Skip to main content

Research Repository

Advanced Search

Combining modelling and experimental approaches to explain how calcium signatures are decoded by calmodulin-binding transcription activators (CAMTAs) to produce specific gene expression responses

Liu, J.; Whalley, H.J.; Knight, M.R.

Combining modelling and experimental approaches to explain how calcium signatures are decoded by calmodulin-binding transcription activators (CAMTAs) to produce specific gene expression responses Thumbnail


Authors

H.J. Whalley



Abstract

Experimental data show that Arabidopsis thaliana is able to decode different calcium signatures to produce specific gene expression responses. It is also known that calmodulin-binding transcription activators (CAMTAs) have calmodulin (CaM)-binding domains. Therefore, the gene expression responses regulated by CAMTAs respond to calcium signals. However, little is known about how different calcium signatures are decoded by CAMTAs to produce specific gene expression responses. A dynamic model of Ca2+–CaM–CAMTA binding and gene expression responses is developed following thermodynamic and kinetic principles. The model is parameterized using experimental data. Then it is used to analyse how different calcium signatures are decoded by CAMTAs to produce specific gene expression responses. Modelling analysis reveals that: calcium signals in the form of cytosolic calcium concentration elevations are nonlinearly amplified by binding of Ca2+, CaM and CAMTAs; amplification of Ca2+ signals enables calcium signatures to be decoded to give specific CAMTA-regulated gene expression responses; gene expression responses to a calcium signature depend upon its history and accumulate all the information during the lifetime of the calcium signature. Information flow from calcium signatures to CAMTA-regulated gene expression responses has been established by combining experimental data with mathematical modelling.

Journal Article Type Article
Acceptance Date Mar 26, 2015
Online Publication Date Apr 27, 2015
Publication Date Oct 1, 2015
Deposit Date May 8, 2015
Publicly Available Date May 27, 2015
Journal New Phytologist
Print ISSN 0028-646X
Electronic ISSN 1469-8137
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 208
Issue 1
Pages 174-187
DOI https://doi.org/10.1111/nph.13428
Keywords Arabidopsis, Ccalcium signatures, Calmodulin (CaM), Calmodulin-binding transcription activators (CAMTAs), Gene expression, Mathematical modelling.
Public URL https://durham-repository.worktribe.com/output/1405970

Files



Accepted Journal Article (1.1 Mb)
PDF

Copyright Statement
© 2015 The Authors. New Phytologist © 2015 New Phytologist Trust
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.






You might also like



Downloadable Citations