://doi.org/10.1038/nplants.2015.89 |
Public URL | https://durham-repository.worktribe.com/output/1426990 |
Files
Accepted Journal Article
(7 Mb)
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